FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC46.349206349206349Clontech Universal Primer Mix Long (96% over 26bp)
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA46.349206349206349No Hit
CTAACAAGGAGGCAAACAAGACAAAAGGCAAAGAAAGTACCAGACTGAGG23.1746031746031744No Hit
CATTACTTGGTCTCAAACATCTCCCATGACACGGTCCTCCAATGCCACTT23.1746031746031744No Hit
ACTTAAAGCACCTTCACATAGGATCAGCGTGTCAGCATCCAACAAATGAT23.1746031746031744No Hit
GGCAGGCAGACCCCAAGGAGCCATCACGGGCAAGGGCTGGAGTTTGATTA23.1746031746031744No Hit
GGACATTTCTTTTTTGTGAAAGAAGGTGAAAAACCAGAACCTGAGCCCGA23.1746031746031744No Hit
CTCCTAAAGGAGTATTGGTTGTTGGTGGTCCGGAAGAGGTTGCGGAAAAA11.5873015873015872No Hit
GCATGGAATCATCATCAAATGGAATCGAATGGAATCATCATCAAATGGAA11.5873015873015872No Hit
GTCCATCAACAAGAGAGTGAATAAACACAGTGAGAACTATCCATGCAGTG11.5873015873015872No Hit
ATCACAAGAATAGCAAAGGGGAAGTCCACCCCCATGATCCAGTCACCTCC11.5873015873015872No Hit
GCCATAGTCCCGCAGCAAGAGCGGCAACCTGAGGACTGGTAAAAGATGTA11.5873015873015872No Hit
CGCGTTCACCCACCAGCGAGTCGTAGCCGTGCGGCAGGCGCAGGATGAAC11.5873015873015872No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.5873015873015872Clontech Universal Primer Mix Long (100% over 23bp)
ATTCTATTCGATTGCATTCGATAATTCCATTCGATTCCATTTAATGATAA11.5873015873015872No Hit
GGCCGGCGGCATCGTTGAAGTCAACGCCGCGCCGGGTTTGCGCATGCATT11.5873015873015872No Hit
GCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCA11.5873015873015872No Hit
GTGACGTACAGACTGGCGGGCCCACCGGATGCCTTTCGGCTGGGGAGGCA11.5873015873015872No Hit
CATCATCAAAGTTCTTTTTGTTCCTTTTGGAAATGTAGCTGGCTTTATAT11.5873015873015872No Hit
GATTACAGGTGTGAGCCACCGCACTCAGCTGTGGAATTTTCGGAAAACCA11.5873015873015872No Hit
TAAGTATGCTGTGCGCTTCATCCCTCGGGAGAATGACGTTTACCTGATTG11.5873015873015872No Hit
GTATGAGTTCCTCACTCCAGTTGAGGAGGCTCCCAAGGGCATGCTGGCCC11.5873015873015872No Hit
TTATTTTATTGTCCAAAGAGAGTCAGAGCTAAGCATACAGGCTTGGGGGT11.5873015873015872No Hit
GGACTTCCATTGTATCTGCATAATGCCGAGACCAGCTCAGTCTGGGAGAC11.5873015873015872No Hit
CAATTGTCATTCATTAATTTATTGTTGCCCTACTATGTGTGGCAGGAAGC11.5873015873015872No Hit
GCATGTTGTGCACATGTACCCTAGAACTTGAAGTACAATTAAAAAAAAAA11.5873015873015872No Hit
ACGAGATCCAGCACGGGGACCTGAAGCTGCTTCGGGAGATTGAAAAGGCC11.5873015873015872No Hit
GAAAGAAAGAAAGGATCAGTAGAGCTATGGTGGTCGTGAGTCATTCCCCG11.5873015873015872No Hit
AAATCTATCTTACTGTGGTTCTCCATGGGCAGCAGGACCATTTTTCAGAA11.5873015873015872No Hit
AGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGA11.5873015873015872No Hit
AGCCAGGTCAGGCGAGAGATGCCGCTGAATGCAGGGAAGCCAAACCGCTC11.5873015873015872No Hit
CAGGAGGCTGTTCTGAGGATCACAGGGTCCAATCTTGGAGCCCAGGTGTT11.5873015873015872No Hit
CCTTACAACTTCCAGAGAGAAAATACAAAACAAGAAACAGACTTGGTTTC11.5873015873015872No Hit
ATGGTGTCTTGGAGGCAAACTACAGTTTGCTGTAAGATAACTTTCCGTGC11.5873015873015872No Hit
GGTTACATCCGGCCCGTAGGCTTCATTCATAGCTTCTGCGTAATTAAGCA11.5873015873015872No Hit
GGCAGTCTAGTCCCCAGGCAAGAAGGTGGTTTGTTTGGGGAAGAGGCTGT11.5873015873015872No Hit
CCAATATCCAGCTGATCTGATCGAAAAACAGTTACTTAAGCTTAAAGAAG11.5873015873015872No Hit
ACATTTGAGATGGGTACATACCTCCCAGAGCCAGACTTGGGAGGAATCTG11.5873015873015872No Hit
AACATGAATCATTCAAGTAAAACCAACTTAACTATAGAAATGTAGCAAAG11.5873015873015872No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT11.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
ATATAGAAGCAGCCTATTGTATTTGCGGAACGGAGCTGGTCAGCAATTTT11.5873015873015872No Hit
CTTCTCACCTAGTGTGACAAGAACATGCCGGATTTCAGCACCCATGACGG11.5873015873015872No Hit
ACATAGAAGCCCTCAAAGGAAAATATACCATCGTGATCATCGCCCATAGA11.5873015873015872No Hit
AGACAAAGATATATCTGAGGTCTTACTATCCAACAGTCTTCCTTTTTCGC11.5873015873015872No Hit
CATACACCACACACACAAACTAACACACACACACATAAAATTTATGGCAT11.5873015873015872No Hit
GTGTTGATCTGCTTTCTGTCACTGCTTTCTAGTTTGTATTTTCTAGTTTT11.5873015873015872No Hit
GGGTCGGGTTACCCCCAAAGGGAAGCCCATCAGACTAACAACAGATCTCT11.5873015873015872No Hit
GGTCTGAAAAAATAATCCGTTTAATTGAAAAACCTGGAGGATACTATTCC11.5873015873015872No Hit
GACATAGAGAAGGCTGAAGCAGGAGGGGAGGATGAGACTCTTAGTGAGGA11.5873015873015872No Hit
GAACGGTTGTCCCAGCTAACTGAACTGGGTCCATGATAGCGGTTGGCGTA11.5873015873015872No Hit
GCTTCGTCTCGCAGAGCCGGGGACAAGAGAAGTCGAAGATGGACTGCCAT11.5873015873015872No Hit
ATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAA11.5873015873015872No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)