FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA46.349206349206349Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA46.349206349206349No Hit
AATGGCAGACCTGTCGTTGACGAATAAGAAGACCTTGCTTGTGCTTCCAG23.1746031746031744No Hit
CTTTGAGACCAGTGCCAAGGAGACCATCAACGTGGAGCAGGCGTTCCAGA23.1746031746031744No Hit
CCGCTTCAATGATAACTACTTGCTTCTGTTGATCGGCTTCGCTAAAACTA23.1746031746031744No Hit
GAACAGGGACAGCAACACCGACACCACCGTGACTATGATGACCATCTGGC23.1746031746031744No Hit
TTCCACCTCTGTTTCACCAAATTTAGTGACCGTTTTCATACATTGGGGCT23.1746031746031744No Hit
GGTTATTACCTCAGAAAATATGTAGATGAAGGGCTGGACCTGTTGCAAAA11.5873015873015872No Hit
CAGCAGCACGTTGCGCGCGCTGCGCGGGCCGCTGCAGTCGCCGGTGTCGA11.5873015873015872No Hit
CCATTCAATTCTGTTCAATGATTCCATTAGATTCCATTTGATGATGATTC11.5873015873015872No Hit
GGCAAGATCCTGTACAGCCAGTGTGGGGATGTGATGAGGGCCCTGGGCCA11.5873015873015872No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGCGTTTTTA11.5873015873015872No Hit
GGTGTGCACCCAGTCACCCCCACTGGATTATGGTGCTGGTAGCATGAGAG11.5873015873015872No Hit
ATTTGTAATAGCCCAACCCTGGAAATAACCCAAATGCTCATCAGCAGGTG11.5873015873015872No Hit
CCTTATGGCTACCCATAATTACGAATTGTTGAAAAAATACCCCTACCGGG11.5873015873015872No Hit
AGACGGGGGCCTGTGACTTTGGCCTTGAAGGGGCTGCCCCCAATGTGGTA11.5873015873015872No Hit
CCCTAGGCCCTTTGCCCTTTGCACTTTGCCCTTTGTGAACTGATTTGATT11.5873015873015872No Hit
ACTTCCGGCAGCAGATGGAGAATTACCCGAAAAACAACCACACTGCTTCG11.5873015873015872No Hit
GCCTGCTGCTGCCCCCTTCTGGGACCCTAATTTTCTGGACTTTGAGAAAT11.5873015873015872No Hit
ATTAATTGATGCAGTTTATTCATAGCATCAATAGTCTTTACAATTTGGCA11.5873015873015872No Hit
CATCATCAGATGGAAATGAATGGAATCGTCATAGAATGGAATCGAATGGA11.5873015873015872No Hit
CATATAAACTACATCATACTCAGAATGTGATATAAGTTCTGGAGGCCTTG11.5873015873015872No Hit
GGCATATATTAGTTCTCTTCCAATTCCTCTCCTTTCCTCTGACCCTGTGT11.5873015873015872No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT11.5873015873015872Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCGCGCCCTTTGCCTGCCCGCCGAGAGGCAGGCGTGAAATCCAAATT11.5873015873015872No Hit
TCCCTAGGCTACACCCAGGGAACTGGGAACAGCCAGGTGCCCCAAAGCAA11.5873015873015872No Hit
GTGCAAGCTCTTCCTGTAGTGCATTCAGTTCAATGTACTGGCCCGTCAGG11.5873015873015872No Hit
CTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGC11.5873015873015872No Hit
CTGCAGGATGGGCCAAAGGATTTCGGTGGAATCCCAAAACTCGCAGCGCG11.5873015873015872No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC11.5873015873015872No Hit
GTTATTACCCGAGGAGCTTCCATGGCAGCCAAAAAGGGCATTTTGGTGGT11.5873015873015872No Hit
GTAAATGAGTTTGAAGTTCAAGAAAAAGTTGGGTTTACAGTAAAAGCTCC11.5873015873015872No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC11.5873015873015872No Hit
GAGTCGGAGCTCGGCAGGAAGTGGGACCGGTGTCTGGCGGATGCGGTCGT11.5873015873015872No Hit
GAAATATAGAGGTGTGAAGTGGGAAATCAGGGTAAAACTCTGTTCGGGGC11.5873015873015872No Hit
ATTCCTACAAAGAAAAACAGACAGTCGACTTCTCCCAACTGACAGCTGCG11.5873015873015872No Hit
TCTCTGTCTCACCCGGGCAACCACTGCCCTGCTTTTGGGAACTATAGATT11.5873015873015872No Hit
GATGAATGCAGAGCCCGAGAGGAAGTTTGGCGTGGTGGTGGTTGGTGTTG11.5873015873015872No Hit
CTCATTGGGCCAGCAACAACCACAAAAGAATCTAGGCATTAGAATGAACT11.5873015873015872No Hit
CCATTACTCTGCATTGGATTTCCCGCCCAAGGGTCTGCCCACCATGCAAT11.5873015873015872No Hit
GTTATGGCTGGGTGGTTCAGGGGTTTTTTTTGGGTTTCTTTTTTTTTTTT11.5873015873015872No Hit
GCGCTGCCACGTCTGGCTGATATCGTATTTTTAGTGGAGACGGGGCTTCT11.5873015873015872No Hit
CGGGTGGGATGCATCAGAGGTTGTGTTCCATAGTGCCTTCCAGAATTCTG11.5873015873015872No Hit
AATGGCATAGACGCTTTCCTTCAAGTTTCTGTTCCATTCCATGGCGTTCA11.5873015873015872No Hit
CTCCATGTGGCATTCAGGCACCCTTGCCTCGGCCAAACAAGCGGCCCTGT11.5873015873015872No Hit
TAACCATCTCGGCAGCCAGTCACAATTTCAGGTGCTCCTTCTCCAATTCC11.5873015873015872No Hit
CTCCTCAGCCTCTCAAAGTGCTGGGATTACGGGCATAAGCCACCATGCTT11.5873015873015872No Hit
GTGTAGCCTTATGTGGCTAATAGAATGAGCACTAGATTTCAGCTGCTTCC11.5873015873015872No Hit
GGCTGGGTCTCTGGCCTCCGCTGGCTTCCTGGCACTGTTCTCTGAGTATG11.5873015873015872No Hit
GGGCGCGATCGAACTGCGCGACCTGGGCTTCCGATATGGCAACCGCGCGG11.5873015873015872No Hit
GCCCTGAGGAGGTGTTACAAGCAGAGGCGTGCAGCACCACGGAGGATGGG11.5873015873015872No Hit
TAGCAAAGCCTCTATACCAATTGCTTAATAAGCGGGATAACTTTCGCACC11.5873015873015872No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)