FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38.333333333333332Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGTGCCGGAAGTACCCCATTGAGACGGTGAGCCCATCGCCTACCCGTC38.333333333333332No Hit
GTGGAAACACCCGTGATGGTTCGACTCCATTCAGCCGCATCGGAAACCCC25.555555555555555No Hit
ACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT25.555555555555555No Hit
GTATATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25.555555555555555Clontech Universal Primer Mix Long (96% over 25bp)
AAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCC25.555555555555555No Hit
GGCCAATTGCTCGACCCGCTTAAAGAGCGCATTCAGTCTTTCGAAAAGCG12.7777777777777777No Hit
AGGCGTATCTGGCGCATATTTCTCGAGTAACCAGGCTGCTGGCATTTGCA12.7777777777777777No Hit
TGCCAATGGTCACCTGCCACACTCGCACCAGGTTGTCCGTGTAGCCAGCA12.7777777777777777No Hit
GGACGTGAAGACCGAGCTCACCACCCCCGAGGCGCCCGACCTGCACGGCC12.7777777777777777No Hit
GTGCAGGCGCGCTGGCGCAGTACATCGGGCGGCAGGATCTCGCCGTCGGC12.7777777777777777No Hit
CCATTGAACTGTACACTTAAAAATGGTTAAGATGGTATATTTTGTGTTTT12.7777777777777777No Hit
TTCTTAATATTCATGAAATCTGTGACTGAATACTAGGACTTCAACAAATA12.7777777777777777No Hit
CCCCGCAACTCCTTCGCGAGCGCCTGGGCGGTCGGCTTCAGTGCGCCGGT12.7777777777777777No Hit
TCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATC12.7777777777777777No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAACTCTCTAATAAACAA12.7777777777777777No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
TCTTCATATTATGCTAGACAGAATAATTCTCAGTAACTTCCTTGTGTTGT12.7777777777777777No Hit
AAACAAACCAATCCCCCTCTTCCCATCCTGTGAGGAGAAAAAAAATACTT12.7777777777777777No Hit
GGCTAGGAGTCCCAGGGACAGCAGCCTCTGCTCAGGGCAGACTCTGTGAT12.7777777777777777No Hit
CCCTCAGGGTCCCCGCTGCCTCTGCCCCACTGGCTACACCGGAGGCAGCT12.7777777777777777No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT12.7777777777777777No Hit
TTCTACCACATGTATCAGCATTGGCTTGCCAACAATATCGCGCAGCACTT12.7777777777777777No Hit
CTTCTAGGCTCCATTCTGCAGCATCTGTTGTGGATTCCAAAATATCTTCT12.7777777777777777No Hit
AAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACC12.7777777777777777No Hit
GAGCTGGAGAAGGAGCTGTACCGAGAGGCTCGGGAGTGTCTGACCCTGCT12.7777777777777777No Hit
AAATGGACCCCAACTGCTCTTGCGCCACTGGTGGCTCCTGCACGTGCGCC12.7777777777777777No Hit
CCCCTCGGTGTTGCGGTCACCGCCGAATTTCTGGGGCCATTGGGCATGGC12.7777777777777777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)