FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.6363636363636362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTGCAGTGATTCTCCTGCGTTCAAGTAATTCTCCTGCCTCGGCCTTC23.6363636363636362No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG23.6363636363636362No Hit
GTTTAAATCCTTGAGGGGTACAGCATCACTCGGATTCTGTGTCCAATGGC23.6363636363636362No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23.6363636363636362No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC23.6363636363636362No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.6363636363636362Clontech Universal Primer Mix Long (96% over 26bp)
CGCCAGCTGCGGCGCGGCTTGCCGCCGTGCTTCTCATGCAGCCACTCGCC23.6363636363636362No Hit
CTCCAGGGACACCAGGGGCTCCAGGCAGCCCATTATTCCCAGGAGGACAT23.6363636363636362No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT11.8181818181818181No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC11.8181818181818181No Hit
CCTCTGAGCAGTGTATGTCAGGACTTGTTCATTAGGTTGGCAGCAGAGGG11.8181818181818181No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT11.8181818181818181No Hit
GTCTTATATGGATACATATGATTACATTTAGAGTTTCCCTGAATTCTCCA11.8181818181818181No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11.8181818181818181No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.8181818181818181No Hit
TACCCGAGGGTACCGTGGGCCTAGCAAGAATCGAACTTGCGACCTCATCG11.8181818181818181No Hit
NTTAGTGTTTTTCCCCAAAAGTATTGTGCCATTTTAGTTAATTATAAAAT11.8181818181818181No Hit
ATAAAAGCTGTGGTTCAGATAAATGATACAATAATCTCGGCTACTACAAG11.8181818181818181No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA11.8181818181818181No Hit
TGTAAAATCATGGCACAGATTTTGACAGTGAAAGTGGAAGGTTGTCCAAA11.8181818181818181No Hit
CCCACTTCACCTGTTACGTCAGGGTCAATATCGAGATCCGTATACAGATG11.8181818181818181No Hit
ATCTTTACACAATGTCACGGGACCAGTATTGTTTACCCAATTTTTTATAA11.8181818181818181No Hit
GCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAAC11.8181818181818181No Hit
CACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATG11.8181818181818181No Hit
CAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCC11.8181818181818181No Hit
GAATGTAAGTATATTTCTTGATTATGGATCTGCCATGGCTGAATTTCGGA11.8181818181818181No Hit
GCACTGCTTTTGCTGCATCTCGTAAGTTTTGGCATGTTGTATTTTTGTTT11.8181818181818181No Hit
GTACATGGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGC11.8181818181818181No Hit
ATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTG11.8181818181818181No Hit
ACTAAAAATACAAAAATTAGCTGGGCGTAGTGGCGTGCGCCAGTAATCCC11.8181818181818181No Hit
ATCTTGAAAGGAGAAATTACACAAAACTCAGGGTGCTCTAGGGAAGTTTA11.8181818181818181No Hit
CTCCTGGGCAGATGATGGTTCTCCTGTTCTAGACCTCGTATCGTCTACAG11.8181818181818181No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.8181818181818181No Hit
TTCCACTTGTTGTACCATTCCAGTTCTGTACCCGGGCGGCAAAAGAATTG11.8181818181818181No Hit
CCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG11.8181818181818181No Hit
TTCCTAGAGTGAGTGATAGAGTGATGGAGTCTCACTCTGTCGCCCAGGCT11.8181818181818181No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA11.8181818181818181No Hit
GCTGAGTATTATATGTTTTACAAAGAAACACTGGGTCTTCTGGCTGGAAG11.8181818181818181No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA11.8181818181818181No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAAGGCGTTGTTACC11.8181818181818181No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.8181818181818181Clontech Universal Primer Mix Long (96% over 26bp)
TCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTA11.8181818181818181No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTGGGGAGTT11.8181818181818181No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGGGTTTATAC11.8181818181818181No Hit
GAGTTTAATAACAACTTCCGGCAGCAGATGGAGAATTACCCGAAAAACAA11.8181818181818181No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)