FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA725.176132278936017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT624.457225017972681Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG312.2286125089863407Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC231.6534867002156721Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG211.509705248023005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT211.509705248023005Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC191.3659237958303379Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG181.2940330697340043Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC171.2221423436376708Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG151.0783608914450036Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG120.8626887131560028Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT110.7907979870596693Clontech Universal Primer Mix Long (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT110.7907979870596693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA110.7907979870596693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC100.7189072609633357Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG90.6470165348670022Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG80.5751258087706687Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA80.5751258087706687Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC70.503235082674335Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG70.503235082674335Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC70.503235082674335Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.503235082674335No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG60.4313443565780014Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60.4313443565780014Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT60.4313443565780014Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.4313443565780014Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT50.35945363048166784Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT50.35945363048166784Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT50.35945363048166784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA40.28756290438533433Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.28756290438533433Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA40.28756290438533433Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA40.28756290438533433Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.28756290438533433Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAA30.2156721782890007Clontech Universal Primer Mix Long (95% over 21bp)
CGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCTGT30.2156721782890007Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT30.2156721782890007Clontech SMART CDS Primer II A (100% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT30.2156721782890007Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC30.2156721782890007Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2156721782890007Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.2156721782890007Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT30.2156721782890007Clontech SMART CDS Primer II A (96% over 26bp)
TCTATGCACTGATCACATAAGTGATGTAACAGTTGTGTAGGCTCTGCCTA30.2156721782890007No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT30.2156721782890007Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT30.2156721782890007Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACTTGGGAAGCAGTGGATCAACGCAGAGTACATGGGAAGCAGTGGA30.2156721782890007No Hit
GAGGATCACTTGAACCCAGGAGGCAGAGGTTGCAATGAGCCGAGATCGCG30.2156721782890007No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.2156721782890007Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGTGGCGACGGCAGCGAGGCAAAAAAAAAAAAAAAA30.2156721782890007No Hit
CTTATAAACATCTCCGAGCCCACCCCATGTACTCTGCGTTGATACCTGTC30.2156721782890007No Hit
GATTATGTACACGTGAGATGGCCTGGCTGGGCCGGCCGGGCTCACATGGT30.2156721782890007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.2156721782890007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAGATGGATCCCAGTCTGGGTCCTCGCAAG20.14378145219266716No Hit
CCTGTTTAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTAGATCTCGGT20.14378145219266716Illumina Single End PCR Primer 1 (96% over 31bp)
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTTTTTTTTTCAAAGTAAA20.14378145219266716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT20.14378145219266716No Hit
GCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTC20.14378145219266716Clontech SMART CDS Primer II A (96% over 26bp)
CTTCTATACTTCTCCAATACTTGTCTTAGCTTGTTTTTCATTTTCTATCT20.14378145219266716No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.14378145219266716Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGCATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTCGT20.14378145219266716No Hit
TGCCCAGGCTGCAGTGCAATGGCATGATCTCAGCTCACTGCAACCTCCGC20.14378145219266716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA20.14378145219266716No Hit
TACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.14378145219266716Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGACACAGACAGGGGACAAAGACACAGACA20.14378145219266716No Hit
CCACTGCACTCCAGCCTGGATGATAGAATGAGATCCTGTCAAAAAAAAAA20.14378145219266716No Hit
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGACA20.14378145219266716Illumina Single End PCR Primer 1 (100% over 35bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA20.14378145219266716No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
ATTGAGAATAACAAGAATCCAGGCAAAGATCTTAAAAGTTCAATCTTTCC20.14378145219266716No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA20.14378145219266716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
CTTAAAATCTTAAGCTATTAAAATTACGTTAAAAACTTAACGCTAAGCAA20.14378145219266716No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG20.14378145219266716No Hit
GGAGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCGT20.14378145219266716No Hit
GTGCTAAGGGAGTCTCTCTGTCTCTCTGCCTCATTGTGTGTATGCATATT20.14378145219266716No Hit
CTTCACGAGGATGGTGTTGAGGATTTCCGGAGGCAACTTCCCAGCCAGAA20.14378145219266716No Hit
GCAGAGTACATGGGAATCTCCCCGCGGAAAGACGAAATGTTTGTGATGAT20.14378145219266716No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACACAAAAAAAAAAAA20.14378145219266716No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC20.14378145219266716Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.14378145219266716No Hit
TATCAACGCAGAGTACATGGGCCTAAGACGCTCTTCCGATCTCTGGTACC20.14378145219266716No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716No Hit
GGATTGCAGTGGTACAATCATAGCTGTGAGTTTGAACTCTGTCTCTTATA20.14378145219266716No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14378145219266716No Hit
CACTAGACCTCTATTAGGAATCATTTGAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716No Hit
AAATAAAAGGTTACCATAAGTAAGTTCCAGAAAAACAACATATTGCCGAC20.14378145219266716No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATCATACAACAACTGAAGAAGATGTATCAAACAATAATACAAATAACCA20.14378145219266716No Hit
GTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.14378145219266716Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAAAAAAAAA20.14378145219266716Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716No Hit
GAGGCAGAGAGACAGAGATGGAGAGAGACAGAGAGAGGCAGAGAGACAGA20.14378145219266716No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.14378145219266716Clontech Universal Primer Mix Long (96% over 25bp)
TCTCTGTCTCTCTCTGTCTCCCTGCCTCTCTCTGTCTCTGTCTTTCTCTG20.14378145219266716No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTAC20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAGATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAAA20.14378145219266716No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC20.14378145219266716No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATG20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCCAAAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTCTCATGTACTCTGCGTTGATACCACTGCTTCCCA20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
CTTAATTTGTCAGCAATTCAGTCAGCCCTATCTCCAAATGTATCCCTGAC20.14378145219266716No Hit
CTTATACACATCTCCGAGCCCACGAGACGACCGAGTGAGCATCGCGTATG20.14378145219266716No Hit
GTACATGGGCACCCTCTCAGCAACTCCAGTCCTTGCATCATGAGATCGGA20.14378145219266716No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG20.14378145219266716No Hit
GAGAGAGGGAAAGGCAAAGAGAGGCAGAGAGAGAGGCAGGGGGGAAGGCA20.14378145219266716No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT20.14378145219266716No Hit
GTAAAACCTGAAAAACACTTATGGTTTTGCCAGGAAAATGGTTTTAGTAG20.14378145219266716No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
GATAAGAGATATTCCAGGGTTAGTCAAAAGTTAATCTTTGAGTTCCAGAA20.14378145219266716No Hit
CCGAGACACTTTTCCCTACACGACGCTGATCGGAAGAGCGTCGTGTAGGG20.14378145219266716Illumina Single End PCR Primer 1 (95% over 24bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG20.14378145219266716No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716No Hit
CCATGTACTCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.14378145219266716No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTATCAACGCA20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGACGCTCTATGCAAGGACTGGAGTTGCTGGGAAAGAGTGTAG20.14378145219266716No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA20.14378145219266716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14378145219266716No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14378145219266716Clontech Universal Primer Mix Long (95% over 21bp)
CTCTATAAGGGTGCGACATCGCCAGCTAATAATCTACATACGACGCTATC20.14378145219266716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC20.14378145219266716Clontech Universal Primer Mix Long (96% over 26bp)
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC20.14378145219266716Illumina PCR Primer Index 1 (96% over 28bp)
GGTTAACTTTCTTGCTGTGCTTTTTATTATCCCTTGACCAGTTAGCCTCC20.14378145219266716No Hit
ACAAAAAACAAACCCTCCACAAAAATTATCAGGGCATGGTGGTGCAAGTC20.14378145219266716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.14378145219266716No Hit
CTTATACACATCTCCGAGCCCACGAGACGACCGAGTGAGCATCTCGTATG20.14378145219266716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)