FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32.6785714285714284No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32.6785714285714284Clontech Universal Primer Mix Long (96% over 26bp)
GGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTT21.7857142857142856No Hit
ACATTGCTTAATATTAATTTTTCATTAAAATTTGGCCTGATTGATTAAAG21.7857142857142856No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA21.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCAGAGCAAGCGTGGCATCCTGACCCGGAAGTACCCCATTGAGCATGG10.8928571428571428No Hit
GGTAATAATTTCGCCATTACCACGGCTACTTTGCAGTTGCTGATTCGGGT10.8928571428571428No Hit
GGGCAATTTTTTCCTCGGAGCTCAGGCTGCGGGCACTACTCATATACTCA10.8928571428571428No Hit
CATTGTCCCCTCTGCAGGGATTAATTCTGAATTATTTATGTACCTATATA10.8928571428571428No Hit
CAGCTAAATTATGATTTACGCAAAACCATGACTAAGGAATTTCTCTTAGA10.8928571428571428No Hit
GTGCATAAGGAAGAACTCTACAGCAGCCCAATCTTCAGGCTAGTGGGATT10.8928571428571428No Hit
CAGCTGGAGACAGAGATCGAGGCTCTCAAGGAGGAGCTGCTCTTCATGAA10.8928571428571428No Hit
CTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGATGGATCGAGGT10.8928571428571428No Hit
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT10.8928571428571428No Hit
CACTAATGGCAGCTGCACTCGGATATATTCCACTCAGTGACCCAGTTTCG10.8928571428571428No Hit
TCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA10.8928571428571428No Hit
CGGCAGTAATGGTACCGGCAGCGCGGCCACCCAGGTTGCTAAAGTCAGCA10.8928571428571428No Hit
GGATTGTACCCACCTACATGCAGATTCCCCAACATTCAAATCCATCTCTC10.8928571428571428No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
GGGTTATCGTCCGGAGGAGAGTTTCCTCAAACCTCCGATGTTAGGTTTTT10.8928571428571428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGGTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CTAGAGGACTTCCGCGCCACACCATTCATCGAATGCAATGGAGGCCGCGG10.8928571428571428No Hit
ACCTAGGCTCATGGACGAGATGGGAAGGCACAGGGAGAAGGGATAACCCT10.8928571428571428No Hit
GGGGGCAGGGGGTGGATCGCGGTGGACGTGGGATGCAGTGGGTGCTACGC10.8928571428571428No Hit
AGATAATTTCAATCACCTCGTTTGTGATGACAGCTTGGCGGGTACGGTTG10.8928571428571428No Hit
CACATTGACTAAAGGCCACACTTTATTCAGATTTTCTTAATTTTTACCTA10.8928571428571428No Hit
GTTCCAGGTCAGAGACAGCATATATTTTTTGCCCTGTACCAGGCCGCCGG10.8928571428571428No Hit
TCCAACAGTTTCTCGATCGTCAACACGGTCTTTATCTTGATGGTCGTCCT10.8928571428571428No Hit
GTGTGGGTGGTATGCGAAGTTTGGTGGGGTATGGCGGGATCTGGGGAGCT10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGG10.8928571428571428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGATGT10.8928571428571428No Hit
CCGTATCCTTGTACATTCATCATAATTGGGGGACATACTTAAACATCCAA10.8928571428571428No Hit
GGCTTATTGTGAGGATCAAATGAGTTAATACAAGTCCTTAAAATGGAGGT10.8928571428571428No Hit
GACAGAAGATAGGCACAGAGAGAGAGAGACAGAGAGACGCAGAAAAAGAA10.8928571428571428No Hit
ACCGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGATGTTCTG10.8928571428571428No Hit
TCACCACACAGTCTGCTGAGGTTGGAGCTGCTGAGACGACTCTCACAGAG10.8928571428571428No Hit
GCGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAGGTAGTCAGCGT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CAATCATCCCTCACAGAACCCACCATCACTCAAAGCCACACATATCAGGA10.8928571428571428No Hit
ACTTTCGATTGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG10.8928571428571428No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
AAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCC10.8928571428571428No Hit
GCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGGTGATACCACTG10.8928571428571428Clontech Universal Primer Mix Long (95% over 23bp)
GGTCCAGGTCAGAGAAAGCATATATTTTTTGCCCTGTACCAGGCCGCCGG10.8928571428571428No Hit
TTGAACCACTTTAATTAACGCTTTTAGTTTACATTTGGCCACCTCAAAGT10.8928571428571428No Hit
GGATGGAACATTACAGGACTCTATCGAGGCCCGGTAATTGGAATGAGTCC10.8928571428571428No Hit
GGGTGGAAGTGCAGGGCTCGCGGCCTGGCACGATTGTACAGCTGACAGAG10.8928571428571428No Hit
GGGAGAGGTCTTGCAGATCAATAGGAATAAGCAAATGAGACTGGATAATA10.8928571428571428No Hit
GATTTGTACTGTGCATAGAAGTTGCTCAAATCGGGAACAAGATCCTTTAT10.8928571428571428No Hit
ACCTAGGCTCTTGGACGAGATGGGAAGGCACAGGGAGAAGGGATAACCCT10.8928571428571428No Hit
TCACCACACAGTCTGCTGAGGTTGGAGCTGCTGAGACGACGCTCACAGAG10.8928571428571428No Hit
