FastQCFastQC Report
Tue 10 May 2022
EGAF00003659022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15368
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA1430.9305049453409683No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT830.5400832899531494No Hit
GTAGTAGACAGCCTTGCTCGTGGTTGTTGCGGACGTTGCGCTTCCGGCCG410.2667881311816762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.2407600208224883No Hit
GTACATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGG290.18870380010411245No Hit
GTCTACTACAACGCCCTGTTCGACGTGCCCAACGACCACGGAATCCTGCG290.18870380010411245No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA270.17568974492451847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTGGAAGTGGGGGCTAGTGGGAGGTGTTTGGGTCATGGGGACAGATC240.15616866215512754No Hit
GTCTTGTTCTGTCCCCCAGGCTGGAGTGCAATGGCGCGATCTCAGCTCAA230.14966163456533055No Hit
GTATGAGATTGGTCATTTTCCTTCTGCCTTTTGAAGACCGTGCTCCAATG210.1366475793857366No Hit
TGTGATGACCGTGCGCGCCGAGATCTCCGAGGCCGACGTGAACCTCGTGG210.1366475793857366No Hit
GTACATGGGGTGTTTGAGGCCCTCATGGTTTGGATCAAGCATGACCTCCA210.1366475793857366No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT200.1301405517959396Clontech Universal Primer Mix Long (96% over 26bp)
ATTATAAATAGTGCTGCTTTGCTTGGTGCGGTTGCTCACACCTGTAATCC200.1301405517959396No Hit
GTCTACATCTTCTCCCTGAAACTCAATCAGTGGAGGCTGGGGGAGCCCAT190.12363352420614263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.11712649661634565No Hit
CATGGGGACCGCGCAGGCCGACCTGGAGGCGGCCCAGGCGGCGGTGGATA170.11061946902654868No Hit
GTAACAAACTACCCCCAAAATCTAATGGCTTAAAGAGCAACAGTTATTTA170.11061946902654868No Hit
GTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCACAC170.11061946902654868No Hit
GCCTACCATAGCGCCGGACCCTGCGGCACGGCGGCATCGGCCCCGACCGG160.10411244143675169No Hit
ATGTATACATGTGCATGTGTGTGCAAGAGGGTGTGGGTGTGTCCATGCAC160.10411244143675169No Hit
TCTCAGTACAGTGCTCTTCCAAGATAAGGTTGGACTTTGCGCCGGTCATC160.10411244143675169No Hit
GTCCATAACTCTCTCTTAGAACCCTCAGTCTCCATAGAAGAAGCCCAGTT160.10411244143675169No Hit
GTACATGGGTGAGCCAAGACCCCACCTTTGCACTCCAGCCTGGGCAACAA160.10411244143675169No Hit
TGCAGGAACTGTTCAAGCAGGTCAGGCTGCAGTACATCCACCCAGCCTTC160.10411244143675169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)