FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA418.91304347826087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT265.6521739130434785Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG132.8260869565217392Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC122.608695652173913Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG122.608695652173913Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG102.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102.1739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC81.7391304347826086Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG71.5217391304347827Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG71.5217391304347827Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA51.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA51.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40.8695652173913043Clontech Universal Primer Mix Long (100% over 23bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA40.8695652173913043Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40.8695652173913043Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.8695652173913043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT30.6521739130434783Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC30.6521739130434783No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.6521739130434783Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.6521739130434783Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC30.6521739130434783Clontech Universal Primer Mix Long (96% over 25bp)
AATCAATATATCAGAGAGATACCTGCACTTTCATGTTTGTTGCAGCTCTG30.6521739130434783No Hit
GTATATGCAGGAGCCCTACAAGGAAGATAACTTTATTTCCATTTCAGAAA30.6521739130434783No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.6521739130434783Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.6521739130434783Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTACATGCCCGTGATTCCAGCTACCAGAAGGCTGAGGCACGAGAATT20.43478260869565216No Hit
GCATAGTGGTGGGTGCCCGTAGTCCCAGCTACTTGGGAGGCCGAGGCAGG20.43478260869565216No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.43478260869565216Clontech Universal Primer Mix Long (95% over 22bp)
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG20.43478260869565216No Hit
CCACAAGACAGATGAGGGTGTTGGGCTGACCCAGTGTCACGGGAGACTTG20.43478260869565216No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTGGTATCCTGTCTCTTATACACATCTCC20.43478260869565216No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCATTGGTATCAAATCAAAAAAAAAAAAAAAAAAAAAAA20.43478260869565216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAAGGATAGTCCTGAGCCTAAAAAAGGAAACTCTTCCTTATTCTTCT20.43478260869565216No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.43478260869565216Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
ACATGATCCAAAGCAATGATCTTTGTAGTAAAGAAAGCATCTCAGGAATC20.43478260869565216No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCAGCAGGCGCTTGATGTAGTGGAATGAGTGACAGATGCAGGCGAGG20.43478260869565216No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA20.43478260869565216No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.43478260869565216Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.43478260869565216Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.43478260869565216Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTATACA20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
CAATGGAATCATCATCGAATGGACTCAAATGGAAACATCGTCAAATGGAA20.43478260869565216No Hit
GAATGACAGAATGAGATGAAAATGATAACTTGAGGAAAGGTGGCAAGGAC20.43478260869565216No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.43478260869565216Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.43478260869565216Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAAGAAAAATATTGGAATGGAATAGTGTTGGGAAACAGACATTAACA20.43478260869565216No Hit
CACACTCCATTGCATTCAGCCCGCTCTCCCAGTCATCACAGTCTGGTTTC20.43478260869565216No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCACTTAA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAGGGCAGGTAGGGAGACTTCCTCGACAGGGCCACCTGGATGCTGGC10.21739130434782608No Hit