GGTCGGGGGGGGGGGGGGGGGCGTGGGGGCGTTGGGGGGGAGGGGTGGGT10.8928571428571428No Hit
GTGCATAAGGACGAACTCTACAGCAGCCCAATCTTCAGGCTAGTGGGATT10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTCGGG10.8928571428571428No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.8928571428571428Clontech Universal Primer Mix Long (100% over 23bp)
GTGTACATGGGCAGCAGTGGGATCAAAAAAAAAAAAAAAAAAATAAAAAG10.8928571428571428No Hit
CTTCTCATCACAGCTGTCCTGTGTGGACTTGAACTACTTTTAAAATGAAA10.8928571428571428No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAGAGAACAAGGGAAGC10.8928571428571428Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGGGAACAGCTGCGGGCCGGGCAATACCGCACCGCCATCTTCAAGCGCC10.8928571428571428No Hit
CACATGTACACAGCTTTCATACCACTGCTACACATGAACAAACATATTAT10.8928571428571428No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8928571428571428Clontech SMART CDS Primer II A (95% over 23bp)
TGACAGTGCTGCCAGGGGCAGAGCAGAGAGTGTCCAGGGAGCCAGCCGGT10.8928571428571428No Hit
GGCTTGCAGCCTGGCCTTCTCTATTTCTGCAAGTTCTTTTATGGAATGTC10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG10.8928571428571428No Hit
TCCAAGCAGAGTGCGTGGGAAGCTGGGGGAGCGTGGCTGAGTACAGGGGA10.8928571428571428No Hit
GTGGATTTTCTTACCTACTGCTTAGACGAGGAACTAGTGTGGGCCTATGC10.8928571428571428No Hit
GGGTCGATGATGCTGATGCTGACAGCCCGGCTTTCCTGGGGCCCGTGGGT10.8928571428571428No Hit
ACATTGCTTAATATTAATTTTGCATTAAAATTTGGCCTGATTGATGATAG10.8928571428571428No Hit
GCTTCCAGGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
ACCTAAACGTCCCCATTCAAGCCTGGCGTCCTGTTGCCTTCCCGCGGGGG10.8928571428571428No Hit
GGGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.8928571428571428Clontech SMART CDS Primer II A (96% over 26bp)
CCGTCTCCTTGTCCCTGCTTCATGTTTGGGGGCCTTTCTTTAACTGCCTT10.8928571428571428No Hit
GGCCAATTTTTTCCTCGGAGCTCAGGCTGCGGGCACTACTCATATACTCA10.8928571428571428No Hit
GCGGTTAACCAATTTCAAAGAGATAAAGAGCACACACAGCTAGAAACAAA10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGTGGGGGGG10.8928571428571428No Hit
GGGGGGGGCGGGGGGGGCGTGGGTGGGTGGGGGGGGGGCTGGGGGGGGCG10.8928571428571428No Hit
GGGGTGGGGTGGTGGGGTGGTGGGTTTGGGGGGGGGGGGGGTGGGGGGGG10.8928571428571428No Hit
TCACCACACAGGCTGCTGAGGTTGGAGCTGCTGAGACGACGCTCACAGAG10.8928571428571428No Hit
TATGTAGGCAGAGGACTGAAGGTGAAAGGGAAACTACTCAGTCTTGGAGA10.8928571428571428No Hit
CCCATGTCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTAGAGGTCTTGCAGATCAAGAGGAAGCAGCAAAGGAGCCTGGAAATGA10.8928571428571428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGTGTGGGTGGCGGTGGCCTGCGTGGACTTCGGCGCGTGCGCCGTA10.8928571428571428No Hit
GGGGGGGGGGTGGCGGGGGGAGGGGGCCGTGGGGGTGGGGGGGTGGGGCG10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGTG10.8928571428571428No Hit
CCGTCTATTCTATTACAACTACTCTCATTTCCCTCTTTTCTTTTCAATCT10.8928571428571428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGG10.8928571428571428No Hit
ACGCAGAGTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTAAAATAGGCTGGAGAACAAGCTTGTCCAACCTGAGGCCCAGGACAG10.8928571428571428No Hit
ACAAGCATATGAGTCAGCAATTGAATTTTTAAGAAAAAGAAAATAAGTTT10.8928571428571428No Hit
CTACTCATCACAGCTGTCCTGTGTGGACTTGAACTACTTTTAAAATCAAA10.8928571428571428No Hit
GGGGGGGGTCGCTCGCGCTGGCCTGCGACGTCTTCGGCGCGTTCGCCGGT10.8928571428571428No Hit
GTACAACTGCTTCCCAATTACTCTGCTTTGATACCAATGATTCCCAGTAC10.8928571428571428No Hit
GGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTC10.8928571428571428No Hit
GTATCAACGCAGAGTACTTATTTTTTTTTTTTATTTTTTTTTTTTTTTTC10.8928571428571428No Hit
GTGGAGGGACGTTCAGAAAGGGTGCATTCTGGCTGCCGGAGCAGAGGGCT10.8928571428571428No Hit
CCCCCACTTTAGGCAATGTGTGTAAACTGCAATTGACATTGTGGAGTGCC10.8928571428571428No Hit
GCATATATTCTTTATAACCCCACCCTAGAAAAATATGACATCACATACTT10.8928571428571428No Hit
GCAAAGAGAACATCTGTCTTATTGGCAATGCCTTTCCTACTTATGATTTT10.8928571428571428No Hit
GGGGGGGGGGGGGGGGGGGGGGGTCGGGGTGGGGGGGGGGGGGGGGGGGG10.8928571428571428No Hit
TCCCTGCTGTATGACATGCATTTGGGAAGTTGGAAAGAAATGACAAAATC10.8928571428571428No Hit
GTCCCTCAACTTCTGAAAAAAATTATTTGGTCATACCAAACTCTTGTCAT10.8928571428571428No Hit
GGGTCGTTGATGCGGATGCTGACAGCACGGCTTTCCTGGGGCCCGTGGCT10.8928571428571428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)