FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT23.6363636363636362No Hit
GGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAAT23.6363636363636362No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT23.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
AGATGAAACAGAATTCTATTTGGGCAAGAGATGCGCTTATGTATATAAAG23.6363636363636362No Hit
AACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCA23.6363636363636362No Hit
GCTTGGCGATGGGCAAGCCGTGGCGGCAGACCGAAGGACTGCTGCGCTCG23.6363636363636362No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
ACCTTAGGAGGCCGAGGCAGGAGGATCCTTGAGGTCAGGAGTTCGAGACC23.6363636363636362No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAATTA11.8181818181818181No Hit
GTGTGTGGGTCGCTGATTTCTTGGAGCCTGAAAAGAAGGTAACTGGGCAT11.8181818181818181No Hit
CCCTCCACGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGG11.8181818181818181No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATAC11.8181818181818181No Hit
AGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCC11.8181818181818181No Hit
GTTACAGCTTATACTCCCCAGTTGAGGACCTGTGTCATTCTTAGTGGCCC11.8181818181818181No Hit
GCGCGGACGAGCTGCGGGCGCTGCTGCGGGTGCGCTGGGCCGCGGGCTGC11.8181818181818181No Hit
ACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAG11.8181818181818181No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA11.8181818181818181No Hit
CCTGTTAATCTGTCTTTGGAAAGATTAAGCTGGAAAACCTGAAATGAGTA11.8181818181818181No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11.8181818181818181No Hit
CATTTCCTTACAGACATCACCTTGTCAATTAGAGAACACATAACGTTGAT11.8181818181818181No Hit
CCTTTATGTGTTCAGTAGTCTTAAGTTTTTAAGGTAGTGTTGAAAAAAGT11.8181818181818181No Hit
GCCCGCGGTATTGACGTTGATGACGTGACGCATGTTATCAACTACCAATG11.8181818181818181No Hit
GCTTGGAACCAGCAAATATTCTTCTCCTATTGGCAAAGCAATCTGGAATT11.8181818181818181No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11.8181818181818181No Hit
ACACAGATGGGTGCCATGGCAGATGGCGCAGATCAAGTGTACCTTCGTCC11.8181818181818181No Hit
GTCGGTTAGAGCGCATCGCTGATAACGATGAGGTCGCAAGTTCGATTCTT11.8181818181818181No Hit
GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA11.8181818181818181No Hit
CAGTTATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG11.8181818181818181No Hit
TCTCAGTAGGAGCCTTGAGATTTCCAAGACTATGAAGATATTCTACATTT11.8181818181818181No Hit
GGCTTCCGGCGCCCCAATGCTCATCTCCAGAACCATCTCCGTGTGTTTCT11.8181818181818181No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.8181818181818181Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCT11.8181818181818181No Hit
GTGTGTGGGTCGCTGATTTCTTGGAGCCTGAAAAGAATGTAACTGGGCAT11.8181818181818181No Hit
ATATGCCCATGTCCTAATCCCTGGAACCTGTGAATATTACCTTATATGAC11.8181818181818181No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA11.8181818181818181No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT11.8181818181818181No Hit
GCTATATGAGTATTCTGATAACCAAGAATACACTACACCAACTCAAACTG11.8181818181818181No Hit
TTTAAAAAGTCTGCCCTACTGATCTTCTCTCTTTTCTTTGCAAAATGGTT11.8181818181818181No Hit
CAGCCAGCCCCCAATCTTCTCCACACAGCCCTCCGTATGGATCGCCTTCT11.8181818181818181No Hit
CTCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGAGATCATGCCACTG11.8181818181818181No Hit
CCTCAGGAGTCGTAAACTGATGTTTCAGGGATGGGCCCATGACTCACTGG11.8181818181818181No Hit
CCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATC11.8181818181818181No Hit
GCTCAGGGGTGGGGTGGCTGGGAGCATGAACTTCAGGTCCAGGAAGATGG11.8181818181818181No Hit
AGTCCTTTGCTTATTTTGCACATTTTTCGTTTTCATTCTTTTACCACCTT11.8181818181818181No Hit
TAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCT11.8181818181818181No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)