FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTAATCCCAGCACTTTGGGATGCTGAGGCGGGAGGATTGCTTGAGCT34.615384615384616No Hit
GGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAA23.076923076923077No Hit
TGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGT23.076923076923077No Hit
GATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTAT23.076923076923077No Hit
GTTCGAGGACCCGCTCCTTGAGCCGCGCGACGGTTGCATCGTCAAGATAA23.076923076923077No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC23.076923076923077No Hit
CAGGAAGATAGTTACCAGCTTCATGACAGTTATCTGGGATATTTTTCAGG23.076923076923077No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG23.076923076923077No Hit
ATTTTATCCTCAAGTTTTGCAAAAAAAATTTTTCCGCCTTTTTCTAAAAT23.076923076923077No Hit
TGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA23.076923076923077No Hit
CCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCA23.076923076923077No Hit
GAGCTGGGTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAT11.5384615384615385No Hit
GACTGGTACCGGATCGTGGGTGACACTTCGATTCCCATGACTCTGGCCGT11.5384615384615385No Hit
ACTCGCATCCTTCAAGGTAGTGGAGCAGGTATCGCCCAGGAGCCGACGTC11.5384615384615385No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11.5384615384615385No Hit
CCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAG11.5384615384615385No Hit
TTTTATGTATGTGCATTTTTTAATCTTTATGTTGTATTTCCTTGAGTAGC11.5384615384615385No Hit
ATATCGGATGAATCTGTACGCCCGGTGCTGTATACATCAGCGAGTCCATG11.5384615384615385No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG11.5384615384615385No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT11.5384615384615385No Hit
GTATCAACGCAGAGTACATGGGGAAGATGGCGGAGTAGCAACGCAAAGCG11.5384615384615385No Hit
GCCAATCGTCTCGGTGTGCCTTTCGCTGTGGTGCGGATTCTGTCCGACTC11.5384615384615385No Hit
GAATATAGACTCCTGTGTAGAAGCTGGAATTATTATTATTAGTTTCTTGC11.5384615384615385No Hit
ATTTTCAATTCAATTAAACATAGTAGGCACTCAACTCATATGTTTAATTG11.5384615384615385No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTG11.5384615384615385No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT11.5384615384615385No Hit
GTCCTATTTGTACTTTGCTCCCGTGTTGAGAAACGTAGGTTTTTTTGTTT11.5384615384615385No Hit
ATTCCATTGCATTCCATTGCATTCAACTCGGCTTGATTCTATTCCATTTC11.5384615384615385No Hit
GAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCGTCCGGTG11.5384615384615385No Hit
ATCCAGGCGTTCATTGTGGCAGGCAGAACAAAGTTCACGGGGTGGCCACT11.5384615384615385No Hit
ATGCTGGACAGCTCATCACAGGAGATGCCGCAGATGGCTTGCAAGTCCTT11.5384615384615385No Hit
AAATTACGGAAACCCTTGTGGGAGGGTAAAAGAGGTGACTCTAGTGGGAG11.5384615384615385No Hit
ACACTAAAGTCTTCCCCTCAAATTCCTTCAGCTGTTGGACACAGTACTCA11.5384615384615385No Hit
GCCCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGA11.5384615384615385No Hit
GCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCA11.5384615384615385No Hit
TCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGG11.5384615384615385No Hit
GGTTTGAGGGTACGGTGGGCCAGACGCACCCCTGTCTCCGGATACGCGCT11.5384615384615385No Hit
GAAGGCCCGCATTGCAGCAGCAGCAGCGAGATCGGGCAGATCTTCGCACG11.5384615384615385No Hit
CAGACCACCAGCATTACCGCGGCCGCCGGGGTGCTTCCGGTGCTTGCCTA11.5384615384615385No Hit
CCACTTCCCAGCCTTGGGTGTGTCATTTATGACTTCTTGACTTGGTTTCC11.5384615384615385No Hit
GATCCTTAATGCATTTATTTTAAACCACAAAATCAAAATACAGCAATCTT11.5384615384615385No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG11.5384615384615385No Hit
GTTCAGGGTTGAGGGATGGGTCTGCCTTGCTGTTGGCTTCTCGTGAGATA11.5384615384615385No Hit
GCTCGGCGTCGGGCACGCGCGCGGCCCACGCCGCCCGCCACGCCGCCCGC11.5384615384615385No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC11.5384615384615385No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC11.5384615384615385No Hit
GTCTCAGGCAACTCAAGTTTAGACCCTTACTCTTTTTGTTTGTTTTTTGA11.5384615384615385No Hit
GTTTAGACCGTCGTGAGACAGGATAGTTTTACCCTACTGATGATGTGTTG11.5384615384615385No Hit
ATCCGAACCTGACGGTGCCGATGGGGCTGGTCGACGGGTTGCCGGTGGGC11.5384615384615385No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG11.5384615384615385No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA11.5384615384615385No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT11.5384615384615385No Hit
GGCTGAGGCAGAGGATGTAGTGAAGGGTTGACTGATTTCTGAGAGAGGTA11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)