FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52.262443438914027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52.262443438914027Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.809954751131222Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT41.809954751131222Clontech Universal Primer Mix Long (100% over 23bp)
CCACCGAGATCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCT41.809954751131222Illumina Single End PCR Primer 1 (97% over 38bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTT31.3574660633484164Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT31.3574660633484164Clontech SMART CDS Primer II A (100% over 26bp)
GACCCGCGTTGCCGTCCATTTCGCTTCACCGGTTTTGCGATCCCTCCTGT31.3574660633484164No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA31.3574660633484164Clontech Universal Primer Mix Long (95% over 21bp)
AGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGG20.904977375565611No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTTATACACA20.904977375565611Clontech SMART CDS Primer II A (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20.904977375565611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAGCTATAAGAAGTAAATATGTATATGGGTTAACAGCAACACCTACAA20.904977375565611No Hit
GTCGGACCCGACGCCGCGGTGCTGCCCGACCTCGTCAAGCCCAACGGCGA20.904977375565611No Hit
GGCTGCGCGACAGGATCGACGAGCGCTTCCGTCGCGGCACCAGAGGTCGC20.904977375565611No Hit
GTCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC20.904977375565611Illumina Single End PCR Primer 1 (97% over 37bp)
TGCAAATGGTGCTTGCTATGAGGCTTTAAAAAAGGCTTTAAGGATGAAAG20.904977375565611No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.904977375565611Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGTACACGACGTTTCCGATCTCTTGCCTCAGATTCTGTCGGTA20.904977375565611No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
ACACACGCTCTTCCGATCTATCCCTGTTGGTACCAAAAAAGGGATAGATC20.904977375565611No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGTTTCCCATGTACTCTGC20.904977375565611Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
CCGGCAACGCCGCTGGCGCAAAAGCCGGAGGCGTGAATGTCGGAACAGGC20.904977375565611No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAATACGGCGACACGAGATCTACACT20.904977375565611No Hit
ATCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.904977375565611Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCGGGCCGTTTCCAGCCGGCAACTCCGACGTTCTTGAACGCGGGTAA20.904977375565611No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACCTTGGGTGTTACCCCG10.4524886877828055No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCGCTTGCCTCAGATTCTGTCGCTGTCTCTTAT10.4524886877828055Illumina Single End PCR Primer 1 (95% over 23bp)
CTACACGACGCTCTTCCGACCTGTCCCAAGTTCTGCAGGTCGGAAGAGCG10.4524886877828055Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAGCGT10.4524886877828055No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGG10.4524886877828055No Hit
GGTATCAACGCAGAGTACATGGGTGTTGGGGGGGGGCGGGGTGTACACTA10.4524886877828055No Hit
TATCAACGCAGAGTACATGGATGATACGGCGACCCCGAGACTGTCTCTTA10.4524886877828055No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATAACGCAGAGTACATGG10.4524886877828055No Hit
ATCTGAATGATGATGTAAGAAGACCACTGTTCTAGATTTTAATAACAAAA10.4524886877828055No Hit
GTACATGGCAATGATACGGCGACCACCGAGATCTGTCTCTTATACACATC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 25bp)
CTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTGCT10.4524886877828055No Hit
TGCCAGCGCCTCGTCAAGCTGCGCCAGAGTACAAAGCCCGGTCAGTTTTT10.4524886877828055No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGTGCGTTTAAACCCAT10.4524886877828055No Hit
GTTGTTCTTGGAGGCTCTTTTTCATCTATTTCTTCAGTAATTCCTTGTAC10.4524886877828055No Hit
GAAAGTCAAAACTAGAAACAAGAGGTGTTTAAAGTTGCTGCAACTTTTTA10.4524886877828055No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCATGCTGTCTCTTATACA10.4524886877828055Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACAT10.4524886877828055Clontech Universal Primer Mix Long (96% over 28bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATCT10.4524886877828055Clontech Universal Primer Mix Long (96% over 25bp)
ATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.4524886877828055Illumina Single End PCR Primer 1 (100% over 37bp)
GTATCAACGCAGAGTACATGGGTGATACGCGACGCTCTTCCGATCTCATG10.4524886877828055Illumina Single End Sequencing Primer (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTCTCTTATACAC10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGATCTCGGTGGTCGCCGTACCATCCCATGTACTCTGCGTCTGTCT10.4524886877828055Illumina Single End PCR Primer 1 (96% over 28bp)
CTACACGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGGA10.4524886877828055Illumina Single End PCR Primer 1 (100% over 22bp)
