FastQCFastQC Report
Fri 10 May 2024
EGAF00003659780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Total Bases5.8 kbp
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGATCCAGAAGAACACCGTGATCTGCACCTCGACTCCGGCAATCG24.761904761904762No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGACCTGCTCGCGCGTTATCCGGTCGCGCTGCTCGTACGCGACGCAT24.761904761904762No Hit
GGCCAGGCAAGGGGGCTCATGCCTGTAATCCCAGTGCTTTGGGAGACCAA24.761904761904762No Hit
GCTGGGACCTCGGCCCGGTCTACCGGCTCGACGGCGGCACGGCGCTGGGC24.761904761904762No Hit
ACTCTGCGTTGATACCACTGCTTCCCTTCTCTGCGTTGATACCACTGCTT24.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATCGGGCAGGACGGCGGTGTCCATTACATCGCCACCTAACTCGTCGAA12.380952380952381No Hit
GGTCTGGCCTTTCCTGGGTCAGCATAGGGCACCCAGGTGGGGGCACAGGT12.380952380952381No Hit
GCCAACGGTCGCACCGAGCGTGGGGGGGGTGGGGGGGGGGGCGGGGGGGG12.380952380952381No Hit
ACGCAGAGTACATGGGGAGCTCGGCCTGCGCTGCGCCACGATGTCCGGGG12.380952380952381No Hit
GTGTATTTGTCAATTTTCTTCTCCACGTTCTTCTCGGCCTGTTTCCGTAG12.380952380952381No Hit
GACCTGCGCGCCGAGGCCGACCGCATGGCCAATGGCGAGCACGAGGAATA12.380952380952381No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATAGGTA12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GTACGCCTCTGCTCAGCGAGCTGATCCTGCAAGAGCCGCACAACCTCGGC12.380952380952381No Hit
GATAATACACCCTGATGACTAGAAGCGACTTACTTATTCTTACAAATCTG12.380952380952381No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGGTA12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG12.380952380952381Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACACATCT12.380952380952381Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACT12.380952380952381No Hit
TTTCCATAATGGCTCCACCATTTTTGATTCCACCAGCCATGTACAGGGGC12.380952380952381No Hit
GGTGTCCTGGTCTCCTCGGCCGACGCTGGCGGTGGAACCGTCCGTGGCGC12.380952380952381No Hit
CATGGGCGCTATGTGCCCCCTAGCAGTACCGATCGTAGCCCCTATGAGAA12.380952380952381No Hit
ATCTGAGACAGGTAGCCATGTATGATTCCGATCACAAATAGTATGGGTGG12.380952380952381No Hit
TATTCGTAGAAGCAGACACAGAAGAGAGGCCGTGGCCGCTCTAGTAGACT12.380952380952381No Hit
CCTCATACCATACATTTTTGCCCATGTGTCCATGTGAACCTAACATTTCT12.380952380952381No Hit
CGCTTCTCGGTCAGGCGCTCGGATTGCAGCTCCAGGCGCTCCAGGGCCTG12.380952380952381No Hit
TATCAACGCAGAGTACATGGGGAAAAAGGAGGTCTCACTGAGCACCGTCC12.380952380952381No Hit
GGCATTTAGTGCTACAAATTTCCCTCTACACACTGCTTTGAATGTGTCCC12.380952380952381No Hit
CCCCCAGATTGGCCTGGTATCTGGATGGACAGCTGCAGGAGGCCAGCACC12.380952380952381No Hit
ACCTGGTAGCCCAGGAGCTCCTGGGCCTCAGCCTGCAGTCGGGCCCTCCT12.380952380952381No Hit
ACGTTGAGCTCACTGTTGGACCCGAAGCTGCAAGAAGTGCATAAGACGAT12.380952380952381No Hit
GTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGGAAC12.380952380952381No Hit
CCATCACCCTGCCCGCCGACTACGTCTCCCAGATGGTCCAGCTCGGCTAC12.380952380952381No Hit
GAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGAT12.380952380952381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)