FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA612.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGAGGCAGGAGGATCACTGGAGGCCAGGAGTTTAAGGCTGCAGTGGG24.0No Hit
GGTCCTGCCTCTGGCGTGGAGCCCACAAGTTTCCCAGTCGAGTCTGAGGC24.0No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT24.0Clontech Universal Primer Mix Long (100% over 23bp)
GGCACCCACAAGCCGGCCGAGGCCGTGCACGTGCAGATTGACGAGCCAAT12.0No Hit
TTTTTATACTATTTCACACAATTTAAAATAATCAACATGAAAAGGTCAAC12.0No Hit
GTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTCTCGCGGTTGGCCTT12.0No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC12.0No Hit
CAGCCGCGGCACGTTGGGCAGGAAGCGCGCGCTGCCGATGGTGGCGCGCA12.0No Hit
ATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGT12.0No Hit
GAAAGACGCTGACGTGTCGTCACGAATTGCGGATGCTGCGCAAGCTGTCT12.0No Hit
GCCCCAGACCATGCGTTCGTGGTACGTGGGCGTGATCTTGCGGCGCTTGA12.0No Hit
AATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCT12.0No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12.0Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGGCGCGGTCTCGAATTGCCTTGGTGCGCGAGAGCTCGTCGAGCTTG12.0No Hit
CTTGGAGGGTTTCTTCGGGAAGATCTTCGACCTCTGGGAGGCCAAGTTCT12.0No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA12.0No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATTTTCTGTTGCTGTGTAACAAATTACCATAAACTTAGCTGTGGCTCAG12.0No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAA12.0Clontech Universal Primer Mix Long (96% over 26bp)
ATTCCACATATGTTAATAAAGAACTTGATGCTGGATACTGTCCTAAGCCC12.0No Hit
CTTCTGCGGCCGATGAGAAGAAGAAGGGGCCCAAAGTCACCGTCAAGGTG12.0No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12.0Clontech Universal Primer Mix Long (96% over 26bp)
GAGCTAGCTGTTCTAGTATTTGCTTTTTGTAATCCAGTTAAGACCATGAG12.0No Hit
CTTGAACTCGGGGCCGCCGCGGATCACGGGAAAAAGCAGCGGCGGGATCT12.0No Hit
GAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCC12.0No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.0Clontech Universal Primer Mix Long (96% over 26bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGGTATCAACGCAGAGTACATGGGAAGCAGT12.0No Hit
GGATTTTTAGAGTTTATTAAGCAGGGGAGTGGAGGGGAGATGTGGCACAA12.0No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.0Clontech Universal Primer Mix Long (96% over 26bp)
GATCGCTGCTGGCACGTAAGTCGCCCGAACTCGTCGTGGCTGTTGAGCTG12.0No Hit
GTTTATTTCCTTTCAACCTTTTCAAGTACACTGGAACATCACTTTTAATG12.0No Hit
CTGCATGTCTGCCCTGGCTTCACCTGGGCCTTGTTGCTAGGTAGCCTGAG12.0No Hit
TAATAACACAAGCCAAACAAAAACATATCTGGGGATGAATCTGCGAAACC12.0No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC12.0Clontech Universal Primer Mix Long (96% over 25bp)
CCTCTGGTCTGTGCCACTTTGATCCTGCTGCTCCTTGGCCTCTCTGGCCT12.0No Hit
ACTGGGCACAGCGCTCAGACAGCCCATCCAACCCTTGGGTGGTAGTCTCG12.0No Hit
GTGTGGGCCGAATCAACCCATGCCGACGACCTGGATCGTCACTCCTTTAC12.0No Hit
TGGGTGAGCTACATGCCTGTCTGAGCATTCTCGACATGCCGTTGCTCGAG12.0No Hit
GTTTTAAACAGGGTCGTCTTACCGACGCCGTTTGGGCCGATAACACCGAC12.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)