FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA156.8493150684931505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT83.65296803652968Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73.1963470319634704Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG52.28310502283105Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41.82648401826484Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41.82648401826484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAAATGAGGGTTTTGGCACCACTAATACAGTTAAATTAGAATAGGTA31.36986301369863No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA31.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTTTGCTACCTTCTGAAATTTGAGAAATAGTAAAATTGGAGCACCTTCAG31.36986301369863No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31.36986301369863Clontech Universal Primer Mix Long (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA31.36986301369863Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GTGTACATCTTGCTGGAAAATGCTGCCCAGGGCTCTGGAGACGGTGGCTG20.91324200913242No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20.91324200913242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGTGGTTTCAATCTCTCCCTTTGGATTTTATAACACCTTAATCTTAAG20.91324200913242No Hit
GCCAACGGGCGAACGTTATTGTGTTAACTCTGCCTCTTTACGCTTTACCG20.91324200913242No Hit
CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG20.91324200913242No Hit
AATGGAGGGTCTGCTCCTTGGCCTTACGGTAGAGGCGAGAGCGCTGACCG20.91324200913242No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
TATTAAATATAGCACGAACTGCTTGGCTTTGCTTAACCTTAGTTGTATTA20.91324200913242No Hit
GTCACTTTGTTTAAGCAAACACAAGTACAAAGTAAAATAGAACCACAAAA20.91324200913242No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.91324200913242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA20.91324200913242No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.91324200913242Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT20.91324200913242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.91324200913242No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC20.91324200913242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.91324200913242Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATTTACGGTTGTAACGCTCGGAGTGCGTTTTGTCGGTAAATACGCCGGTC20.91324200913242No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.91324200913242Clontech Universal Primer Mix Long (96% over 26bp)
CCCGAGACGCGCGTGCCATGCCAGGAGCTGTTGCTGATGTCGGCCGAGGT20.91324200913242No Hit
GCCGGGGGCTGGAGGACAGGTTTGTGCGCTGGACGCAAGCACCAGGCGCA10.45662100456621No Hit
GTGTGTTGCTTAGTTTCCAAATGCTTGGGGTTTTCCTAGTTATCTTATTG10.45662100456621No Hit
AAAAAGTACTCTGCGTTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.45662100456621No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG10.45662100456621No Hit
GTAATCCCAGCAGTTTGGGAGGCCGAGGCGGTTGGATCCCAAGGTCAGGA10.45662100456621No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATATTCAGAAATATTTGCAAATGCATTCATAATTAGATCAATCAGATA10.45662100456621No Hit
ACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACT10.45662100456621Clontech Universal Primer Mix Long (96% over 27bp)
AATTAAAACATTGTTAGGCATTTTAAGGACTTGTTCTAGAGATCAGTGAA10.45662100456621No Hit
CTACAGACACAGGCCACCATGCCCGGCTAATTTTTTTTTTTTTTTGTAGA10.45662100456621No Hit
TAACATTCCCTACTCCACCACAGCAGCAAGGAAGCAGAAGCCTTAGTTCT10.45662100456621No Hit
ATATATGTGCATATGAGTATATGTCTATATATACAGACTTGCATATTGAT10.45662100456621No Hit
GACTGAAGTACAAAAATGAATCCCAAGTAGGATAAATATGAATAAATTAC10.45662100456621No Hit
AACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACAGCTTCTTCGGCCTAGTGAGGCGGGGGGGTGGTTGGAGGCCCCCCGG10.45662100456621No Hit
GCTTCCCTCAAGCCCGAATTCGTGGACATCATCAATGCCAAACAATGAGC10.45662100456621No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
ATGATGGTGTGGAAAACTAACCAGCAGCACTCCTGAAATGCTGCCTTCGT10.45662100456621No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACT10.45662100456621No Hit
TCCTAATACAACACTTCTTTCCTAGATACCTGAAAGGAAACCTTAAAATT10.45662100456621No Hit
TAGTAAGAAAGGATACAGGGGTGAGGAAGCCTATTCTCTCAATGACCAGA10.45662100456621No Hit
CCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG10.45662100456621No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.45662100456621Clontech Universal Primer Mix Long (95% over 22bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGGGTTAGTTTC10.45662100456621No Hit
GCTGTATGAACGCCACGTTTGTGAAGAGCTGAGGGTATCAGGAGGGCCGA10.45662100456621No Hit
CCTAAAACATTTTGGCAACCTGACCAAAGTCACACAGCAAGTTGCTGAGA10.45662100456621No Hit
GTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGG10.45662100456621Clontech Universal Primer Mix Long (96% over 27bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGTTTTAACCAATGCTA10.45662100456621No Hit
