FastQCFastQC Report
Tue 10 May 2022
EGAF00003659423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211548
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA8370.39565488683419364No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA7880.3724922948928848No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG7440.3516932327415055No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG6940.32805793484221074No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCT5950.2812600450016072No Hit
GTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAG5470.2585701590182843No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC4880.2306805074971165No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT4700.22217180025337038No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4250.20090003214400515No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT4170.197118384480118No Hit
TATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTC4140.19570026660616033No Hit
ACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTG4070.19239132490025904No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG3960.18719155936241422No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG3890.18388261765651295No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT3810.1801009699926258No Hit
GCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATTAGCCCGA3810.1801009699926258No Hit
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT3670.17348308658082326No Hit
GAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAAC3640.17206496870686558No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC3410.16119273167319No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC3310.15646567209333107No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT3280.15504755421937338No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG3280.15504755421937338No Hit
TAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTAT3190.15079320059750032No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC3150.14890237676555676No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG3140.14842967080757086No Hit
AGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCC3130.14795696484958498No Hit
GTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTG3110.14701155293361318No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT3100.14653884697562727No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3070.1451207291016696No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3050.14417531718569782No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT3050.14417531718569782No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT3030.143229905269726No Hit
GTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT3010.14228449335375423No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2920.13803013973188116No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG2840.13424849206799402No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG2820.13330308015202225No Hit
TGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCT2750.12999413844612098No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2750.12999413844612098No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG2740.12952143248813508No Hit
CTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCT2740.12952143248813508No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC2690.1271579026982056No Hit
GTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTC2620.12384896099230434No Hit
AGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGT2570.12148543120237487No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA2460.11628566566453002No Hit
CATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAG2430.11486754779057234No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG2430.11486754779057234No Hit
CTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGA2400.11344942991661468No Hit
TCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA2370.112031312042657No Hit
TCAACAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTCTCT2350.11108590012668519No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2300.10872237033675573No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAA2290.10824966437876983No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2290.10824966437876983No Hit
CTCCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGA2260.10683154650481215No Hit
CTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAA2260.10683154650481215No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT2250.10635884054682626No Hit
ATTTGAAGGAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACA2250.10635884054682626No Hit
ACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTC2250.10635884054682626No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2210.10446801671488268No Hit
CTCTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCAACCTCC2170.1025771928829391No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC2150.1016317809669673No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA2150.1016317809669673No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2140.10115907500898141No Hit
ACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2130.10068636905099553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)