CCCCTTCCCTGCCAGCCAGATAGATAGACAGATGGGAAAGGCAGGCGCGG10.21739130434782608No Hit
GCTCTTTACAGTTAACTCTTTTGTTTTATGACTCTCTTACTGGATATTTT10.21739130434782608No Hit
CAGTGGTACCATAGAAAATATTCAAATTGAGGCTGGGCGCAGGGCTCACA10.21739130434782608No Hit
CCTTTGAAAACTGGCACAAGAGAGGGATGCCCTCTCTCACCACTCCTATT10.21739130434782608No Hit
GGCAGATTGCTTTTTCTTTTTGCTTTCGGTTCACAGATAAGGACATTGGG10.21739130434782608No Hit
ATACTACAGTGTTACCCAGCTCCTCTTCATCAAGAGGAAGCTTAGATAGT10.21739130434782608No Hit
ATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.21739130434782608Clontech Universal Primer Mix Long (95% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTTTTTTTCAGCTGGGAGAGCAGCTTTATTATCATGGCTGGGGA10.21739130434782608No Hit
CTGTAGTTTAAAACAAAGAATCTAGGGGAAGGAAAAGTAGACAAAGAAAT10.21739130434782608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCACAGCTTT10.21739130434782608No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.21739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
CTCAAAATAGTGCCTTCTTCATAGCAGGCATTTGCTGTTGACTGACTGAC10.21739130434782608No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCT10.21739130434782608Clontech Universal Primer Mix Long (96% over 25bp)
GGCCTGATCAACCGTCTGGTCGAGCAGGCCGACGTTGCCTCAACCGCCAT10.21739130434782608No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACAGCTTCA10.21739130434782608No Hit
CCTGTAGGGCTTGCTTTACTGTGGAAGTCACCGTGCTGAACGTCCCCATC10.21739130434782608No Hit
CTCTCAGTCTGTAGCTATTTCTGACCTAGCTCCATGCTTTTATTTTTTAA10.21739130434782608No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.21739130434782608No Hit
CCCATGTACTCTGCGTTGATACCACTGCATCCCATGTACTCAGCTTTGAT10.21739130434782608Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGTGCCCGCCACCAC10.21739130434782608No Hit
ACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGCTTCCCAAGTACTCT10.21739130434782608Clontech Universal Primer Mix Long (95% over 23bp)
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT10.21739130434782608No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACA10.21739130434782608Clontech Universal Primer Mix Long (95% over 21bp)
CCCAAGTAGCTGGGGCTACAGGCATGTGCCACCACACCCGGCTAATTTTT10.21739130434782608No Hit
GCTTAAAATTACGTAAATGACTCACATTTCTGGCTCACATTGTATTTCTA10.21739130434782608No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
AAATTCCTTGCAATTTCTGGAAGTTGTTCATTTATTTGTCCCTGTGGGTT10.21739130434782608No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCACTGTCTCTTATACACAT10.21739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
GAGCGGAGCACCGACGGCCGCAATGCCACGGGCGGCGTGAGCAGCAACGG10.21739130434782608No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGAC10.21739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACACTGCGA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGGATGTTGGGGTCCACCTCCAGGACAAGGGGGCTCAGCAGGCTCTG10.21739130434782608No Hit
TCCCTTTCGTTTACTCAAAGTCAGCTGATGAGGAACCTAATCATATTTAC10.21739130434782608No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG10.21739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
CCCTCGTGCTGTCAACTGCCGCAAGAAGAAGTGTGGTCACACCAACAACC10.21739130434782608No Hit
CTTTTCCCCTCCACCCAGGCCTCCCGAGCTGCCAGGCTCTGTGCTCCCAC10.21739130434782608No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACCACTGCTTCCCATGTAGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACTGTCTCTT10.21739130434782608Clontech Universal Primer Mix Long (96% over 27bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
AAAGGAAAGAGTGAAAGAGAAAGACAGGGAAAAGGAGAGAGAGAGGGAAA10.21739130434782608No Hit
GCCATGGGTTACTCCTTGCCCTTCTCAGGGGCAGTGGGCTCCCAGAGCCA10.21739130434782608No Hit
AAAAAACCCTGGCCCTTGTTTCTTCCAGTTTCTAGAGGTATCAGCTCCTA10.21739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
AAAATGTACAGCTCTTAAGTGTACAGTTCGGTGAATTTTGACAAATGTAA10.21739130434782608No Hit
GAATAAGGCCTGTTGTTCCTTGCAGTGGATCCTGATTTGGACAAGCAGCA10.21739130434782608No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGCGCCTGTAGTCCCAACACTTTGGGAGGCCGAGGTGGGCG10.21739130434782608No Hit
CCTTTCCTATTATCCAGGAGTAGCCATAGCCAGTGGGAAATATCAGCTAT10.21739130434782608No Hit
