FastQCFastQC Report
Tue 10 May 2022
EGAF00003659381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319090
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5180.16233664483374596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4870.15262151744022062Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3860.1209690056097026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3770.11814848475351782Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3290.10310570685386569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)