FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTGTCCATCACTATCCCATGTTCCTATGATAGGTGACTTTGGCATC34.615384615384616No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23.076923076923077No Hit
AAATTAGACAGAGAAGCTGATGAGAAAACTTCTGGTCCAGAATACATCAT23.076923076923077No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC23.076923076923077No Hit
GCACCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCT23.076923076923077No Hit
GGAGATAAGTGATGACCGGCCAGATTATCGCTGGACGAGACTTGGCGGCG23.076923076923077No Hit
TCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA23.076923076923077No Hit
AAATCCACCAAGCCAGTGGTTCCACTTGCCATACAAAGTGTCACCACTGG23.076923076923077No Hit
GGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCAATCTC23.076923076923077No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAAC23.076923076923077No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT23.076923076923077No Hit
GCCCTGATTATATTATACTGGAGTTCCCTCCAGTCTCCTGCCTTCTTGCT11.5384615384615385No Hit
CATTTGTAGTTAAGGTTATCACATCCCTGCCAATTTTACTAGATTTTTTC11.5384615384615385No Hit
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC11.5384615384615385No Hit
AGCCTGGATGTAGGCCTTCGCGTCCTGCCACGCAACCGACTTGCCGGTGC11.5384615384615385No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT11.5384615384615385No Hit
TTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC11.5384615384615385No Hit
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT11.5384615384615385No Hit
GGGATAGAATGGAATCGAATAGAATAGAAAGGAATTGAATAAACCCGAGT11.5384615384615385No Hit
CTCCAGCTCTACCAGTGTCCTCCTCACCCTTGACAACAACGTGGTGAATG11.5384615384615385No Hit
CCTTTTACTGATGAGGAAACCAAGTCAAGAAGTCATAAATGACACACCCA11.5384615384615385No Hit
CTGCAAACATGACCAGCCCGTCATGACGATGAGGTGTGGGGCGTCGCAAC11.5384615384615385No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCACTCATAATCCCACAGAT11.5384615384615385No Hit
TTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCC11.5384615384615385No Hit
GCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGAC11.5384615384615385No Hit
GATATGCAGGTCTTCTCTGGGCCACAGAGCCTCACAAAACCCACTGTGAG11.5384615384615385No Hit
ATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGC11.5384615384615385No Hit
TCCATGCACCGGGTGGGGAGTCCCAACGCCGCTGTCCTCTGGCTCTGGTC11.5384615384615385No Hit
CCATTGGACTATGGCTCCGATTCGACTCTCAGACCAAGAGCATCTTCGAG11.5384615384615385No Hit
GCTCAAGAGATTCTGGCTCGTCACCTCAGCGCGTATCCGGAGACAGGGGT11.5384615384615385No Hit
AGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTG11.5384615384615385No Hit
GATGATGGGCGGCTTCAAGGAGACCAAGGAAGCTGAGGACGGCTTTCGGA11.5384615384615385No Hit
GAAATAATCTTGATCATCACGATGATGGGCTCATTTTTCAAACTACCTCT11.5384615384615385No Hit
CTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCC11.5384615384615385No Hit
ATCCAAATGAGAAGGAATCAGAAAGATAATTTTAGCAACATGACAAAACA11.5384615384615385No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG11.5384615384615385No Hit
CCCTCCATGTGGCTTCAAGCCACCAGGACACAGCCCCCCCCAACACTCTT11.5384615384615385No Hit
GCCATCGCCTCGTGCGCCACGCGACGTCGCGAACAGCCCTCGTCATGTCT11.5384615384615385No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT11.5384615384615385No Hit
CCGCGAGGCTGGCCGCGACCGCCGCGCCCGAGCCCGACGACGAGCCGCAG11.5384615384615385No Hit
AGGAGAACCAGAAACATATCTATTATATCACAGGTGAGACCAAGGACCAG11.5384615384615385No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11.5384615384615385No Hit
CTTCCTATGTTGTTGAAGTATTCGGAGTATTTTAACTACGTCTCTCAACC11.5384615384615385No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT11.5384615384615385No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAAT11.5384615384615385No Hit
GTTCAAGATCAGCCTGGCCAACATGGTGAAACCCCCTCTCTACAAAAATT11.5384615384615385No Hit
GTCTTGACTTGCCTGGTGGTGGCCTTCCTGTTCGTCGGCAGCCTGACGGA11.5384615384615385No Hit
GATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGG11.5384615384615385No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA11.5384615384615385No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG11.5384615384615385No Hit
TGTCACGGCGCAGGGCAAAATATCCCAGCGAATCAGCCGGATTTTCGTCA11.5384615384615385No Hit
ACTCTGAACAATGTAGACATCCTCTCCACGTACACTTTCACCAATTTCCA11.5384615384615385No Hit
CTATCTTCCTTTTTTTCTTCCCAAACATACACCCACGCATACCCACACAA11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)