FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGGACCTAAACCATGTGCCTCTGCCTGTTGGGCTACGGCCCCGCGG24.444444444444445No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA24.444444444444445No Hit
GCCTTCGCGGTGCTGGTGACCGGACTGGCGGCCACCGACCTGCTGGGCAC24.444444444444445No Hit
GCAGTGGCTTTTGTCTTCGTCGAAAAGCGTGCCGACGAACCGGTGATTCC24.444444444444445No Hit
GGAAGGGGCGCTGAACCGCGTCATTGCCAATGCCAACTTTACCGGACGGG24.444444444444445No Hit
GGTGACCAGCACCTCATCCCCCGTGTACTGCCGCAGCACGGCCGCGGCGG24.444444444444445No Hit
GGGTTGACCAACAGACCACCCTGGGCGGCGCCGATCAGGTCCTCGTTGAT24.444444444444445No Hit
GAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT12.2222222222222223No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCGCTAAGAAGT12.2222222222222223No Hit
CACTGAGTGTGTGCAATTTTTATTCAAGGTCATCGTGATGCTGAGAAGTT12.2222222222222223No Hit
ACTCCAGCTTGGGCAATGGGAGTGAGTCCCTGTCTCAAAAAACAAAAACA12.2222222222222223No Hit
ACAGTGGGCAGGCGGCGCGCATCAACGGGACCTTCCGGGCTTGTGGTCGG12.2222222222222223No Hit
GGTCACACAGTGACTGAGCCAATTCAGCCACTAGAGCCTGAACTGCCAGG12.2222222222222223No Hit
ATTTAAAAAAATAAAAAGATGGAAACAGATAAGTAGAGAAGGTCTATGTC12.2222222222222223No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12.2222222222222223Clontech Universal Primer Mix Long (96% over 26bp)
TCCTCGGTGCGCGACCCCCGGCTCAGAGGACTCTTTGCTGTCCCGCAAGA12.2222222222222223No Hit
GTATCTGAGTATGTTCTGTATGCCGCCGTACCTCTGTGGAGAAGCTGTCT12.2222222222222223No Hit
ATCCATCTGTGCTTTCTGAAGCAGTTCAATTGTAAGAGAAAGCCAATCAG12.2222222222222223No Hit
GCTTTAGGTGATCATTTAAAGCAATCTGCATGCCACTTTTCTTTCTCAAC12.2222222222222223No Hit
ACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCGCTAAG12.2222222222222223No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCCATCAATCTGTCAAT12.2222222222222223No Hit
ACACTGTGCTAGCAGGCTGAACAATTTTGCGGGAATATGGGAATTTTTCT12.2222222222222223No Hit
ATCACGCAGAGTACATGGGAAAGGCGAGAGCTGCGAAGGGCCAGGTGTCG12.2222222222222223No Hit
CCCTCCTGTCAGTTTCACCAATCACATGCCTTTGGCTGTGCTGCACTATT12.2222222222222223No Hit
GCTTATTACAAAGATACATGGCATAAAAGAAGACCAGGGGCCGGGTGCGG12.2222222222222223No Hit
GTACCTTTCTTTCTCTAGAAATGAAGCTGTAGCTAAGTGACACCAAAACA12.2222222222222223No Hit
GAGATGTTATATGTGGAGGTGCTTAACTGTGGGTTAGGGTTAAGTCCTTT12.2222222222222223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATT12.2222222222222223No Hit
CCCCAGCATCCAGTCCCGGACCATGCCGGTGGAGCAAGTGTTTGCCAAGA12.2222222222222223No Hit
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGT12.2222222222222223Clontech Universal Primer Mix Long (95% over 21bp)
ACTATCTCCAGGACAGCTTGACAGTGCACATTTGAGCTTCCGTTTAAGGG12.2222222222222223No Hit
CCCTGTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCACGCC12.2222222222222223No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT12.2222222222222223No Hit
TGGTGAACCTGGATCAGGGCGTGGTCGTCTTCATGAGCTCCATTCTCGGC12.2222222222222223No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACGTGTGCCTCTAAGTAGT12.2222222222222223No Hit
CTTCCTCCCCACCTTCTTCCTCTTCTTCGTCTACCTCATTGTCAGCCTCC12.2222222222222223No Hit
TTGCTGGTCCATGCGCGGACCTTGATGTCGTAAGTGGTGTCTGGCTTGAG12.2222222222222223No Hit
TGCCATCATCGGCCACTATGACTGCACTTATGGTGGTGGCGTGCACCTGT12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)