FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTTATCTCAGTTGAGATTTTGGACTTTTGGGTTAATGCTGGAATGAG32.912621359223301No Hit
GATTTCTACACTCCTGAAAAAGACAACAACATCAAAAAAAATTAAGAAAT32.912621359223301No Hit
GTGGCTACTCACAGTGTCCAGTTGAAGTAACAGTGGTTCAAGTTCTGGTC32.912621359223301No Hit
AGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTT32.912621359223301No Hit
GGGTACCTTTGGAGATAATGATAAAATTCAACAGGTTGAACCGTCTAACA21.9417475728155338No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG21.9417475728155338Clontech Universal Primer Mix Long (96% over 26bp)
CACCTGTAATCCCAGGACTTTGGGAAGCCGAGGTGGGTGGATCACCTGAG21.9417475728155338No Hit
CTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTC21.9417475728155338No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT21.9417475728155338No Hit
GTGTAACAGTTGGGGGATGGGCCTGCTGCTCTGATCCGGGACTTCCCGAG21.9417475728155338No Hit
TCTCGGCTCACTGTAACCCCCGCCTCCCAGGTTCAAGTGATTCTCCTGCC21.9417475728155338No Hit
ACTCATGCTTGAAGTTCTGAAATTTGGGAGCGTTATGAGGTTTCCATGCC21.9417475728155338No Hit
GGATAGGAACGGTACGTACAGCAACTCCACTTAATGGGATTTTCCTGTTT21.9417475728155338No Hit
GAAGAAACCCTGAACTGATGCAACCACCACTTGAAAATAAAGTTCCAAGC21.9417475728155338No Hit
CCCCAGGCACCAGGGCGTGATGGTGGGCATGGGTCAGAAGGATTCCTATG21.9417475728155338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT21.9417475728155338Clontech Universal Primer Mix Long (96% over 26bp)
TCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATC21.9417475728155338No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG21.9417475728155338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAA10.9708737864077669No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.9708737864077669No Hit
AGGCAGGCCAGATGGCCCCTCCACTGGGGAATGCTCCCAGCTGTGCTGTG10.9708737864077669No Hit
TCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT10.9708737864077669No Hit
GCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCT10.9708737864077669No Hit
CCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCG10.9708737864077669No Hit
GAGTGTACCAGGTCTGTAGCAGAAAATAATCAACTAAATGTCAGGGACCT10.9708737864077669No Hit
GGTATCAACATCAAACGGAAAAAAACGGAAATATCGAATGGAATCGAAGA10.9708737864077669No Hit
GTATCTCCCCTGTTTGGCAGTTTACCTTCTCTTTTTGGAAATCAGTGATT10.9708737864077669No Hit
GTTCAGATCACATGCCCATTTTCTAATGGGATTATTTGTTTGTTTGTTTT10.9708737864077669No Hit
CACAAAATGCCCAAGGCACTCACTGGGGAGGAAGCAAGGAAGCAAACAGT10.9708737864077669No Hit
CCTAAGGCGAGCTCAGGACGGACAGGAACCTCCCGTGGAGCAGAAGGGCA10.9708737864077669No Hit
GGCGTTATCCCGAGCCTCGTCCATGCGCACTGCATTCTCCGGGGCGGAGA10.9708737864077669No Hit
TCCTAGAACATACAAAAGAACATGGTGGACTTTCAGGGAGTGCAATGGAA10.9708737864077669No Hit
CCATAAGATACTTGAAACATTTCAAACATATTATTCAGATGAACCACGAG10.9708737864077669No Hit
GTCCTGGGAAACGGGGCGCGGCTGGAAAGGCGGCCGCCCCCTCGCCCGTC10.9708737864077669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTAA10.9708737864077669No Hit
CCCGATGACCTTGGGCAGGCGCTGCAGTGTTCCTACATCGGCAGCCAAAC10.9708737864077669No Hit
CATGGAAACCTAAAACGCGATGAGATTAACCCCAAAGAAAGTAAAGGAGA10.9708737864077669No Hit
AAATTGGCTTCAACCCAGGAAACATTTGTTACATTTTACAAGCAGGTCTT10.9708737864077669No Hit
CGTCAACACGTCGTGCAGCGTCTCGGCCGCCTCGGCGGTGAGCGCGTTGC10.9708737864077669No Hit
ATGACGATGTGAGTGTTCTTGGGATCCGGAAGCATTCCCTTTGCAGTGTC10.9708737864077669No Hit
