FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA246.722689075630252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT205.602240896358544Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG143.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG133.6414565826330536Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC82.2408963585434174Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG82.2408963585434174Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG61.680672268907563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT61.680672268907563Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC61.680672268907563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51.400560224089636Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC51.400560224089636Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41.1204481792717087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41.1204481792717087Clontech SMART CDS Primer II A (96% over 26bp)
ACTAATAGGAGTTGCAGGGTCCGAAATAGAGTCTCACAGCCAGTTTAGGA41.1204481792717087No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA41.1204481792717087Clontech Universal Primer Mix Long (96% over 27bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.8403361344537815Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGTGACCAACATGGCGAAACCCTGTCTCTA30.8403361344537815No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.8403361344537815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTTCACTCACACCCCAGGTGGCCCGCAATGCCTGCTTCTCTTGTCAC20.5602240896358543No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GATTTCAGAGCATTGACCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAG20.5602240896358543No Hit
TGCCCCTCCTGTCTCGACTCGACCTCAGTCACTGCAGCCACCTTACAGAT20.5602240896358543No Hit
ATTCTGATAAAGCCAGACAAAGACATTTCAAAAAAAAAAAAAAAACTATA20.5602240896358543No Hit
GGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCACCTTGGC20.5602240896358543No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.5602240896358543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTACAGCTTCACCACCACGGCCGAGCGGGAAATCGTGCGTGACATTAAG20.5602240896358543No Hit
CCCATGGCACCTCCAACCAGGCTGCCCTTCCAAGGCACCCCATAAGAGAC20.5602240896358543No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA20.5602240896358543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAGAAGGTGTGGTGCCAGATTTTCTCCATGTCGTCCCAGTTGGTGACGA20.5602240896358543No Hit
CGCTTCGACGGCGCGCGACAACCGCATGCGCCGTGACGGTGGCGCGGGCA20.5602240896358543No Hit
ATGGAATGGAATGGAATGGAATGGAAAAAATTCGAGTGGAATGGAATGGA20.5602240896358543No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.5602240896358543No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGC20.5602240896358543No Hit
TTTTTATCATGAGGCATTTTGAGTCTATTCTTTGTGACACCAGAATTAGT20.5602240896358543No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTC20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC10.2801120448179272No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TAACAACGCAGAGTACATGGGAATCATATGTAACAACAATAAAAAAATTC10.2801120448179272No Hit
TGTACTCTGCGTTGATACCACTGCTTCCCATTGTACACTGCGTTGATACC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGGTATC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATG10.2801120448179272No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GCGCTGGGGCCCTGTGTAGAAGCTCCATCCCCCTTGTCTTTGTGCTTGCC10.2801120448179272No Hit
GCTTTCCATGTACTCTGCGTTGATACCACTGGGAAGCAGTGGTATCAACG10.2801120448179272Clontech SMART CDS Primer II A (95% over 23bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.2801120448179272Clontech Universal Primer Mix Long (95% over 22bp)
GAGGTGGGATGCCACAGATCGACGGTGACGGTGAGGTCGGATCCTCGATC10.2801120448179272No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CTGTCAATCACACAGGAGAAGCTGAGCAGAAAGCAAAGCAAAGAGGAATT10.2801120448179272No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
TCCCTAGAGTTGGAAGGACTCAAAAAGGCATCGCCACATACACTCCATGG10.2801120448179272No Hit
CCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAA10.2801120448179272No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATGTGTATCTTTAACCTTGAATTGTAATCCATAATTAGCCATAATTCAGG10.2801120448179272No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATCACCGTGCGCTGCCCCAGCTGCTGCAGGGGCCTGACATTGTCCT10.2801120448179272No Hit
