FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43.571428571428571Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTAAAGTGAAATTTTTAAGAAATAAAAGGCTGTTTTTTTTCTTTAGGT32.6785714285714284No Hit
GGTTTGCATGGAGTTGCTGCTGTCCAAGGCATCACCAAGATTAAAGTCCT32.6785714285714284No Hit
ATGCCAGGCTGGTGGATGGGCGCGCCGTGGACAGTGAAAAAATATATTGA21.7857142857142856No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTAT21.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATTATGGGTGGAAAACATTGGCTCTTCCTTGGGGAGTGATGCTGGGGA21.7857142857142856No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.7857142857142856Clontech Universal Primer Mix Long (95% over 22bp)
TTTCAGCACCTTGAAAGGACCTTTAAAGCAAATATTGTCAGTATGTTCCA21.7857142857142856No Hit
AGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG21.7857142857142856No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
TAGTAGCTTCTGCCACTTTGAGAGATGACAGACTTATTCCAACCACATGA21.7857142857142856No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21.7857142857142856Clontech SMART CDS Primer II A (96% over 26bp)
ACACCGGACCTTGCGCACAGCCCCCATCCAGCCCCGTGTCTGGGAGCCTG21.7857142857142856No Hit
GCCCGACACCGACAGCGACTTGAACGCGAAGACGGGCGCCTCGCGCAGCT21.7857142857142856No Hit
AATGAGAGGTCTGTTCCTAATGCCCCCACTAGCCTGAGAACCCAGCAAAA21.7857142857142856No Hit
TTCCCCAGTGACCAACAATTTTAATTCCAAGAGGTGAGGATCTCAGGAGG21.7857142857142856No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21.7857142857142856Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGACCTGCTGAAAAAAAAAAAAAAAA10.8928571428571428No Hit
TTTTTCACGTATTGAAAAGTGTCCAGTTTCTGGTATGTTTCTGTAACATA10.8928571428571428No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGACAGTGG10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTCAGTGGGACCGAAAATAAAATGTTATGACTTAAATCACAAGAGGCT10.8928571428571428No Hit
GAATCCAAGGAGCAAAACTGCGTCGGGTTTGCGGTGATTTGACCCCCCGC10.8928571428571428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGAA10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGAAATGGGAATGGAAAGAAGATACAATGCCATACTTACTAAAAAGAA10.8928571428571428No Hit
GTCATCTTCTCTCTGTTGGCCTTGGGGTTCAGGGGGGCCTCGGTCAGCAG10.8928571428571428No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTATTCTGCTCAGATGAAGCTAGTTCCTTGCTAAAATAAGAGCAAAT10.8928571428571428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8928571428571428No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT10.8928571428571428No Hit
GGTCACTAATGACCTCCATGTTGCTAAATTCAATGGTCATTTCATCTTAT10.8928571428571428No Hit
GTCTTTTTCTGTCTGTCTCTCTCTTTCTTTCTGTCTGTCTCTGTCTCTGT10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTTGGATAAGCGAGGTCAGTCAAGCGATTCGTTGCCTCCGGGTCCCA10.8928571428571428No Hit
AAGTTGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGA10.8928571428571428No Hit
TTAGATATTTTTGCAAAGCGGGCGTGAACGAGAGAAGGCAAACTTCAAAT10.8928571428571428No Hit
CTGTAACCTACAAGAGAAATGAGACTCTTTGAGTACCAGAGTGCAGTCTG10.8928571428571428No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTAACGTTGATCGTCCTTTAGTTGAAAACTCTCTTCTTGCTTGTGTG10.8928571428571428No Hit
GTATGTATGTAACACTCAGATGGAGAGGTTTTAAAACATCAAAGGGGAGC10.8928571428571428No Hit
GGTCACTAATGACCTCAATGTTGCTAAATTCAATTGTCATTTCATCTTAT10.8928571428571428No Hit
GATGCGGTTGATGCTGGCACACTCGTGGTTCCCACGGAGCAGGAAGAAGT10.8928571428571428No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTTAGCTGTGCCTTCAGGCGGTGCGTCTAGCAGGGCTGATGGGGGCC10.8928571428571428No Hit