TTCTTGCTTGGAAGCCAGGTACAGGAGAGGCAGCATGCTTGGGCTGACCC10.9708737864077669No Hit
CTGAATTGCAGTGTACCAGTTTGAGTTGAAACGGTATGTGACTTCCCCAG10.9708737864077669No Hit
GTATAAAGGTTTCAGGTTTATTGCATTAATTGGCTCTTAGAGTTTTCTCC10.9708737864077669No Hit
AGCGAGGCCGGACTGGACGTGAAATCGTTTGTGACGTCGCCTCGGTTCAT10.9708737864077669No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.9708737864077669Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.9708737864077669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTAAAAAGGGTCTTGCCCTTGAATAACTAAGTCATACAAATAGAAGGA10.9708737864077669No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTA10.9708737864077669No Hit
ACAGCACCCATAAGTGAAGAGGTAATGCCACCAAAGGAAACCAGAGTGGA10.9708737864077669No Hit
GCAGCAGCAGCAGCAGCAGCAGCAGCCGCCGCCTCCGCAGCCCCAGCGGC10.9708737864077669No Hit
GAGCAAAATGATAGGACCACCCTGGGCCCCAGCTCTGGCTTTTACCATAG10.9708737864077669No Hit
GCGCTCGGGCCAAGCGCACAAGTAAAACCCCCAAAACCCCACCACCCAAC10.9708737864077669No Hit
CAGCAATGGCTTTCGCGAACCGAGCACAGTCCAGCTGTAAGTAAATATCC10.9708737864077669No Hit
GATGGGAGAAGGGCTTGCAAACCAAGATCAAGGTGTTTCTCCGCTAATAC10.9708737864077669No Hit
GTGTGTTTCTGCATTTGCAAATACTGAAGGAGGATATGTATTTTTTGGTG10.9708737864077669No Hit
GGACACCACAGGAGGCACAGCCTATGGGGCGGGACGGGGAGGGGCAATGA10.9708737864077669No Hit
CCCTGCAGCCTCCACACCTACCACGACCTCCCAGGGCTGGGCTCAGGAAA10.9708737864077669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA10.9708737864077669No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA10.9708737864077669No Hit
CCGTAGGACAGGGAGGTCGGGAAAGTGCTGTGGAATGCCTCGACGTCGTA10.9708737864077669No Hit
GTCTTACATATGTGTGAGAATCAAATCAAATCAAATAATACATACAAAGT10.9708737864077669No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10.9708737864077669No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCCAAAAAAAAAAAAAAAAA10.9708737864077669No Hit
TCGCTGTTCCGCGACTTCAAGAAGCGCTACCCCGAGATCGAGCTGACCTA10.9708737864077669No Hit
GTGGCAGCGATGTCCTCCGATGGCGCAGAATCGCCGATCGAAAGAACCTC10.9708737864077669No Hit
CTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTACTACG10.9708737864077669No Hit
GATTGATACTGGGTTCCATGTAGAGGGCAGAATTTTAAGCCTGAGTGAAG10.9708737864077669No Hit
GTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAG10.9708737864077669No Hit
CGCTGTGACCTTCCCACCCCACCGCCCAGCCTGCAAGTCAGCAGGCTGGG10.9708737864077669No Hit
CTTCTCCTGTGGATTGTCATGGGCCTGAGGACTGTGAGTGATTTTGCCAC10.9708737864077669No Hit
CTCCTCTTCTGGTTTAGGAACAATCTGTTCCTTTTCCGTAAGGATCATCT10.9708737864077669No Hit
TATCTGACCTTCAGGCCTTCATCATTCATCAGAGGCCTCACCAGGCAAGA10.9708737864077669No Hit
GTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAAC10.9708737864077669No Hit
ACTCTGTTCTGGGGGACAGCCAAGTTGTTCTTTCAAGAAATAGCATGTGT10.9708737864077669No Hit
GTACTACACTGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGTG10.9708737864077669No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10.9708737864077669No Hit
CCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACC10.9708737864077669No Hit
ATACATTTCCATCCAATTGATTTTTGACAAAAGTGGCACACAGAATGGGG10.9708737864077669No Hit
ATCTAATGGAATTGCATGGAATCATCATCAAATGGAATCGAATGGAATCA10.9708737864077669No Hit
ACATACGTTTAAGGCTGCATATTGCAGGCGGTGCAGCTGTCTGGCAGAAG10.9708737864077669No Hit
GCCTGGAGAATTACTTTTCTCTTTTAAATGAGAAGGCCACCAATGTCCCC10.9708737864077669No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)