FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGG24.444444444444445No Hit
GCTCAGCGCCAGGCAGCGCTCCACGGCCATGGCAAAGAGGATGAGCATGG24.444444444444445No Hit
CTTTCGCCAGCGCCATCGCCATCTGGCGCGGACGCGCCACCGAGCGGGAT24.444444444444445No Hit
GCGGGACTCGTCCAGCGCCCTCGTGCCCGCCTCGACCCTCAAGCTGCTCA24.444444444444445No Hit
GCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGGGCGGACCGCCCGCCCG24.444444444444445No Hit
CTCCGATCGTCGCCGCCGCACTCGGCTAGAGTCCTCCGGCTGGTGGGGCC24.444444444444445No Hit
CCATCATGGGAATCATCATCAATCCAGCTTCGGTGGCGTTCTTGCCTGTC24.444444444444445No Hit
CTACTGGCGCTGGGCTACACCGTCGTCATTCCCGACACCGAGGGCCCGAA24.444444444444445No Hit
ACCTGAGCCTGGGAAGTTGAGGCTGCAATGAGCTGAGATGGCACCACTGC12.2222222222222223No Hit
CTGCAAGCTCCGCCTCCCGGGCTCACGCCACTCCTCCGCCTCAGCCTCCC12.2222222222222223No Hit
CCCATGTACTCTGCGTTGATACCACAGCTTCCCATGTACTCTGCGTTGGT12.2222222222222223Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT12.2222222222222223No Hit
GTCGGGGGCTGTGCGGATGGACACCAGGTGCTGCAGGCCCCCTGCGCTGC12.2222222222222223No Hit
AAGTTTGGAAGAGGCAGAGAAATCCTGCTCTCCTCGCCTTCCAGCAGCTT12.2222222222222223No Hit
TCTTTGCAGCAGGGCTGTTTTGTCTATATGATGAAAACGAGAAACAGCGC12.2222222222222223No Hit
CCCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATCACCGGCGTG12.2222222222222223No Hit
TCCTTACCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTTCTGCAA12.2222222222222223No Hit
CGGCCCTGGCATCTCCTCTTGGGGCACTGTCTCTGGCATCCGGCTTTCCT12.2222222222222223No Hit
ATAAACATCCCTAGTGCTTAAATCCCTGCTATGTGCCAAGCATGCTGCAA12.2222222222222223No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAACTGT12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
AATTACGATGGACTACAAAGGAAGTTATCTGCGAATCTGCTTGCTTCATC12.2222222222222223No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT12.2222222222222223No Hit
TGTGTGGTCTCAGCTACTCAGGAGGCTGAGGCAAGAGGATCACTTGAGTG12.2222222222222223No Hit
CCCCCAGATAGTCGATTTACCGATGCCCGCCCTGGCCGATGGCCCGCGTC12.2222222222222223No Hit
ATACCAGGGCATTTCTGAGGATGTTTTTGCCCCGGGCCACCTCACTCTCC12.2222222222222223No Hit
CTTCTCCACAGAGGTACGGCGGCATACAGAACATACTCAGATACCTGTCT12.2222222222222223No Hit
CTTCAGTACTTTTGCTGCACTGCTGCAATATGCATCTTTAATCAAATACC12.2222222222222223No Hit
CCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCA12.2222222222222223No Hit
GTCTTTATACAGCTTACTTTAAACATTATTTTCTATATCTTATATAAAGT12.2222222222222223No Hit
GTACTGCCGGGCCGACGAGGCGCGGCCCAACACCATAGATTTCGGCAAGG12.2222222222222223No Hit
ACCATCAGTTTCTTCTTTAAGGTAGACATTTTATGACGTAATGAACACCC12.2222222222222223No Hit
GATGATAATTCAGTAGGCTAGCACAAATTAATGGAAATATCTCTTAAGAT12.2222222222222223No Hit
CCACCACACCCAGCCAACCTTTGCATTTCTACTAGAGATGGGATTTCGCC12.2222222222222223No Hit
TTGATCAGGAGAACGTGGTTACTAGCACAGAGAGTTCTCCCAGTAGGTTA12.2222222222222223No Hit
CCGACAGCACCACCGATCTCGTCAACCAGGAAGGCCGCCCGGCCGCGCAG12.2222222222222223No Hit
GAGAAGAAGAAGCGTCCCAGTTTTTTGTCCACCTTTGCCTTGGTGGTGAC12.2222222222222223No Hit
GAAAAATGGAAAATATTAGTCCCTTATGTAGTTCTTTTCTCAACATGACT12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)