TAAAAGCATATAACATATAAACAACTCCTATCAAACCAGACTACATTGCA10.4524886877828055No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATACGCGACT10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATAATCGTTTAT10.4524886877828055No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCCCGAGACTACACTCTTTCCCTACACGACGCTCTTCCGATC10.4524886877828055Illumina Single End PCR Primer 1 (100% over 34bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.4524886877828055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACAAAGGGTGCCAT10.4524886877828055No Hit
CATTTTTGCCCGGGAAACCCCATTTTTTGGCGACGAGACCCCTTCTCGTC10.4524886877828055No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGAGTACTT10.4524886877828055No Hit
CCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCATACCAGAACA10.4524886877828055Illumina Single End PCR Primer 1 (100% over 24bp)
AAAACAATATATCACTAAAATACACAAATCCGATCAAACCAAAATACATT10.4524886877828055No Hit
ACGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAACTGCGTTTTTA10.4524886877828055No Hit
GCAGTGGTATCAACGCAGAGTACATGGGACGCAGAGGAAACAACCCAGAG10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACTGC10.4524886877828055Clontech SMART CDS Primer II A (95% over 24bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTATGACGTTAT10.4524886877828055Clontech SMART CDS Primer II A (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACGCAGAGTCTGTCT10.4524886877828055No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAAAAAAAA10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAACATTAGTTGGAAAAGTTACAGGAGAAAAGAAATTTAGTCATGAA10.4524886877828055No Hit
CGTATATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGGAATGGAGTGGAATGGGAAGGGATGGAATGGAATGGAATGCAATG10.4524886877828055No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAGAGTACATG10.4524886877828055Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGAGGGAAAGAGGGAGGCAGGGAAGAACAGGGAAGCCAGCAGGCATGGA10.4524886877828055No Hit
GAGTGTAGATCTCGGTGGCGCCGTACATTCCCATGTACTCTGCGTTGATA10.4524886877828055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGGGTTATACACC10.4524886877828055No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4524886877828055Clontech Universal Primer Mix Long (95% over 24bp)
GTGTAGGAAAGAGTGTCGATCTCGGTGCGCCTCATCCCATGTACTCTGCG10.4524886877828055No Hit
CATGATCCCAACACAGAAATATAAACTAAATACATAAAATAACCTATTCC10.4524886877828055No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.4524886877828055No Hit
GCGTTGATACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGCCATCGGCCTTCACGGCCGAGACCAGGCCAGCGGCACCGTGGCAA10.4524886877828055No Hit
TCTCGGAGGTCGCCGTTCCCATGTACTCTGCGTTGAAACCACTGCTTCCC10.4524886877828055Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAGCACCTTGACCATCGCCGCTGCGGCCAGCTCTTCCTTGGACGTGA10.4524886877828055No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATC10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGCAACAATGACCTTTGCCCTCTGAATAAATTCACGGTGCTGTGCGA10.4524886877828055No Hit
CGGCCTCCTCGATGTCCTTGGCGGAGTCGCCGCTGGCCAGCGTCCACGCC10.4524886877828055No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACGCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGGCGTCGTGT10.4524886877828055No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATACTCTGCGTTGATACCA10.4524886877828055No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCA10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTGAGTACATGGGAAGCAGTGGT10.4524886877828055Clontech SMART CDS Primer II A (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGCCGACATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGGACTTTT10.4524886877828055Clontech SMART CDS Primer II A (96% over 26bp)
CGCTGGGCGAGCTGGTCGTGGCTGGAGCCGATGTCCACCACGATCTTGGT10.4524886877828055No Hit
AAACTAAATACATAAACTAAGCAAATACTATCTAAAGAGACAAGAACAGA10.4524886877828055No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATACCCCGAGTCT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
ATAACAAATAAGATAACAACAGAACCAGTCTAATAAACACATATAACATC10.4524886877828055No Hit
ATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG10.4524886877828055No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGATCTCGGTGGTCCCGTATCCCATGTACTCTGCGTTGGTACCACT10.4524886877828055Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CGGCAATATCGATAAAACAATAACGACAAAAAATTTACTGAATGTATTAT10.4524886877828055No Hit
ATGGTATACGGGACCCAGATCTACACTCTTTCCCTACACGACGCTGGCCC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 33bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTGAAACA10.4524886877828055No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCT10.4524886877828055Clontech Universal Primer Mix Long (100% over 23bp)
TCGTCGAAATACGCGGAAACTGGAAGTTGGCTCGCGGCCTGCTGCAGTTG10.4524886877828055No Hit
CTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGAGCGTCGTGTAGGGA10.4524886877828055No Hit
ATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCCGAGATCGG10.4524886877828055No Hit
