FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTAGGAAAAATGGAGAAGGTTTACTCTTTCTTCCATAATTTCTCCTGC46.896551724137931No Hit
GCCTTAGAGGATGATGCTCATTCAGCCCGGGACCAGGAATGAGAATAGAA35.172413793103448No Hit
GGCGTGAGCCACTGTGCCCGGCCTGTACCCCATAGGTTTTGGTTTGACTT23.4482758620689653No Hit
ATACAGTACTTTGGATAACTTCTTCCATCAGTTTAGTGCAATGTATCATT23.4482758620689653No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG23.4482758620689653No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG23.4482758620689653No Hit
GCTGATCTTCTTGGCGCCCGCATAGGTGAGCGTCGAGAAACGCGGGCGGT23.4482758620689653No Hit
TTTCCTTTCCTTAACAGTAGCCATTTGAAAACAAAGTATTAGTAAAATTA23.4482758620689653No Hit
GATTGGAATCGAATGGAATCATTGAATGGACTCCAATGGAATCATCACCA23.4482758620689653No Hit
TAATATGCCTGCTATTTGAAGTGTAATTGAGAAGGAAAATTTTAGGGTGC11.7241379310344827No Hit
GTCAGGAATTGTTCCTCTTCCCATCAGCCAGAGGAGAAAGCCTTCATACA11.7241379310344827No Hit
GCACCACATCTTAGCACAGGCTCCAAGAACTACTAATGGTTATGTCTCAG11.7241379310344827No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG11.7241379310344827No Hit
GATCTAAGGAGCAATGGAAAGAACTGAGTCAGGAGGCCTGGGGCAAAGGT11.7241379310344827No Hit
GTGTTGGCCAGGATGGTCTCAATCTCCTGACCTTGTGATCCGCCCGCCTT11.7241379310344827No Hit
GGTAAACAGGGTCAAGGCAATTGCCGCTGCCAGCCAGGGCGATATGTCCA11.7241379310344827No Hit
CCTTTACTCGTATCTAAGCCGTGCTCGCACAGCGCGCAATGCTCTGGCAC11.7241379310344827No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG11.7241379310344827No Hit
GAATGAGATCCTGTCATTTGCAACAAACATGGGAGTGCAGACATCTCTTT11.7241379310344827No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCG11.7241379310344827Clontech Universal Primer Mix Long (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTGATAGTTGAGAAAACAGAGTCC11.7241379310344827No Hit
GGGCAGGGAGGTGTTGGACAAGCACTGCACAGCTGTGGGATGAGACACCC11.7241379310344827No Hit
AAAATGGAAAGCATAAGAGTCATGCACAGAGGTCACCTCGAAATGATGCA11.7241379310344827No Hit
TAATATGCCTGCTATTTGAAGTGTAATTGAGAAGGAAAATTTTAGCGTGC11.7241379310344827No Hit
CCCCAGCTGACCAGGACTGTGAAGATGCGGTTCCGCTGCGAAGATGGGGA11.7241379310344827No Hit
AAACTAGATTCTTACCACAACTATCCTATAAACACTGCAACTATTCTTTC11.7241379310344827No Hit
GCACTTTAGGATATTAGATGTTGGTAGCTCAGGGTTGTGTCTTTCAAATT11.7241379310344827No Hit
CGCCGGGGAAAGCCGAGCCGAGGCACCCACCGACCGGAGCCCAAGCGACA11.7241379310344827No Hit
GAATTGTGGGTGCCGCCACGTTTTCCTGAACGTTCGGTCAGCGGGGTGTT11.7241379310344827No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGCTATTTCTGACTAATT11.7241379310344827No Hit
CAGCAGCCTAGCCAGTCGGATTTGATGCTTCCAAACTTCACGCTCTTCAG11.7241379310344827No Hit
AGAAATAAAATCCCATGCATTATAATGAAGAGTGTTTTAATTTTGTTTTT11.7241379310344827No Hit
CTCATAAGCACTGCTTTGCTGGATGATTTTCACTGCTTTGTGTTAATGAC11.7241379310344827No Hit
AAATGTAATCACACTAACATGATGCCAGAAACAACTGATTCATCTACATG11.7241379310344827No Hit
CTGTTTAGGGAAATACCTTGATAAGCTGTTGGAGGGTGGAAGGGAGCAGC11.7241379310344827No Hit
TGCCCAAGGTGGTTGGAGCACAGCTTGGCTTTAGACATTTTAGGGAGACA11.7241379310344827No Hit
GTCTTGTAGTCGTTGCTGCCGAAAAAGTCGCGGTTGAAGGTGACGTAGTT11.7241379310344827No Hit
AATTAGAGCTGTCTTACCTTGAATGTTCATGACTAAAACCTCCAAACTTA11.7241379310344827No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11.7241379310344827Clontech Universal Primer Mix Long (96% over 25bp)
TTGACAAACCATCCCAGATGCAAGTGACCGATGTTCAGGACAACAGCATT11.7241379310344827No Hit
TTAGATGATTCCATTCGGGTCCATTCGATGATTCTCTTCGATTCCATTCG11.7241379310344827No Hit
TTCCGTGGCTGGGCGTCGTATTTCCCTTTGAATTTAGAGCACTTCTCCTT11.7241379310344827No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT11.7241379310344827No Hit
CATCAGACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATT11.7241379310344827No Hit
CGCCAGCTCATGGTGCCCCGCCTGCCTGGCATAGTCAATGGGTGTGCGGT11.7241379310344827No Hit
ACATGGGAGTGGACTGGAATGGAGTGGAATGGAATAGAGTGGAATGGAAT11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)