FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA236.442577030812324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT236.442577030812324Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG123.361344537815126Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA82.2408963585434174Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC82.2408963585434174Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA71.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC71.9607843137254901Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG61.680672268907563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC61.680672268907563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG61.680672268907563Clontech Universal Primer Mix Long (96% over 26bp)
GAACATTCTGTGAACATTCCCTTTTTACTTTCTTCACTAAGGTTTGGAGA41.1204481792717087No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA41.1204481792717087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT30.8403361344537815Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT30.8403361344537815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.8403361344537815Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGTGCTATCTGGGAGATGCTTGGGTACAGGACAGTGTGTGCACTGA30.8403361344537815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.5602240896358543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTGGCGTACAGGTCTTTGCGGATGTCCACGTCACACTTCATGATGGA20.5602240896358543No Hit
TTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCTTTCAT20.5602240896358543No Hit
ACTGAATCAGGAACTCTGGGGGTGGAGCCCAGGCATCTGGGGCCCAACAA20.5602240896358543No Hit
GGCAGTAGAGGCTGTTGATGGACAAGTCTGAGATGAAGTGCTCGCAGGCT20.5602240896358543No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.5602240896358543Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGAATGTATGGATGCTTATATACCTTACTGTTTATTCTGAATGAAGATAA20.5602240896358543No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCGCTGCTTCCCA20.5602240896358543Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GTAAACATAACTTCTATATGCACTGGGAAACCAAAAATTTTGTGTGCAAT20.5602240896358543No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATA20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA20.5602240896358543Clontech Universal Primer Mix Long (96% over 25bp)
GACTCCAGCTCTCGCAAACCTTCGGCCCGATCAGCCCAGGCTTGCGATGC20.5602240896358543No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.5602240896358543Clontech Universal Primer Mix Long (96% over 25bp)
CAATTTTTCCAGGCAATGCCATCCATCGTCTGCTTCAAACCTTCTTTGGG20.5602240896358543No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.5602240896358543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCAT20.5602240896358543No Hit
TCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGGCGTGATGGTGGGCATG20.5602240896358543No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GGTGGACAGCGAGGCCAGGATGGAGCCGCCGATCCACACGGAGTACTTGC20.5602240896358543No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.5602240896358543Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTTCTACCAGAATGAGGTAATGCAAATAAGGCTGAGTCAATACAATG10.2801120448179272No Hit
ACGATGGAGGGGCCGGACTCGTCATACTCCTGCTTGCTGATCCACATCTG10.2801120448179272No Hit
GTCCAAAGTAGTCCAGAGAGTACATATAGTGCTCCAACTGTACCTACGTA10.2801120448179272No Hit
CAGCACTCCTACAGATGTCACAGCCATACACCTGTAGCCCTGGAGAGGCC10.2801120448179272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCA10.2801120448179272No Hit
GCATTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACA10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGGAAGGAAAAGGGTCTGTGCCATTCAAAGTGTGCTCTTTTTGCGGA10.2801120448179272No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACATTGGAAGCAG10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10.2801120448179272No Hit
CCCAGCTACTTAGGAGGCTGAGACAGGAGAATTGGTTGTTCCAGGGGGGG10.2801120448179272No Hit
CACTTCTTCCACCCCACTTCTTCCTTCACCAACATGCAAGTTCTTTCCTT10.2801120448179272No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCTTGTACTCTGCGTTGATACCACTGCTGTCTCTTAT10.2801120448179272Clontech SMART CDS Primer II A (96% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GACCCAGATGGTACTGCTCTAGCAGACTTTTCTGGTACTCTTCAGTCAAC10.2801120448179272No Hit
ACGCAGAGTACATGGTAAGATGTGGTAACAACGCAGGGTACTGGTAACCC10.2801120448179272No Hit
GGTCGGGCGCGCTCGCCGAGAGCCGATGTGGGGGAGGCGTCGTTTAGGGT10.2801120448179272No Hit
ACACATTTAATGCAGTGTGTAGAGGGAAATATATAGTACTAAATGTCCTC10.2801120448179272No Hit
GTCACAGCCAGGCATGGTGGCTCACACCTGTAATCCCAGCATTTTGGGAG10.2801120448179272No Hit
GAGCCACTGTGCCCGGCCCTGGTCTCAAACTTCTGACCTCAAGTGATCTG10.2801120448179272No Hit