GATCTACACTCTTTCCCAAAACGACGCTCTTCCGATCCTGTCTCTTAAAC10.4524886877828055No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CCCGAGATTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 29bp)
ATACTCAACCCATTGATATCTGGGAGCAGATTAAAGTCCTGATAACCTAA10.4524886877828055No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.4524886877828055Illumina Single End PCR Primer 1 (100% over 30bp)
TCGAGCGTGTCATTGCGGAAGATAAGCTACCGCCGAAGGAAGCGACGCAT10.4524886877828055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCAATGGTTCCAAT10.4524886877828055No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4524886877828055Clontech Universal Primer Mix Long (95% over 22bp)
CTATTACCCAAACAAACTGACTAAACAATTAACATAAACAAATCTCCTAC10.4524886877828055No Hit
AGGGAAGAGTGTAGATCCGGTGGTCCCGTAATCCCATGAACTCTGCGTTG10.4524886877828055No Hit
CCGCGGATCCCACCGCGAACAGCGGTGACGCCGCTCGGTCGAACACCGCG10.4524886877828055No Hit
AGTCTACACTCTTTCCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTAC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.4524886877828055No Hit
GATTACGAGGCTGCGTCAGCGGATTTGGTTGCGGCGGGTTTCGACCTGGC10.4524886877828055No Hit
TTCCCAGCAACTCCAGTCCTTGCATCAGGAGATCGGAAGAGCGTCGTGTA10.4524886877828055Illumina Single End PCR Primer 1 (100% over 21bp)
ATTTTGTATATAATAAGCAAGTTAAATTTTTATCTTCATAATTCTATTTC10.4524886877828055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGATTTTTACAAA10.4524886877828055No Hit
CAAAATACAACCAAAGAAATTACACTCAAACAAACAAAAACAAATAACCA10.4524886877828055No Hit
AAAAGGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10.4524886877828055No Hit
GCGTTATTGCCAGAGAACGTGATCACGGCGACACGATCACGTTTTGCGTA10.4524886877828055No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGACTCTCGTTG10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTGGGGGGGGGGGGGGGGGGCGGGGCGGG10.4524886877828055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTA10.4524886877828055No Hit
GTACAGGGAAGCAGTGGTATCAACGCAAGTACTTTTTTTTTTTTTTTTTT10.4524886877828055No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.4524886877828055Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGTACCT10.4524886877828055Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACAATGGTTACCAAG10.4524886877828055No Hit
CTCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAG10.4524886877828055Illumina Single End PCR Primer 1 (100% over 22bp)
ATTACACTCTTTCCTACACGACGCTCTTCCGATCTCAGCAACTCCAGTCC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 26bp)
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATTCCCTGTCTCTTATA10.4524886877828055Illumina Single End PCR Primer 1 (96% over 32bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGTCGGTAGGGAGATGTAGACTCGGTCCCGACCCCATGT10.4524886877828055No Hit
ATGGTATACGGCGACCACCAGACTACACTGCTCTTCCGATCTGTTCTGGT10.4524886877828055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.4524886877828055No Hit
CATCACCACAATCAAAGGGAATCAAGATGTAGCACAAGAATGCTACCAAG10.4524886877828055No Hit
CGAACCCTTTCGAAGTGTTCCGCTGCTTCCTGTCCGCGACGGCGCCCGTC10.4524886877828055No Hit
GCAGTGAGTGGCAGGGATGCGGCCTCGGCGATAGATAACGATGGCGGGCG10.4524886877828055No Hit
GCTGTGGCCGAGCGCCCGGACGAGGAGTTCTTCGGTCCGCTGCTGCAGGT10.4524886877828055No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.4524886877828055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTACGCAGAGTACTTTTTTTTTTTTTTTTT10.4524886877828055Clontech Universal Primer Mix Long (95% over 22bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCCTGTCTCTTATA10.4524886877828055Clontech SMART CDS Primer II A (100% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTTTGGACGGGGTGTGCATGGTGG10.4524886877828055No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGTTGATAC10.4524886877828055No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCCCCATGTACT10.4524886877828055Clontech SMART CDS Primer II A (95% over 24bp)
TCCCACGCGCGAAGCGCGTCTGGAGTGGGCGTGGCCGGAAGTGACGTAGG10.4524886877828055No Hit
CCCAAGTTCTGCAGGTACCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGA10.4524886877828055Illumina Single End PCR Primer 1 (100% over 29bp)
TCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.4524886877828055Illumina Single End PCR Primer 1 (96% over 31bp)
TACCACTGCTTCCCATGTACTCTGCGGTATCAACGCAGAGTACATGGGAA10.4524886877828055No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATACAC10.4524886877828055Illumina Single End PCR Primer 1 (97% over 35bp)
CTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTTTGGTACCAAAA10.4524886877828055Illumina Single End PCR Primer 1 (100% over 22bp)
ATCTACACACTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC10.4524886877828055Illumina Single End PCR Primer 1 (97% over 37bp)
GATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTC10.4524886877828055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)