FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length140
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC36.0Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC36.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCACATGGTCAACCATGAGGGAGCGGTGGATGTCATCTGCTGCGTCCCA24.0No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT24.0Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTCAACTGTCAAATGAGTTAGTGATAACTTCTCAGTTGAAAGCTCAC24.0No Hit
CCTCTGGAAGCTTTGAGTAAAACGACATCGTGTGAGGTCCAACCTAGTGA12.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTTCCCATGTACT12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCGCAGCATCCGCAATTCGTGACGACACGTCAGCGTCTTTCCTGTCT12.0No Hit
GATCTGTGAGGTCGATCGCGGCCAGTTGCACCCCTACGAGGGCAACTACT12.0No Hit
GCATACGTCCTTTAACTTCGAGTTCCTCATCTCGGCGTAGAAGGCCAACT12.0No Hit
GAGTGCTGGGATTATAGGCATGAGCCACTGTTCCTGGCTGTGAGGAGGAA12.0No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.0Clontech Universal Primer Mix Long (96% over 26bp)
CAGGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGAT12.0No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTGCGCGGGGGGGATGCGTG12.0No Hit
CGCCAAGTTCCCATATAACAGGTGCAGGGCATGCTTCATTTACCATTGAA12.0No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGC12.0Clontech Universal Primer Mix Long (96% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA12.0Clontech Universal Primer Mix Long (96% over 26bp)
GGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCC12.0No Hit
GCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGT12.0No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT12.0No Hit
CGTCTATACCGGCGTGGAATGGCTTCCCAACGCGCGCCTGGCCTGGAAGC12.0No Hit
ACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACAGCTG12.0No Hit
TCGCATGGTCGACCGGCGCCCTGGCCCAGAAGTCCGCCAATGGCCGCAGC12.0No Hit
GAGAAGATGCAACCAATCCTGCTTCTGCTGGCCTTCCTCCTGCTGCCCAG12.0No Hit
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT12.0Clontech SMART CDS Primer II A (96% over 26bp)
ATCGTGGGGCGCCCCAGGCACCAGGGCGTGATGGTGGGCATGGGTCAGAA12.0No Hit
GGCGCTGTTGATGCCTCGCAAACTTGCGTTATCGGCCTCCAGCTCCGCGA12.0No Hit
CCTCCATGCCCTCTAGAACTTTGCCAAACACCACATGCTTGCCATCTAGC12.0No Hit
GTCCACGGACTCTCCGGTATTTTAGGAGGTCCCTGGCCACAGATTTATAT12.0No Hit
GAAGCACATAGCAGTGACACAGCAGAGAAGGCCACAGAAGAGAAGCAGGA12.0No Hit
CTTCAGCTTCGTGCTGCCGCCGGTGCAGCAGGGCGCGCCGCACCTGCAGC12.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGGACATGGGAAGCAGTGGTA12.0Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATCAACGCAGTGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGGGATCACGGATCATGAGTCCGTCCTCGGTCTGCTCGAACAGCAGTGC12.0No Hit
CTAGTGAAATTGTCTAAAGAAACTAAAGGGCAGTCATGCCCTGTTTGCAG12.0No Hit
CTGCTATGAAGTCTCGACCCTAAGCCCAGGGTCTGGCCTTGAAAGCTCCG12.0No Hit
ATCCATGATCGGATCGCCAGTTTCGCTCTACGAGAGCCGAACGGATAAAA12.0No Hit
ACATCTTTGATGATCCCCTGAATGTCTTTGGAGGCCAGTAGAGCACACAG12.0No Hit
ACCAGCTGCAGTTGGTACCGAAGATGCTGCGGATGCTGCGCCCCGACCTG12.0No Hit
AATATACCATATCTTATTCCCAGTGTGGCAGGTAGACTCTAAAGTGTACC12.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)