GCACAGCTAAGTGGGTGTGATACTCTTCTTTCTGCAGTCTCTTGTCTATC10.8928571428571428No Hit
ATTTAATACAGTGAATTAGCAAAGAGAGGATGTGAGCTTGGCTTTTTATC10.8928571428571428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT10.8928571428571428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACAGGTTA10.8928571428571428No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGGGAAGCAGTGGTAT10.8928571428571428Clontech SMART CDS Primer II A (95% over 23bp)
CTAACAGCAGCTGGGAAAGAAGTTATGCTTGTTGGAATTGGTGACAAAAT10.8928571428571428No Hit
CACACGCACACTATTAGCAAGTGACATTGGATAGACAGGTGTCCTCTCTT10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGT10.8928571428571428Clontech Universal Primer Mix Long (95% over 22bp)
TTCAACATAGTCTTCCCAGATTTTCTCCCATTTTTATCGGAATAAGTTTC10.8928571428571428No Hit
GAACTATCTGAATTACAGCTTCGCCTTTTGGCTCTTCAGTCAGCCAGTAA10.8928571428571428No Hit
CCACCAGATCAGCAAAACGTAGCAGAATACGTTCACGCTCTGCGGGTAAT10.8928571428571428No Hit
CCAGTAAACTGATATCGGTGGGGTCAGGGCGCTCATCAAAATGCGCGCTG10.8928571428571428No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACAGTATGAAAAGGAAGGAAAAAAGATTAACTTTACAGAGAAGAAA10.8928571428571428No Hit
CAGGAAAAAAAGGGGCGAGGCTGGGGTCCTTTCACGGATGACAGTGCCTT10.8928571428571428No Hit
GTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATG10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCTCCACAGAGCTCACCTGCAAGATCTGCGTGCGGCAAGTGGAAGGGG10.8928571428571428No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GTACAGGGCTCCCAGCAGCGGCATCTGCAAAGCCAAAGCAGTTATTTTAA10.8928571428571428No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGGTA10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
TACTAATTTCAAATGCTTCCTGGTAAGCCTGCTGGGAGTTCGACACAGTG10.8928571428571428No Hit
ATGAAAAGATTGTGTGTCTATCCAACAGGGAGCCACAGTATTTAAATTGA10.8928571428571428No Hit
TGTTTAAAGAGGAAACCAGAAAACCCTTAAATTCACCTTTCTCTCTGGTT10.8928571428571428No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACAGGGTTTTGCCATGTTGGCCAGGCTGGTATCGAACTCCTGACCTCAA10.8928571428571428No Hit
GCTAATATTTATCATAAAAATCTGCAACTGAATGATTTCTCATTGAAGCC10.8928571428571428No Hit
AGGGAGGGAGGGACGTGAAGCCAGGAATGGCTGGTAAGCGGGTGGTAAAA10.8928571428571428No Hit
CCATCACATAGCCCCTCAATGAAGGAATTACAGTACTTTATAAAAATGTC10.8928571428571428No Hit
GTGCTTATGATAGGGTTTGAGGGAATGGGTGTGCTTCTCCTGTCTCTTAT10.8928571428571428No Hit
GCGTTGAGTCCGTCCGACTTCACTGCGCCCTTTCAGCCTTTTGGGTCCTG10.8928571428571428No Hit
GCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTG10.8928571428571428No Hit
CACTGGCCGTGATATCGGTGCAGTGCTTCACCACCTTCGCCGGCCGTCCG10.8928571428571428No Hit
AACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCA10.8928571428571428Clontech SMART CDS Primer II A (100% over 26bp)
ACCCAGACAAGGCTGGAGACAAACCTCATATGCAGACTTATGAAGTTGAC10.8928571428571428No Hit
ATTACATGGAACTTTTCTGTAATGGGTACAATGAAGAAGTTTCTAAAAAC10.8928571428571428No Hit
GATCTAGCCCCATTGACTCTTCTAGAAATGCAGTATTGCTTTGACCTGCC10.8928571428571428No Hit
TTCTTAACATTACATAATTATATTTGTCAGAATATGACATAGAAGTCAGT10.8928571428571428No Hit
GACCTCTGCTCAAGGCGATCCCTCCAAGGTAAGGGGCAATGCTGGTGGCA10.8928571428571428No Hit
GCTGGGTACCATGCAGGCAGTGATTAACCTGCAGTTACCGGTAAATATCG10.8928571428571428No Hit
GACAGCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGGTGACCACTGTGGTGCTCTCCTCAATCTGCTGAGACCAGTACTTG10.8928571428571428No Hit
GGTCTCGGCAGACTCTTTCATATACTCTTTTAATCTACCACCTCAAAGAA10.8928571428571428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)