AAATATAGCACTTTGACATTTCCAAAGCATTTATCTTACTTGACTCTCAA10.2801120448179272No Hit
GACAGTCTCGCTTTATTGCCCAGGCTGGAGTGCAGTGGCACCATCTCGGC10.2801120448179272No Hit
GCCTAAGTCTGTCATTTCTAATATGCTCTCCTAGTTTTAGCTCAGTAGTA10.2801120448179272No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
TGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAG10.2801120448179272No Hit
GATATAAAGTGAAGCAACCGCCTTAGTGCTGAAATATGTAGTACATGAAT10.2801120448179272No Hit
CAGCAATTCCAGGGTATACTATGTCTTTTTCTTTAGCAACATAACCTCCA10.2801120448179272No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT10.2801120448179272No Hit
AAAGAATACAAACCAAATCCAGGATGACCCAGTTTTAAGAGGTGACTTAC10.2801120448179272No Hit
TCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT10.2801120448179272No Hit
GGCTGTTGTGTGGACCAGTGGAGGCTCATTCAGTTCAATGCTGTGGAAAC10.2801120448179272No Hit
TGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CTAAAATTATGGATGAATTGAAGACACAGATTCACCGTAAAGCTGAAACA10.2801120448179272No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC10.2801120448179272No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACC10.2801120448179272No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTTTTAAC10.2801120448179272No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAAGTCTGCGAAGAA10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCTCTGCGTTGATACCACTGCTTCCCAT10.2801120448179272Clontech Universal Primer Mix Long (95% over 23bp)
GCATCCGCCCACTGCGTCTTCAGATGCTCGGTCGGGGCGACAACGACGAG10.2801120448179272No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTAT10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GGCCAGGGGAGGGGTGAGGGAAGCTGCAGGGTGCCACGATGAAGGACCTA10.2801120448179272No Hit
GTCATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTGAGGATTGCTTAATGCTGGCCACTGGTCTTCCAGTGGAATGTTCCA10.2801120448179272No Hit
CTATTCGACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAA10.2801120448179272No Hit
TGCCTGAACTGGGCATGGAGAAGGCATCATAATGTGCACCTGAACATTCA10.2801120448179272No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCACTGTCTCTTAT10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GTACAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGAAAAACAATACAAAAGCACCATTGGGTTGAGCATGGTGGCTCATGCC10.2801120448179272No Hit
GCTACTCAGGAGGCTGAGGCAGGAGACTCGCTTGAACCTGGGAGGCAAAG10.2801120448179272No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGT10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
GGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG10.2801120448179272No Hit
TCACAGCATACTCCCGGTCACAGTATTTGTCAAGGGTATCCTCCCAGGCC10.2801120448179272No Hit
TCCATGTACTCTGCGTTGATACCACAGCTTCCATGTACCTGTCTCTTATA10.2801120448179272Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCTG10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
CACTAAAAAATAAAAAAATCTCATTCCCAAAAAGAATTTGAGAGCAAAAT10.2801120448179272No Hit
GGAAATAGATACTTATTTTCAAGATGCTCAAACAGATGATTAGGCAAAAG10.2801120448179272No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC10.2801120448179272No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAGAGT10.2801120448179272Clontech Universal Primer Mix Long (95% over 23bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGCTGCAGTCCGTGGTCATCTAGGCCCTACTCCGCCAC10.2801120448179272No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCAT10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
AAACAACAATGTGCAATCAAAGTCCTCGGCCACATTGTGAACTTTGGGGG10.2801120448179272No Hit
GATTACAGGCGCGCGCCACCACACCCAGCTAATTTTTGTATCCCTAGCAG10.2801120448179272No Hit
ATATGGAGCCCAGAGAGGAGTGGATCCATTGCATCTGTTTAAAGATAGTC10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTGATA10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGAGCTGGCAGAGTTTAACTGGCTGAATAAAAAAGAGGTGGTTGCT10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTGGGCACTGTCGCTCCACTCAGCCTTAGAGTAGCTCCCTCCTTTTC10.2801120448179272No Hit
CTCTTATACACATCTCCGAGCCCACCTGTCTCTTATACACATCTCCGAGC10.2801120448179272No Hit
CATGTACTCTGCGTTGATACCACTGCTTACATGTACTCTGCGTTGATACC10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTC10.2801120448179272No Hit
ATTCCCTAGGCAGCACGCTGAGCAGCCCAGTATTGAGCAGTTTGCAAGGG10.2801120448179272No Hit
GTAGTGATTCCAGCGTCGGCGTATTTTGCTGGCTTAGCGTCTGACGCGCC10.2801120448179272No Hit
AGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTG10.2801120448179272No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCGAGGATATCCAGGAGGTCATCATCGCCACGAACCCCAACAACGAG10.2801120448179272No Hit