CTCCCAGAGAGCAGATGGTTGAAGATTATACTCGGCTACTTTGTTGCTGA10.21739130434782608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.21739130434782608No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATT10.21739130434782608Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10.21739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTAT10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG10.21739130434782608No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.21739130434782608Clontech SMART CDS Primer II A (100% over 26bp)
CCCAAGTACACTGCGTTGATACCACTGCATCCCCATGTACTCTCAGTTGA10.21739130434782608Clontech SMART CDS Primer II A (95% over 24bp)
CCATAGAGAATTGTAGCATATCTCAATGTCCTTGAAAGAATAGAGAGGAA10.21739130434782608No Hit
TTCCAGGTGGCCACGAAGAGTGCCACGTTCCGGTCTGGGAAGTAGCGGGC10.21739130434782608No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.21739130434782608Clontech Universal Primer Mix Long (96% over 28bp)
ATCTTGCTAGGTTCCCAGGCTGAACTTGAACTCCTAGTCTCAAGTGATCC10.21739130434782608No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG10.21739130434782608No Hit
GTATCATCGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCCTGGCAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTACCTGTC10.21739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACACA10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTAAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTC10.21739130434782608No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTGGCTCACACCTCTAATCCCAACATTTGGGAAGCCAAGGGAAGAGGATT10.21739130434782608No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACTTGTTATTTTTTTTT10.21739130434782608Clontech SMART CDS Primer II A (100% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GATCAGAAGACTGTGTCCTGGCATTCTCCCTTCTTAGTTCCCCCACCTCC10.21739130434782608No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTA10.21739130434782608No Hit
ACATGGGATGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGT10.21739130434782608No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGGGGTATAAACGCATAGG10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCACTATCTCTGCTCAC10.21739130434782608No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAACAGGACCATGAATTCTTCTGGAGAGAGCACAGCAGCCACTGCAG10.21739130434782608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG10.21739130434782608No Hit
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAAGCAGTGGTA10.21739130434782608Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACCACGCCTGGCTAATTTTCTATTTTTGGTAGAGATGGGGTGTCACCA10.21739130434782608No Hit
GTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TCTCAGTACCACGCGGCGGCAATCTGGTCGAGGCCGCCACCGAAGCTGGT10.21739130434782608No Hit
CATGTACTCTGCGTTGATACCACTGCTTACATGTACTCTGCGTTGATACC10.21739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA10.21739130434782608No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
ACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACCTGTCTC10.21739130434782608No Hit
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA10.21739130434782608Clontech Universal Primer Mix Long (96% over 28bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.21739130434782608No Hit
CTAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAGGTTAGTTTT10.21739130434782608No Hit
GGTATCAACGCAGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.21739130434782608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.21739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TGCATGCATGCTCCACACATGCTCTCCGCTCCAGAATTTTTCTGAAGAAG10.21739130434782608No Hit
GTACCTCAGAGGGAAACATATAAGAAAGCTTGTATCGTATTCTTCCTCTA10.21739130434782608No Hit
GTTTCACCATGTTGGCCATCCTGGTCTCGAACTCCTGACCCCAAGTGATT10.21739130434782608No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG10.21739130434782608Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.21739130434782608Clontech Universal Primer Mix Long (96% over 25bp)
CGCTACGCCTGGGGCACCATCTGGACCCGCCCCGGACTGGAGCGCACCAC10.21739130434782608No Hit
CTCCACCACGCCCGGCTAATTTTTTATATTTTTAGTAGAGACGGCGTTTC10.21739130434782608No Hit
GGCACTGCTTGTGGCCCTAGCTGTGTGGGAGGCTTAGGTGGGAGGATCCC10.21739130434782608No Hit
CAGGAATGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGT10.21739130434782608No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GGAGCTTGCAGTGAGCCGAGATGGCGCCACTGCACTCCAGCCTGGGCGAC10.21739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTCCGCTACGCGTTCAACCCCGACAACGGATCGGAGGCGGCCCATGG10.21739130434782608No Hit
TTTTCATCCCATGTACTCTGCGTTGATACCACTGCTCCCATTTAATCAGC10.21739130434782608Clontech SMART CDS Primer II A (96% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGCACTGCAACCTCCGCCTCCCGGTTTCAAGCT10.21739130434782608No Hit
CACTTGGATCAAATCCAGAAAATTGAAGTATTCTGCACCGAAACTGTCCA10.21739130434782608No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAAGGAGTGG10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCAGGGGCCGGATGCCCAGCCCCGAGGCGGCCCTACTCCTCCTCCTC10.21739130434782608No Hit
CGTTCGAAACATCGGCCTCGCGTGTTGCACTGCCGAAAACCGCGCTGGAT10.21739130434782608No Hit
GGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.21739130434782608Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACAATGC10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TAACGGTTGATAAATTTTAAAAAGTGACTTCCATGAGCCATTTATTCATC10.21739130434782608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAACAGCAT10.21739130434782608No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCAACCTCC10.21739130434782608No Hit
GTTTAAAATGACATTTGGAAGATTCCAGAAGGAAATGGAAAGACCTGGGA10.21739130434782608No Hit
ATCAACGCAGAGTACATGGGGATATCAGAGACTGAAGATCAACTTAATGA10.21739130434782608No Hit
ACTGTGCTCCAGCCTGGGTGACCTCTTGTCAAAAACAAAAACAAAAAAAA10.21739130434782608No Hit
GACTTGGGAGCAATAAGACTTTAGAAAAAACCTTACAGTGTACTAATTAT10.21739130434782608No Hit
TCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTA10.21739130434782608Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACTCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.21739130434782608Clontech Universal Primer Mix Long (100% over 23bp)
CCCTTAAAGTGTCAGGTTATTGAGAATCTGAGGATAAGGAAATTGGCATG10.21739130434782608No Hit
GTATCAACTCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.21739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.21739130434782608No Hit
CAACAGTATCTCATGAGAAAAAGTCCCTGTTTAAGACCCAAAGAATTTGA10.21739130434782608No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGTTAAAAAA10.21739130434782608No Hit
TGATACCACTGCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.21739130434782608No Hit
CTGCTTCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAGGGGGATGATGCCTCAGCTGTACCATTGGGAACCCAGACTACTTC10.21739130434782608No Hit
GAGGGCGATGAAGTTCTAGATCCATTGAGACAAGCTCTAGACAGTAGCAT10.21739130434782608No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAGAGTACTT10.21739130434782608Clontech Universal Primer Mix Long (95% over 21bp)
ATGATAGTATCCTTTTCCAATTCTCCCTGCTAAATTTCTGGCCAATCTGC10.21739130434782608No Hit
GAGTCCTGCTCTGTTTCCCAGGCTGGAGTGCAGTAGCGCGATCCCAGCTC10.21739130434782608No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATT10.21739130434782608No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.21739130434782608Clontech Universal Primer Mix Long (96% over 25bp)
CATGTATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC10.21739130434782608Clontech Universal Primer Mix Long (95% over 24bp)
TATCTTCACAGAGTGCCTTTATGACCAGTTTGGAGAATTACGATGGTAAG10.21739130434782608No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG10.21739130434782608No Hit
ATTTTAAGACATCTTGACCCTTTTAAAAAAGATCATTGGCAGTTAATCAA10.21739130434782608No Hit
CTCAGAGCTCCATGCAGTTTAAAACTTATGAATTGTTTATTTCTGAAATT10.21739130434782608No Hit
GGGTCCCTGCGGCTCTTAGGCGAAGGTGGAGTTGTTCCAGCCCACATTGG10.21739130434782608No Hit
AGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.21739130434782608Clontech Universal Primer Mix Long (95% over 21bp)
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC10.21739130434782608No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT10.21739130434782608Clontech Universal Primer Mix Long (95% over 21bp)
GATATCAATGATATCAGTGAGGAGGATTCTATGACTGGTCCAGAGAATGA10.21739130434782608No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAA10.21739130434782608Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)