FastQCFastQC Report
Tue 10 May 2022
EGAF00003659253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199764
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG5240.26230952523978296No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5230.26180893454276044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5220.261308343845738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG5140.25730361826955805No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4820.24128471596483853Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4190.20974750205242187No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3910.19573096253579225No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3540.17720910674596024No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG3420.1712020183816904No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC3410.1707014276846679No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA3370.16869906489657796No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3270.16369315792635308No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG2870.14366953004545366No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG2850.14266834865140865No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2800.14016539516629622No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT2600.1301535812258465No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC2600.1301535812258465No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2590.129652990528824No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2580.12915239983180152No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA2570.12865180913477903No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT2550.12765062774073407No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT2520.1261488556496666No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT2490.12464708355859914No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC2480.12414649286157665No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2420.12114294867944175No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG2420.12114294867944175No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT2360.11813940449730681No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2330.11663763240623937No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG2330.11663763240623937No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT2310.11563645101219439No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA2290.11463526961814942No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA2270.11363408822410444No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2130.10662581846578963No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2130.10662581846578963No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT2130.10662581846578963No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG2110.10562463707174466No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG2080.10412286498067719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)