FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125.47945205479452Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC73.1963470319634704Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52.28310502283105Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52.28310502283105Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC52.28310502283105Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA41.82648401826484Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG31.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.36986301369863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.36986301369863No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG31.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31.36986301369863Clontech Universal Primer Mix Long (100% over 23bp)
CAACAGAAAGCTTTTCATAATTGTGAGTTAGGATCATGTCAATTTAAGTA31.36986301369863No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGATTATATCATGTGCACAACCAACAACATAAAACCTTTAAAAATACAGA31.36986301369863No Hit
CGTCTCCTCGGCTAGATCAATAAAACTAGCCGACAACTTTTAAGACTTTT20.91324200913242No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20.91324200913242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT20.91324200913242Clontech SMART CDS Primer II A (96% over 25bp)
GTATTATTGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTTTCTTT20.91324200913242No Hit
ACATTAGACAGATCAACGAGACAGAAAGTCAACAAGGATACCCAGGAATT20.91324200913242No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.91324200913242No Hit
CAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAA20.91324200913242Clontech Universal Primer Mix Long (95% over 22bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GTACACGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGT20.91324200913242Clontech Universal Primer Mix Long (95% over 22bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.91324200913242Clontech Universal Primer Mix Long (95% over 21bp)
ACCATACTCCCCCCGGAACCCAAAGACTTTGGGTTCCGGGGGGAGTATGG20.91324200913242No Hit
TCCCCAAACTAGAGCTTTCAAATCTTTGCTTTTAAAACTGGAAATCACAC20.91324200913242No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GACCTGACCACCGGTTCGCCCAATGTGGTGGTCGCCGTGATCGACACCGG20.91324200913242No Hit
ATTAAGAATGAAGCTGATAGGACCTTGATATATATAACTCTCTACATTTC20.91324200913242No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGTACATGGGAAG20.91324200913242Clontech SMART CDS Primer II A (95% over 23bp)
AAAGATTACGCTGGCGGTACAGATCTGGGCGTTTACGGTGCGCACGACAA20.91324200913242No Hit
GTAAATTGGAGTCCGACAAGCCTGCAGTTCCGTCGCCAACCTGATAACTC20.91324200913242No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCCTACATTTGCTATGCGTCTCAAGATAAGGATATTATCTCATATGG20.91324200913242No Hit
ATGTAAATTTATAAATATATTCAGGAGTTGGAATGTTGTAGTTACCTACT20.91324200913242No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GATTGCCTGCTGGATAAAAATCAAACCAGCCACCTCGCGTCTGCGCAGTG10.45662100456621No Hit
GAGTACATGGGAAGCATTGGTATCAACGCAAAAAAAAAAAAAAAAATAAA10.45662100456621No Hit
TATCAACGCAGAGTAATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.45662100456621No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCC10.45662100456621Clontech SMART CDS Primer II A (100% over 26bp)
CTCACATAGTTTAACCTTTCTTTTCATAGAGCAGTTTGGAAACACTCTGT10.45662100456621No Hit
GTACATGGGAAGCAGTGGTGTCAAGGCAGGGTACTTTGGTGTTTTTTTTT10.45662100456621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTA10.45662100456621No Hit
GTTCACAACTCTTCGATTTGAATTGTAATGAATCTGGTGACAAGGATTTT10.45662100456621No Hit
GATCAAGGGAATGTTCAAGCGAAAAGCCGAGCAGGACGGACCTTCCAAGA10.45662100456621No Hit
TAACTGAAGTTCTTGGGATCCAGTCCTGCAGCGTGTTTTGCCTACAGACT10.45662100456621No Hit
GATTGCCTGCTGGATAAAAATCAAACCTGCCACCTCGCGTCTGCGCAGTG10.45662100456621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTG10.45662100456621No Hit
GTGCTGTTGGGTCCTTCAGCTCCTCGGGTGGTAGGGAGGACACAGGCTGG10.45662100456621No Hit
GTCTTGGCTCCTAGCTCAATGTTTTTTCACTAAAAACTGACTCGTAACAC10.45662100456621No Hit
GTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTGGAACTTCTAGTAGAGGACGAGTCCGAAAGTTGACTGAAAAAGCA10.45662100456621No Hit
AAATCATTGTCCTTTCCCTTAAACATAACACACACTAACTTTGAAAGGGA10.45662100456621No Hit
GTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGT10.45662100456621No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGG10.45662100456621Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACAAAACAAATAATTCAGTAGGAAGAAAATCTCATGGGATCAAGCATT10.45662100456621No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAACAAAG10.45662100456621No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACCTGTCTCTTATACACAT10.45662100456621Illumina Single End PCR Primer 1 (100% over 24bp)
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT10.45662100456621Clontech Universal Primer Mix Long (96% over 27bp)
ACCATCTGCTTCTGCTGAGGCCAACATCCAGTGCCGATGAGCGACCAGCT10.45662100456621No Hit
CTGGAGACAAAAGCAACAGCTCAGAAGTGCTAAATCTTGAAGAGCAGCCA10.45662100456621No Hit
GATTAGTTACACCAAGCAAGAGAAGATATAATGTCTCGCTTTCACATTTG10.45662100456621No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGATTTCTATTAAACACATCT10.45662100456621Clontech Universal Primer Mix Long (95% over 23bp)
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA10.45662100456621No Hit