CTTCCCCACCACCTTGGCCCGGATCACGATGTCGGAGTTGCAGAAGGCGT10.2801120448179272No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGA10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGC10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAG10.2801120448179272Clontech Universal Primer Mix Long (95% over 22bp)
CTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.2801120448179272Clontech SMART CDS Primer II A (100% over 26bp)
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10.2801120448179272No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG10.2801120448179272No Hit
CCATGGAACACCAGGGATTGACAGCCACCACCAGAAGCAAGAAAGAGGCA10.2801120448179272No Hit
CAGTGGTATCAACGCAGAGTACAAGGGAAGCAGTGGTATCTGTCTCTTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GAAGTGGATGGGCCCTATACCTCCAGCCGTGAATGCCAGACTTTAAGATT10.2801120448179272No Hit
CCCTCTGGGAGCCCAACATGGATCCGGATCACCTGTTTGAAACCATCTCC10.2801120448179272No Hit
ACGCAGAGTACATGGGAAGCAGTGTTATCAACGCAGAGTACATGGAAAGC10.2801120448179272Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
AAACCTGAGTCCCTGGAGACGCTAAAGACTGAATGTCCCTGTAAGACAAA10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAAGTGAAGCAGTGGTA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
TTTTTATCATGAGGCATTTTGAGTCTATTCTTTGTTACACCAGAATTAGT10.2801120448179272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
TACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTGAGTCAAGCCAAAAAAAAAAAAAAACCAAAACAAAACAAAAAAAAC10.2801120448179272No Hit
GTATTTCCGGGGGCCCCTGCAAACCAAGGCCAAGTTCCACATTACAGTGG10.2801120448179272No Hit
ATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATG10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCACGATGCCGCCCGTGGACAGCTGGGCGTCCAACAGGCGCAGGCCCT10.2801120448179272No Hit
ACTGCAGCAGATGGGGCGGCGACGGCTTCTACGCCATGGACTACTGTCTC10.2801120448179272No Hit
CCCATTTCAGCGTCCTTCAGGGGAAACATTTTTGGAGTCCTAGGTCCTGT10.2801120448179272No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATTTTAAAGGATGGGACGGAAGCAAGTGCAGAATTCTGATGGTCTGTCCC10.2801120448179272No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAA10.2801120448179272No Hit
GTCTAGTGAAACTCCTTTCATCTCTGTCCTATGGCCCCACCCCACCATTC10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTTCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGATAAA10.2801120448179272No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
ATCGTAATGAGGCGTGCGCCGCCAATATGCACTGTACATTCCACAAGCAT10.2801120448179272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATAACAA10.2801120448179272No Hit
ATTTTTTATCATACGTTGCTGTCTCCTTAGCACAGCAATATCTGAACCGA10.2801120448179272No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG10.2801120448179272No Hit
CCACTGCTTGCCCATGTACTCTGCGTTGATACCACTGCTGCTACCCATGT10.2801120448179272Clontech SMART CDS Primer II A (96% over 25bp)
TGAAGGGGGGAGACCCCTTATATTTCCTGTTACTTGACTCACCAGTTTAT10.2801120448179272No Hit
CCGCACGGGTGTTCCTCAACCAGGCGCGCGAGGGGGCATCTGCCTCGGAC10.2801120448179272No Hit
GCCTAGAAGAACTGGCCAAGAAACACTGTGGGGAGGTGATGATCTTTGAA10.2801120448179272No Hit
GTTGAAGGAGATGCAGTATGTGAAATCCTTTGGAGTCCCAGCATCATCCA10.2801120448179272No Hit
GTACATGGGATTGAAAGTCAGCCCTCGACACAAGGGTTTGACCTGTCTCT10.2801120448179272No Hit
CAGCAAAGACAGGGGTCTTGAAAGCAGGTTGCCAGTAAGGCGTCTGTGTT10.2801120448179272No Hit
GAGCTTCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATTTGATTCTTCTCTCTTTTCTTCTTTATTAGTTTGGCTAGTGGTCTATC10.2801120448179272No Hit
TGTACTCTGCGTTGATACCACTGCTTCCCATTGTACTCTGCGTTGATACC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTTCGTAGATACCACACCTACCCA10.2801120448179272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.2801120448179272No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.2801120448179272No Hit
CACATATATATATTTTTGCATGAATTTGCATAATACATGTGCCACAGGTG10.2801120448179272No Hit
GATGGTGATCGCTCACACCAAATCCTTGGACCCCTCCCGGCCTGTGACCT10.2801120448179272No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGACTGTCTCATATA10.2801120448179272Clontech SMART CDS Primer II A (100% over 26bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAAC10.2801120448179272No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCTGAAGAT10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
TACCTGCTGGGGACGGAGCACGGTATCCAGGGTAAAGATCTGGGGCAACA10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)