FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG80.23094688221709006No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60.17321016166281755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT60.17321016166281755No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT50.14434180138568128No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC50.14434180138568128No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA50.14434180138568128No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT50.14434180138568128No Hit
GCTCTCCACTGCTCCGCCTCACGGGCCGCCTCCCACTTGCGCTGGGTGAT50.14434180138568128No Hit
GTTCTCACACCATCCAGAGGATGTATGGCTGCGACGTGGGGCCGGACGGG50.14434180138568128No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC50.14434180138568128No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC50.14434180138568128No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG40.11547344110854503No Hit
ATCCTCAATAGCCACTGCTTCGCCAGAGAGAAGGAAGGGGATAAACCCAG40.11547344110854503No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA40.11547344110854503No Hit
GAACAGAGATGGTTTCATCGACAAGGAAGATTTGCATGATATGCTTGCTT40.11547344110854503No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.11547344110854503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG40.11547344110854503No Hit
CAGCACCTCAGGGTGGCCTCATGGTCAGAGACAGCGTGGTGAGTCATATG40.11547344110854503No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG40.11547344110854503No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG40.11547344110854503No Hit
GTCCTGTCTCTCCTCATGCTAGTAGCTGCCTTCTGCTCTCCAGCGCTCTC40.11547344110854503No Hit
GGTCTCCACGAGCTCCGTGTCCTGGGTCTGGTCCTCCCCATCCCGCTGCC40.11547344110854503No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.11547344110854503Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)