GATGTCCGTTCATATGATCTGTTTGCTTTGTTAGTCGAGAAAACTACCTT10.45662100456621No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT10.45662100456621Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCAGTGCTACACATGAAATCTCCGAT10.45662100456621Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTCAGAGAACAAACTATTTGATTTCAACCTTTTTAATATTTATTAAAAC10.45662100456621No Hit
GTACATGGGAGACTGAGGTGGAAGGATCATTTAAGGCCAGGAGTTCAAGA10.45662100456621No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTC10.45662100456621Clontech SMART CDS Primer II A (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCGGCTT10.45662100456621Clontech SMART CDS Primer II A (96% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTTATA10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
TCCTGAGTAGCTGGGATTACAGGCGCGTGCCACCACGCTCAGCTCATTTT10.45662100456621No Hit
GAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG10.45662100456621No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC10.45662100456621Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAAGACCGTGTTCCTGTGGGTCTGGTTTCTGAGGGTCAAGGCGGAGG10.45662100456621No Hit
ACTCTGCGTTGATACCACTGCTTACCCATGTACGCTGCGTTGATACCACT10.45662100456621Clontech Universal Primer Mix Long (96% over 27bp)
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT10.45662100456621No Hit
GACCACTAGCAAGACTAATAAAGAAAAGAGAGAAGAATCAAATATACACA10.45662100456621No Hit
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATTTAAACAAAGT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTGGGCAGAGGGACTGGGAAATTCAAGGGATGGAGGAAGAGGAGAGG10.45662100456621No Hit
CTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTCAAGCTCCGCC10.45662100456621No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGGGCATTCCTGAAGCTGACAGCATTCGG10.45662100456621No Hit
ATAGTCGCTAGAACACTCATGACAATTTATTTCTGGGAAGAAGCCAGGAT10.45662100456621No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45662100456621No Hit
GTCACTGACTTGGGTATCAGTTTCCCCTTCTGAGAAGTACAGAGTGAGAC10.45662100456621No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC10.45662100456621No Hit
CTCATGTTCACACTAGTCATCATTACTGGGGTCTGACTTAGCATTTCATC10.45662100456621No Hit
CCGTTTTTATTTAATATTTTAAGGTAACGGCAACAATGGCGAATTAAAGA10.45662100456621No Hit
CCATGGGAGAGGGCAGCACTCCTGCCTTCTGAGGGGATCTTGGCCTCACG10.45662100456621No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
TTGTTAGCTAGTTGGCATCAGAAAGTCTAAATTTATGCCCTCTAACAACC10.45662100456621No Hit
ATCAAGACAAACACAAAGAGTACCAGAAGTTGGCATTAGAGCCGGATATT10.45662100456621No Hit
CAGCCGCTGGGTGCGTGTTGCGCAGCGGCAGGCCGGTTCCGGTATGGGCT10.45662100456621No Hit
CATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45662100456621No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA10.45662100456621Clontech Universal Primer Mix Long (96% over 27bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTATATAAAAA10.45662100456621No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGT10.45662100456621Clontech Universal Primer Mix Long (95% over 21bp)
CAGCCGCAACGCCATCCGCATGGCCGCGTTGATGAAGCAGCGCGTCATCC10.45662100456621No Hit
GTACACAGTGGGCTCCTCCCCCTCCTTCAGCAGTTTGCCCACGTGATGAT10.45662100456621No Hit
CCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTACTCTGCGTTGAT10.45662100456621Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTTATGGGGAGACTGTGTCAGTCTAATTATTGTTCATCTTCTGTTTTT10.45662100456621No Hit
GTACTCTGCGTTGATACCACTGCATCCCATGTACTCTGCGTTGATACCAC10.45662100456621Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTAGTTTCAGAATTAACCCCCATTCAAGGTCATCCACTTCAATTTAGTG10.45662100456621No Hit
GAGTCTTGCTCTGTCACCCAGGCTGGAGTTCAATGGTGCAATAATCGTCC10.45662100456621No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTTCGTAGAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GCTGGAGTGCAGTGGCAGGATCTCAGCTCACTGTAAACTCTTGCTTCCCA10.45662100456621No Hit
CCTTACACCTTATACAAAAATCAATTCAAGATGGATTAAAGACTTAAACT10.45662100456621No Hit
CCATTCGAGGATGATTCCATTCGATTCCATTCGATGATTCCATTCAATTC10.45662100456621No Hit
CCACCTATGAACTCTCTAGGTCATGAGGCTTGCTGCTGTATATACAAGGG10.45662100456621No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
AAAAGGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAAC10.45662100456621Clontech Universal Primer Mix Long (96% over 25bp)
CTCGCTTTGTTACTCAGGCTAGAGTGCACTGGCACAATCAGAGCTCACTA10.45662100456621No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
ACATAGAAGAATGCTCAAACTTTACCTTCTGGTCAGTTCGACCAAACTTT10.45662100456621No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAT10.45662100456621No Hit
CTATCGTTGAGACGGCCATGAGCTCAATAAACTCACTATATCTTTTGTCA10.45662100456621No Hit
GCAGAGTACATGGGAAGCAGTGGTTCAACGCAGAGTACATGGGAAGCAGT10.45662100456621No Hit
TCCTTATGTCTAGTAATCAGTAATTTTTTTTCTTTGTTCTTCTTTTTTTT10.45662100456621No Hit
GTTTTTGATAGTCACGTGATTGACTGTCAGAGCCTGGTCCATCTCAGAGA10.45662100456621No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45662100456621No Hit
ATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTC10.45662100456621No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45662100456621No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCCACGTAGCTGGGATTACAGGCATGGACCACCATGCCTGGCTAAGTTTT10.45662100456621No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCT10.45662100456621No Hit

[FAIL]Adapter Content

Adapter graph