FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCACTTTATTCAAATTGTTTAAATATTTGCTGGGTCATTGTTCATAG46.896551724137931No Hit
GATTACAGGCATGAGCCACTGCACCCAGCCTAGTTCTACATTTTCTTGTC35.172413793103448No Hit
ATGCAAGATCATGTCATCTGTACATAAAGACAACTGTACTTCTTCCTTTG23.4482758620689653No Hit
GAGAAAGAGTGCCCTGCCCCACACCGGCAGACCTTTCCTTCACCTCATCA23.4482758620689653No Hit
TTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTT23.4482758620689653No Hit
GCCACAAGCTGTCCAGTCTAATCGACAGGATTCCGATTCCTGAACAGTGT23.4482758620689653No Hit
ACAAAGGTCATGGTTTTTGTTACTGACTTAACTCCCTTAAAAATCTTCTT23.4482758620689653No Hit
CGGCTACTGCGTGCCCGCGGACGCGAGGAAACGATGATCCTCGGCTCCGA23.4482758620689653No Hit
AGTTCATTAGATGATTCCATTCGATTCCATTTGATGATGATCCCATTCGA23.4482758620689653No Hit
GTGTAGATTATACTACAGTGGTAAAGAAAGTAAGCAGCTGGCAGTGAAAG11.7241379310344827No Hit
GTCACTGGGGAGAGAGCAAAGCCAATTCCCTGGCCTCTCAGATCCACACA11.7241379310344827No Hit
GGTAAAAGCAGTGATTTGTTCAGCTTTTGCTAGGTTATTCTGTTATAACA11.7241379310344827No Hit
CTCTTAAATAGTATTGCTATTGTATAAATACTATAGGATTTCACTTCAGA11.7241379310344827No Hit
CTATATGTTTCTGTATTTCTATAAATATTCTTGAGCTTTGTTCTGAGATT11.7241379310344827No Hit
GTTAAGAGCTACCGATGGGAAGAAGAAGATCAGCACTGTGGTGAGCTCCA11.7241379310344827No Hit
TGTGAGCCCTGTTTATAATGCACTTGTTTCTGTCTCATGCAGACTGGATG11.7241379310344827No Hit
AAATAAATCAGAGTATGTTTATTATGAATGAAGGATAGGGCTCAATATTG11.7241379310344827No Hit
GGAAATTAATTTTATTTTTAAATCCAAGGAGCTTTAACCGAACAGGAGAG11.7241379310344827No Hit
ATCCATGAAGGGGCTAAGGCCTGAGGAGGAACTTGCCGGGGTGTCTCATC11.7241379310344827No Hit
CTGCATTCTGATCCACTCCACTCAACTCCACTCCACTGTTCTCCATTCTG11.7241379310344827No Hit
GTAAGGCATGAAATGAAACAAAAGTTTTGAGCTGTAAGGTGCCTATAAAG11.7241379310344827No Hit
GGCTAGAGGGGTTCTGAAATTTTTGTATTTGTAGTAGAGATGGGGTTTCA11.7241379310344827No Hit
AAACGGAATTATGGAATAGAATCGAAGAGAATCTTCGAACGGACCCGAAT11.7241379310344827No Hit
CTCGCAGTACTACGACAACTTGCACATGCTCACGCTGGCCCGAGGAGGCC11.7241379310344827No Hit
TCCTCATATACTAGAACAAAGTCAATTCTTCGCTGGCCATCATTAAAAAA11.7241379310344827No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA11.7241379310344827No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG11.7241379310344827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGCTTAC11.7241379310344827No Hit
AGTGCAGAGCGGCCGGCGTAAAGCCAACCCCCAAGACAAAGTCCACCCCA11.7241379310344827No Hit
GTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGCC11.7241379310344827No Hit
ACTATGAAGCAAAGTATTCTCCAATCGTGGCTGCATTAGTGTCCATCCGC11.7241379310344827No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC11.7241379310344827No Hit
CTACCCATGCTCTACCTCAATCTGATCTGTTCACAGTCCGACACTGGTTA11.7241379310344827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGCCACGCTGGCAGAAACTGAGTTGCTGGTGTGGCAAAAAGATAGTGA11.7241379310344827No Hit
GCAATTGACGCTGTTGCTGTCATTGGTGGCGTTTGTGGGCGGCGGCCTGA11.7241379310344827No Hit
GTCTTCAAATTCATCAGCATTGAACTTGGAGACAGGCCTTTCAGGTGGAT11.7241379310344827No Hit
GATTTCTACCATCTTAGGTGTCAGCTCCTGGCTAAGGGGCTAGGCGTTTA11.7241379310344827No Hit
GATATGCAGCTTTCTTTTAGTAATGGACTCATTTATAATTTGAAATACCA11.7241379310344827No Hit
CTTCAAGGGTGCCTATTACCTGAAGCTGGAGAACCAAGGTCTGAAGAGCG11.7241379310344827No Hit
CAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAGGAAGACCTC11.7241379310344827No Hit
ACTGTGGGCTGCAAGCCTTCAATAGTCATTTCTGTTTGATCTGGACCTGC11.7241379310344827No Hit
CAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTC11.7241379310344827No Hit
GTCTTAAACTCGTCCTGACTTTTCCCGCTGGAGCGAATGGCCGAGACCAA11.7241379310344827No Hit
TCTACCTGCCGGCCGTGAGCCTGGAAATGCTCGGCGACCACGCCAAGATG11.7241379310344827No Hit
CTAAACAGCTTGTGTAAAGCAAAGGAAACAATCAACAAAGTGAAGAGTCA11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)