FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG100.28868360277136257No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC90.25981524249422633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70.20207852193995382Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA70.20207852193995382No Hit
GGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGC70.20207852193995382No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60.17321016166281755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC50.14434180138568128No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA50.14434180138568128No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA50.14434180138568128No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC50.14434180138568128No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG50.14434180138568128No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG50.14434180138568128No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC40.11547344110854503No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC40.11547344110854503No Hit
GTTCCAGGTCATACACGTACTCCTGGACCCAGGATTCACTGGGATCAGCA40.11547344110854503No Hit
GTCCAGAGGATGTATGGCTGCGACGTGGGGTCGGACTGGCGCTTCCTCCG40.11547344110854503No Hit
AATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG40.11547344110854503No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG40.11547344110854503No Hit
GGACACAGCTGGGTTCTGAAGCTTCTGAGTTCTGCAGCCTCACCTCTGAG40.11547344110854503No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG40.11547344110854503No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT40.11547344110854503No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.11547344110854503Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTCACACCATCCAGAGGATGTATGGCTGCGACGTGGGGCCGGACGGG40.11547344110854503No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG40.11547344110854503No Hit
CGTTAGTTCCTCTGTTACATTCAGCCGATCATCGGAGTAGAATAAGTTAG40.11547344110854503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)