FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATGATTCAGTTACCTCCCACCAGCTCGCTGCCATGACACTTGGGG32.912621359223301No Hit
AGTTAGGAAAATATCAGAGCTCTGAGAAAAGGGACAAAGCAAGTGTTTTG32.912621359223301No Hit
CAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCAGAGCGAAA32.912621359223301No Hit
ATCTGGAAGGCTGAGAATCAGGTGACTGCTTAGAGAGGCCTTGATCGTTC32.912621359223301No Hit
GATGGGTACAGTGTGGGTGACCCCGTCACCGGAGTCCATCACGATGCCAG21.9417475728155338No Hit
GCGCGTGCCCCGCCGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAA21.9417475728155338No Hit
GTTCAAGTACTTATCGTGCCTCAGCCTCCTGAGTACCTGGGAATACAAGC21.9417475728155338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT21.9417475728155338Clontech Universal Primer Mix Long (96% over 26bp)
CCTTAATCTAGTTTCAGTATGCAGGATGGATCTGAGGAGGAAATGTGCGT21.9417475728155338No Hit
GGAGTTCAAGACCAGCGTGGCCAACATGGTGAAACCCCATCTCTACTAAA21.9417475728155338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA21.9417475728155338Clontech Universal Primer Mix Long (95% over 22bp)
CATATTGTTTTTGCTGATGATGAGAGTAATGAAAGAACAGCACATCCATA21.9417475728155338No Hit
TTATTAAAGAAGTCAGGATTATTACAAGTTTTCTAAATTTTTTTTCCGTT21.9417475728155338No Hit
ATTTAGTACTTGACCTTTATCTTCTAACCATTCTTTTATGTCATCAAGAG21.9417475728155338No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGCGTCAAATTAAGCCGCAGGC21.9417475728155338No Hit
GTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAAC21.9417475728155338No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21.9417475728155338Clontech Universal Primer Mix Long (96% over 26bp)
CTACAGTGGTGAAGAGACCAGGAGGCAGCTCTCAGTGAAACCAACATTGC10.9708737864077669No Hit
TCCCGGGTTCAAGCGATTCTTGTGTCTCAGCTTCCCGAGTAGCTGGGATT10.9708737864077669No Hit
GTAACAGAGGTGCTGGGTCAGCCAGGCAACAACACAGGAGACGCCTGTAT10.9708737864077669No Hit
GAGTACTTTTTTTTTTTTCCCAAAACAATTTATTTATTTATTTGTCCTCT10.9708737864077669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGT10.9708737864077669No Hit
CCATTCGATTCCGTTCAATGATTATTCCATTCGAGTTCATTCGATGATTC10.9708737864077669No Hit
AAGTCGACGAGGAGGCGATTGACGCGTTAGCGCTCGCGAGCTCTTCCGGC10.9708737864077669No Hit
AGCAAGCAGAATTTGGAATTCATCCAATCCAAATGCGGCATCTTCAAACC10.9708737864077669No Hit
ACTCATGTCAGAGGCGTATTAATATATTTTCAGGTTAAAAGCAGGTGAAA10.9708737864077669No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG10.9708737864077669No Hit
CTTATTTTGAGGCTCTTCCTTCCTTCTCCAATGGGACCAAGAGCCCCAGA10.9708737864077669No Hit
CTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGAT10.9708737864077669No Hit
CATGAGGAGAGTGCTAGTTCATGTGTTCTCCATTCTTGTGAGCATCCTAA10.9708737864077669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9708737864077669No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.9708737864077669Clontech Universal Primer Mix Long (96% over 26bp)
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC10.9708737864077669No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAGAGTACATGGGC10.9708737864077669Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC10.9708737864077669No Hit
ATCTAGCACCCCATTCTAACCTTAATGTCTTAAAAGCTAGATGTTGCTTG10.9708737864077669No Hit
CATCAGTAGGGTAAAACTAACCTGACTCACGACGGTCTAAACCCAGCTCA10.9708737864077669No Hit
CTCTATAATCGGTGACACCACAACAATGCAACGTATTTTGGATCTTGTCT10.9708737864077669No Hit
ATAGAAACTCAGATTTTCAAGCTAGAAAGGACCTTTGAGATCATCTAGGC10.9708737864077669No Hit
GCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGAT10.9708737864077669No Hit
