FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC25.714285714285714No Hit
GCTGCTGCCCGGGCATTCCGGGCATCCCCGGAGGCGCAGCGGGCGACCCG25.714285714285714No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC25.714285714285714Clontech Universal Primer Mix Long (96% over 26bp)
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG25.714285714285714No Hit
TTGTTATGGCTACTAATCAATTTGATGCAGGAAATCAATCACAACCACTT12.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATTTCGGCCCCAAAGCTCGTCTACTGAATGAAGGCGATCACGTTCAC12.857142857142857No Hit
GGCTGGTACCGATTTTTCTTTTCCATATTTAGTGCTTCCTTCAGAAGCCC12.857142857142857No Hit
TTGCACGGCATCGTACCGGCCGCTCCAGCGAAACCGCCGCAACGCTCACC12.857142857142857No Hit
CGGTAGGTTTCCACCTCCAGCTTCAAACGGTCGGATTCGGTTTTGCTGAC12.857142857142857No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
CTTTGATGTTGCCGCGGGGATACCTGAGAGCGACAGGTCCTTCGTGCTCG12.857142857142857No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.857142857142857No Hit
GTTCTCCCGTAGAGGGTGATCTTGAAGTACTCCCTGTTTTGAGAAACTTT12.857142857142857No Hit
CTGAAACTCCCCAGCACAGGGGAAGGGACCCAGTTCTACCTGTTTGAGAA12.857142857142857No Hit
CCTGAAGGGCTGCCCTGGCCAGGGAGCCTCGAATCTTCAGTCTCTCAGAG12.857142857142857No Hit
CTTCTGGAACTTGGGGTGGCTGCCCTGCTCCGTGCACAGCTTCTCCACGA12.857142857142857No Hit
CATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCA12.857142857142857No Hit
GTAATGGCGTTCTTGGCGTCGGGACCCTACCTGACCCATCAGCAAAAGGT12.857142857142857No Hit
GAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTGG12.857142857142857No Hit
AAATATGATAGATATGGCGATTCTGCTTTTGAGAATGGTGGAGCAGGATA12.857142857142857No Hit
GAGAGAGGGTAGGTTGCAGCGACTGCAGACCCACGGCAGAGAGTAAATGC12.857142857142857No Hit
GGGTTGATGTCGTTGCCGTCCGGCGGATACGCGAACTCGTGGCGCGCCTG12.857142857142857No Hit
GACGAATACGCGAGCCTGGTCGCCGAGATCGTCCGCAACGGCTACCTCAA12.857142857142857No Hit
TCTGGAAAGTTGAAAGGAGATGACCTTCAGGCCATTAAGAAGGAGCTGAC12.857142857142857No Hit
GTAACCATGTGCGACCGAAAGGCCGTGATCAAAAATGCGGACATGTCGGA12.857142857142857No Hit
TTCCTACACCGCCCGGCGCTGCTACTTTCGCGCAGGTTCTTTCACACCGC12.857142857142857No Hit
CCGAGCCCGTCTCGCCGCACACGATGATGACCTGGTGCCGGTCCATGGCC12.857142857142857No Hit
GAAGTACGCGCTCATCTCCGCCGCCGTGGTCGACAAGTCGGGCCGCCTGA12.857142857142857No Hit
ACGTGATCCAACGAGACGTTAATGCCGATGATGTCGGTATTGCCTCGGGT12.857142857142857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)