FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACCCGTCGCCCGCGTCCTGCAGCTCGACATCGGCGGATGCGGCGAC24.761904761904762No Hit
CCTCCGCGCTGACCGCCTCGCTGTTCAGCTGGTTCTTCGTGTTCAACGTC24.761904761904762No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC24.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGACGTTGGTGCCGGTCGCGCTGCTGGTCGCCCGCGAGGCCGCGG24.761904761904762No Hit
GCCTCAGCCTCCCAAGTAGCTGGGATTACAGTAGACAGCCACCACGCCTG24.761904761904762No Hit
GTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCAC12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCCCACGTCCCGTCACGCCTCGGCGTGCCCCCGCCTGGCGGCGGAGGC12.380952380952381No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATACACATCT12.380952380952381Clontech Universal Primer Mix Long (96% over 25bp)
GACCCTGGCCCCGGGTTCCCGTGGCTACCGGGCGCGGCCGCCCCCGCGCC12.380952380952381No Hit
CTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAAAC12.380952380952381No Hit
GCACTCCAGCCTGGGTGACAGAATAAGACTCCATCTCAAAAAATAAAGAA12.380952380952381No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGACGAG12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GATCTGGACGAGTTCACGGAGACGCGGTACCAGCAGTCGGCTATCCGGAA12.380952380952381No Hit
TCTTCCGCATGAATTGGTACCGGATAAATATTATGAACAGTACAAGGATA12.380952380952381No Hit
TATCTGGACCCAGAAGGGACTTCCCTGCTCGGCTGGCTCTCGGTTTCTCT12.380952380952381No Hit
TAAAAAAAACAAAGATTTTATCTATTAGCATTTCAATTTTTGCATTTATT12.380952380952381No Hit
TCCCTGACGCATATTTTCACAATTATTCTAAGTGTTGGTTTATCGTAAGA12.380952380952381No Hit
ATGCAGCGTAAATCGTCGCGGCCTATGGCCAAGACCGAGCGCGTGCGCAC12.380952380952381No Hit
TGATGTCACTGTGGATGATTTCGGCGGCGTGCGCCGCCTTGAGCGCAGAC12.380952380952381No Hit
ATATGGTAGACTGTCACAGAGCCGAATACCAGTAACAGGATGAATCCAAT12.380952380952381No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GCACCAGGAAGTCAGAGGTGTTGACAGTCACTGGCCCATCCTGGTCGATC12.380952380952381No Hit
CTACAAAGGGAAGTACGTGGTCCTCTTTTTCTACCCTCTGGACTTCACTT12.380952380952381No Hit
TGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGAAAC12.380952380952381No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGAAACACTGGGAAGCCATCAAAAAAGCCGCTGAAAGCCGCCCGGCC12.380952380952381No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTAACCTCTTTTTCTCACCGCTGGTACCGTTGACCGATGCACTGGCG12.380952380952381No Hit
CACCACAACACTTCCCAGGCATGAGCCCTCAGGGGCCCACATGAGCTTCC12.380952380952381No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACGATGCGTTGAT12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
AGGCCAGACTGTCCGCCGGCAAGGCCCGCGCCGAACAGCTCAGCCTGCGC12.380952380952381No Hit
ATAGAAGGTCATATAAGCCCGAATTCGACATAACATTAGGCCATGATGCA12.380952380952381No Hit
ATTTGTAAGAATAAGTAAGTCGCTTCTAGTCATCAGGGTGTATTATCCTG12.380952380952381No Hit
AACCAGGACCAGATGAGGTAATCAATCATAGAGATAGAATTGCCACCAAA12.380952380952381No Hit
GATCAGGGCCTCGGGCCGGATCAGGTTGTGTGGGTCGCCGTCGCTGGCGA12.380952380952381No Hit
CTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCT12.380952380952381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)