FastQCFastQC Report
Wed 8 May 2024
EGAF00003659424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211548
Total Bases29.6 Mbp
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA7910.37391041276684256No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA7520.3554748804053926No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG7300.34507534932970296No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG6590.31151322631270445No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCT5550.26235180668217145No Hit
GTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAG4840.2287896836651729No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT4780.22595344791725755No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG4230.19995462022803337No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC4220.19948191427004747No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC4090.19333673681623081No Hit
TATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTC4070.19239132490025904No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT3810.1801009699926258No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC3740.17679202828672452No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT3560.1682833210429784No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT3500.16544708529506305No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG3350.15835649592527465No Hit
ACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTG3340.15788378996728875No Hit
GCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATTAGCCCGA3320.15693837805131697No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3290.15552026017735926No Hit
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT3280.15504755421937338No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG3230.1526840244294439No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC3090.1460661410176414No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG3040.1437026112277119No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC3030.143229905269726No Hit
AGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCC3010.14228449335375423No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG3000.14181178739576833No Hit
GTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTG2990.14133908143778243No Hit
TAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTAT2970.14039366952181065No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2950.13944825760583884No Hit
GAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAAC2930.13850284568986707No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG2910.1375574337738953No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT2890.1366120218579235No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG2810.13283037419403634No Hit
GTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT2770.13093955036209276No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC2770.13093955036209276No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT2720.12857602057216327No Hit
CTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCT2710.1281033146141774No Hit
TGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCT2610.12337625503431844No Hit
CATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAG2610.12337625503431844No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2580.12195813716036076No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT2540.12006731332841719No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG2520.11912190141244541No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG2480.11723107758050183No Hit
CTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAA2420.11439484183258646No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA2390.11297672395862877No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC2350.11108590012668519No Hit
ACCATGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGCAGC2340.11061319416869929No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAA2310.10919507629474161No Hit
GCTTTATTTCCATTTGGTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGC2210.10446801671488268No Hit
TCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA2200.10399531075689677No Hit
TCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGC2190.10352260479891087No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2190.10352260479891087No Hit
AGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGT2180.103049898840925No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC2160.10210448692495319No Hit
CTTCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGAGCCACTTCT2150.1016317809669673No Hit
GTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGG2150.1016317809669673No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT2140.10115907500898141No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2120.10021366309300962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)