GCCCCATCCCGTCCCGCCGGGCACTCGGAGGGCAGCGCGCCGGAGGCCAA10.2801120448179272No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GCGGCTCCCGTACTAGCTGCTCGGGGAGCCGGGCCAGCACCGGCTTGGGG10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCCTCACCCAAACTACGGTGGGATCCGGTCTGCGTCCCGTTCGCCGCG10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACCATTGGTA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTCATCAATTATCCAGTTTATATTTAAGCTTCCTTAACTTGCTCCCCAA10.2801120448179272No Hit
GCATTCAGCATGGCTTGGGAGATGGTTTCAAACAGGTGATCCGGATCCAT10.2801120448179272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACAT10.2801120448179272No Hit
GTATGATATCAGCTGTGGGTTTTTTTTGTTTGTTTGTTTTTTTGAGATGG10.2801120448179272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAAAACA10.2801120448179272No Hit
CTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCC10.2801120448179272No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCGATGCTTCAAA10.2801120448179272No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.2801120448179272Clontech SMART CDS Primer II A (100% over 21bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTACCTGTCTCTTATA10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTGGTCAGACGCCGCTGAATCAGCTCTCACCGGAGCAATTAAAAACC10.2801120448179272No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA10.2801120448179272No Hit
AAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCTTGCGCT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGGCTCGGAGATGTGTATAAGAGCTGTCTCTTATACACATCTGACGCT10.2801120448179272No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGATTTTCCGAAACCGGTTTGCAGATGCCCCCGGGGGAGGCTAGCGTTT10.2801120448179272No Hit
TTCCCATGTACACTGCGTTGATACAACAGCTTCACAACTACTCAGCCTTG10.2801120448179272No Hit
TTAAGGCCTAGCCTTAGAAGTCATAGTATCATTTGTGCTGCATTCTATTC10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.2801120448179272Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATATTACTGCATTACTCATACAGCAATATAGTCCCATGTACTCTGTATCA10.2801120448179272No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.2801120448179272Clontech Universal Primer Mix Long (96% over 28bp)
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GGAAAAGGGAGGGGAAATCTGAATAGAGACAGAACTCTGAATTAATGAAA10.2801120448179272No Hit
GTGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTTCTTCCC10.2801120448179272Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCCTG10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACTGCTTC10.2801120448179272No Hit
GTTCCCTCTTTACTCTCACTCTGTTCTGCTGCTAAAAGCAACCTTTAGAG10.2801120448179272No Hit
GACGCACACTTGGGAGAATTTCTTCGCGATGGCTTTGTAGATTTCCCAGT10.2801120448179272No Hit
GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAATAATTAA10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCTTTTTAAC10.2801120448179272No Hit
TCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAGCCTCACATATAAATAAGGACTTGCGACCCAGAATCACATCCAGA10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAATAAATAAAAGTTGTTTAAAAAA10.2801120448179272No Hit
AAGCAGAATTGGGGCTCTCTGAAAGGCACTTGTGAGGCATAGAAGTAAAG10.2801120448179272No Hit
GCTACTACTGAGCTAAAACTAGGAGAGCATATTAGAAATGACAGACTTAG10.2801120448179272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
CTACAGGAGGGCCTTGAATGTCCAACTAATGAGGCTGTAAATAATGCAAC10.2801120448179272No Hit
CTCTGAATCTCATGCAGGATTTCACGTTGCTCCTGCTCTTCTTTCCGCTT10.2801120448179272No Hit
GACCTAGGACTCCAAAAATGTTTCCCCTGAAGGACGCTGAAATGGGCTGT10.2801120448179272No Hit
ATTGGTTCAACTATTCCTTATTTCAAGCTACAGTTCATTTCACCTCTGCC10.2801120448179272No Hit
CTCATGGGTAATAACCAAGATGGAGTCCCTTTGGCAAAACTTAGGCATGC10.2801120448179272No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGGGGTGGATTTCTCAAGAACTATCTCCTTGCTAGACTGACAGTC10.2801120448179272No Hit
TCCTAATGATTAATGCAGTATCGAATGGACAAGTCCGAGGTGATAGTTAC10.2801120448179272No Hit
CTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTATGATTTTACTGGACTTTTTTTAAAGGGAGAAAGGGGGAGGAGAAT10.2801120448179272No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGCGTTGTAAC10.2801120448179272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATAGTTCCAGCCGAGCTCATAAGATTTTGCTCTCAAATTCTTTTTGGG10.2801120448179272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
GTCAAACCCTTGTGTCGAGGGCTGACTTTCAATCCCATGTACCTGTCTCT10.2801120448179272No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCGCTAGGA10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG10.2801120448179272No Hit
ATTAAAGACATAGCGTTAATCAGCAGGAAGAAAAGAATAAGACCCAGAGC10.2801120448179272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATCAGTGGTAAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10.2801120448179272No Hit
GTATGACGAGTCCGGCCCCTCCATCGTCCACCGCAAATGCTTCTAGGCGG10.2801120448179272No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAGCG10.2801120448179272No Hit