GGGGAAAGAGACTTTCGCACTATATCCCAGAGTCTCTGCTCCTGCTTCCA10.9708737864077669No Hit
GGCTATGCCACTGCTCCACGGCTGTGCGGTGCGTGTTGAGCATGCGCCTC10.9708737864077669No Hit
CAGCATAGATTTGAAATACATTGTGTATGTTTTGGGACAAAAGCAAACAT10.9708737864077669No Hit
CTTCTTTTGCTTACCCGTTGGAGATGTGCCCCACACCTCGCCATGATGGC10.9708737864077669No Hit
ATGCGATGATTCCATTCCATTCCATTTGAAGATGATTCCATTCGAGATCA10.9708737864077669No Hit
GTTCCCACCAACCATTCATTCATTTGCTAACCCGGCACTTTCTTGTTGAG10.9708737864077669No Hit
GTTGTAAGCTTGGGGTATGAAGGTTTGGGCCACGCCTGGGCGCTTCCGGC10.9708737864077669No Hit
GCAGTACATACTGTACCTGGTGGAGCATGAGGAGTACCAGCTGTGCCAGC10.9708737864077669No Hit
CTCTGACCCCTGCCCGTGGAGCCCAAGCACTGAGCAGGGAGGTGAACGCC10.9708737864077669No Hit
CCCGTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCATTCGTA10.9708737864077669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAG10.9708737864077669No Hit
GCAGAAAATTGCATATAACCTAGCCAAGTCTCCCTTCAGAGTTCTTAGCC10.9708737864077669No Hit
ATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA10.9708737864077669Clontech SMART CDS Primer II A (96% over 26bp)
TTTTGAAAAAGCTTAACTTAACAATTTCTGATGTCTATCTTTTAGAGTTC10.9708737864077669No Hit
AATGGATGGTCTTGGATGATGGATAAATAGGGACAGGGACAGTTAAATTG10.9708737864077669No Hit
ACCTAAAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTA10.9708737864077669No Hit
TTCTTCACCAGCTTCTTGTTGACCAGGAAGGCGATGTTGAGCGTGTGGAT10.9708737864077669No Hit
GTCTAGCAGCTGATGTGGGTAAAGCTGGAGCTGAAAGGGAATTCCGAGGC10.9708737864077669No Hit
CCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA10.9708737864077669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTTT10.9708737864077669No Hit
TTCCAATACCGCACCTGTGCTGGCCTTTCTCAGGGGCTCCAGGAGCATTT10.9708737864077669No Hit
GGGCTGGACACAAGGTCGGTGGCCCAAAAAGAGTGCTGAATTTTTGCTGC10.9708737864077669No Hit
GTTAATGCTATGAAAAACAGTGAACTGATTTGATATGATGGAGAGGATCT10.9708737864077669No Hit
GAGTGCTTCAACAAGATTTCGAGAGACGCTGACTGTCGGGCGGTGGTGAT10.9708737864077669No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTAGACCGT10.9708737864077669No Hit
CATAAAAGTCATGAAGAAAACATAGGGGAAACACTTCAGGACATTGATCT10.9708737864077669No Hit
GCCCAGGTGCAGGCCCAGCCATGCGCCGGCCGGCACTCCCAGCAGCGTGG10.9708737864077669No Hit
CTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGA10.9708737864077669No Hit
GACATGACCAGCTCATCCCCTTTCCGCAGACCCGCAATCGCGCTGCTGAC10.9708737864077669No Hit
AGGACGACTACTTCGAGCTTCTGACCAGTCTCTCGGTCGCTGGTTTGGAT10.9708737864077669No Hit
ATGTACACATGGGCACTGCCCTTACAGCTACACTCTAAGAACAGGTTGGC10.9708737864077669No Hit
CTTCAAGGAAGTTAAGGACATATTTTATCTCAGGCCTCTGTGTGCAGAAA10.9708737864077669No Hit
ATCCTTAATCCAGTGACCTCAGTAAACTTTTAAATATGCAAATCTCATTT10.9708737864077669No Hit
GCCAGATTCAGAGGAAAGAAGCCAGGGTAGAGTGAAAGGTATTAGGCAAC10.9708737864077669No Hit
ACTGACACCTGCACCCATACAGGTGCTGAGGGAGAGCTAAGGAGCCACAG10.9708737864077669No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC10.9708737864077669No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG10.9708737864077669No Hit
ATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGA10.9708737864077669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT10.9708737864077669No Hit
ACATTGGCCTGGTGTTCCCCCTGAGCTGCAGTTTCTCTTTGGGTGATTTC10.9708737864077669No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCAAGGTAATCCTGCTC10.9708737864077669No Hit
CTCTTACCTCGCCCTGGCCACCGAAACCGTGGACATGTTCCACATCCTCA10.9708737864077669No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)