CACCCAGCTTCTGAGTGACAGAGTGAATATAAACCCAGCCTGCTCACTCC10.2801120448179272No Hit
TCTTCAGTCCTGCTCCAGATAACTTTGACCAGGAGCCTATACTGGACTCT10.2801120448179272No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.2801120448179272Clontech SMART CDS Primer II A (96% over 26bp)
GCCAACGTCATCGCGGGTGTATGGCTGGAGGAGGCGGGGCAGAAGCTGAG10.2801120448179272No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10.2801120448179272No Hit
TGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGC10.2801120448179272No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAATGGCGTTTTAAAC10.2801120448179272No Hit
GGCTGGAACCCAGGTGAACGAGGGCTTCCAGCCACTGGTGGAGGCACTGT10.2801120448179272No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT10.2801120448179272No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACT10.2801120448179272Clontech SMART CDS Primer II A (100% over 26bp)
CCTTCACATCCCTTGTAAGTTGGATTCCTAGGTATTTTATTCTCTTTGAA10.2801120448179272No Hit
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
ATATCAATTTGACTCTCTATGCTATCATGGACGAACAGACTAGGCTTCGG10.2801120448179272No Hit
GATTAATAGGCTTAAATTGCCAGGACTTTAGATAGACATTACCACTTCCA10.2801120448179272No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTATAACAAATATA10.2801120448179272No Hit
GAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
GCCGCAGCCACACAAACCGCCTAGAGTGTGCCAAACTTGTGTGGGAGGAA10.2801120448179272No Hit
CTCCACAAATACCTGGAGAAGGGGAAGGAGACGCTGCTTCACCTGGAGCC10.2801120448179272No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10.2801120448179272No Hit
ATGGGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAAAAAAAAAAAAAAAAAAAAAATTACACTGCGTTTATACCA10.2801120448179272No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.2801120448179272Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
GCTTTGGAGGTGATGACATCGTGCTCCACCCTGAGCAGAGGGAGGAGCAG10.2801120448179272No Hit
CTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCGGCGTTGATACC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GAATCATTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT10.2801120448179272No Hit
GTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTG10.2801120448179272No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ATTTGAAAGCGCCCTAAAATTTTGATGGAATTTGGTGTGAGAGGAAGTCC10.2801120448179272No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATGGTCTCGATCTCCTGGCCTCGTGATCCACCCACCTCGGCCTCCCAA10.2801120448179272No Hit
GTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGT10.2801120448179272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAA10.2801120448179272No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC10.2801120448179272No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAAAATGCGTTGTAAC10.2801120448179272No Hit
CATGGGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT10.2801120448179272Clontech SMART CDS Primer II A (100% over 24bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCTCTTAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTT10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA10.2801120448179272No Hit
GGTCAGCTCGTCGGCGTATTCCAGGTCGCCGCCCACCGGCAGGCCGCTCG10.2801120448179272No Hit
CTGCTTCCCATGTACTCGGCGATGATACCACTGCTTCCCATGAACACTTC10.2801120448179272No Hit
CCCAGCTACTTAGGAGGCTGAGACAGGAGAATTGGGGGTTGCGGGGGGGG10.2801120448179272No Hit
TAGTCCATGGCGTAGAAGCCGTCGCCGCCCCATCTGCTGCAGTCTGTCTC10.2801120448179272No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.2801120448179272Clontech Universal Primer Mix Long (95% over 21bp)
CACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGC10.2801120448179272No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGC10.2801120448179272No Hit
TCTGTACAGTTAAAGATATTAAGGCAGAACCTCAGCCACTTTCTCCAGCC10.2801120448179272No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.2801120448179272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGA10.2801120448179272Clontech Universal Primer Mix Long (96% over 27bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGAGTAAACA10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAAAGG10.2801120448179272No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGG10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTGAAGTCTCGCTCCTGAGGCTGCAGAAGAATAGATGGCTTTTCCCT10.2801120448179272No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAAACATG10.2801120448179272No Hit
CTGGACTCCCGTGAGCTGGAAGGAACAGATTTAATATCTAGGGGCTGGGT10.2801120448179272No Hit
CCCAGCTACTTAGGAGGCTGAGACAGGAGAATTGGGTGTTCCAGGGTGGG10.2801120448179272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC10.2801120448179272Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)