CAGACATTCCCACTGGCCCTTGCAGCCACATGGTTCTCCATCCCTCTTGC10.45662100456621No Hit
GTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGG10.45662100456621No Hit
GTGGTATCAACGCAGAGTACATGGGAATCAGTGGTATCAACGCATAGTAC10.45662100456621No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTTATA10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC10.45662100456621Clontech SMART CDS Primer II A (100% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTGTACACCAAGCACCTATTTTTATAACTTAGCTTCCCATGGAGAGA10.45662100456621No Hit
GGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTAAACACATCTGACGC10.45662100456621No Hit
GGGGATAGGAATTCTGGTGCTCCTGATCATCGTGATTCTGGGGGTGCCCT10.45662100456621No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGTGAAACCCCATCTCTACTAAAAAATACAAAACTTAGCCAGGCAT10.45662100456621No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GGTCCTGGACGCCGTCGAAGCGCCGCAGCTCGGCGGGGGTGAAGTCGCGC10.45662100456621No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45662100456621No Hit
AGACAAGTCTGAATGCTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTA10.45662100456621No Hit
GAGAAAATTTTGCTGTGCAGAAGCTCTTTAGTTTAATTAGATCCCATTTG10.45662100456621No Hit
GTTGATACCACTGCTTCCCATGTACTCTGGGTTGATACCACTGCTTCCCA10.45662100456621Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCTAAGCACAGCCCCCTCACTGGGCAATAAGTCAGCAACAGCAATGCTGG10.45662100456621No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGTTGCCTTGCGCACTCGCGGCGCTGTCGCTGCCTTGCTTGTAGAGGGC10.45662100456621No Hit
TGCCTGTAATCCCAGCTATTTGGAAGGCTGAGGCAGGAGAATTGCTTGAA10.45662100456621No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAACTGTGAGAGTATTATTTGTTAAAAAGTTGGACTTACTTGCATTTTG10.45662100456621No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAACAGTGGTATA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGTTA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GGGACGTTCCACTCTGTGAGTTGAATACACACAGCACAAAGAAGTTAGTG10.45662100456621No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTCTGTTCACAAATTCTTCATTCAGTCCTGTCTAATCACACTTCAG10.45662100456621No Hit
GGCTCGGGCGCTGGGCATTGCCCAGGGCCATGGTCCAGGCCACCACCGTC10.45662100456621No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAACCACTGCT10.45662100456621Clontech Universal Primer Mix Long (96% over 25bp)
GCTCCCATGTATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.45662100456621Clontech Universal Primer Mix Long (95% over 24bp)
TTTATATAGGAAATGAAGACCGAAAACTCTAGCTCCTGACTGCTCTTAAA10.45662100456621No Hit
TATCAACGCAGAGTACATGGGAAGCATTGGTATCAACGCATAGTACAAGG10.45662100456621No Hit
ACAAATATGTTTTCAGTTGAATTGGTTGTTAGAGGGCATAAATTTAGACT10.45662100456621No Hit
ATGTTAGGGTGTCAATTTTGGATCTTTCCTGCTTTCTCTTGTGGGCATTT10.45662100456621No Hit
GCAGTGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCAAGTACTCCTGTCTCTTAAAAACATAT10.45662100456621Clontech Universal Primer Mix Long (95% over 23bp)
AGCTTCAACACAGCAGAAATTTATTTCCCACCCAGGTAAGGGGACCCTGA10.45662100456621No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTGATAACAAAAACAGGAGCAAAATACAAGATAAGGCTTGGCAGGGTAG10.45662100456621No Hit
GTAAATGGATTTCTAAAATGCCTGAAGGTACTTGAGAAGAAAATATTTTA10.45662100456621No Hit
ATGCACAACTATGCTCAGGTAATTTTTTTCACTTTTTGTGGAGATGGGGT10.45662100456621No Hit
ATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAAAAAAAAACGAAAAAA10.45662100456621No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.45662100456621No Hit
CGTTGTGTCTTTGTTCTCATTCGTTTCAAAGAACATCTTTATTTCTGTCT10.45662100456621No Hit
GGTGTATGTACGCTGCCGTGTGACCCATTTTACGGATAGGGGAGCGGCGG10.45662100456621No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT10.45662100456621No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTC10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCTCCAGAAGGTTGCATTCTAGAGTCAGGTATGGAATTTGGGACTGCTTT10.45662100456621No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.45662100456621Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
AATGAGAAGATCACATGAAGTCAACTATTGCTACAATTCTCCTAAAATGT10.45662100456621No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCT10.45662100456621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.45662100456621No Hit
CTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTC10.45662100456621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAC10.45662100456621No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTAT10.45662100456621No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA10.45662100456621No Hit
GAATGGAATCATAGAACGGAATCCAATGTAATCATCATTGAATTGAACCC10.45662100456621No Hit
ATGTTAGGGTGTCAATTTTGGATCTTTCCTGCTTTCTCTTTTGGGCATTT10.45662100456621No Hit
TGGTGGCGTGCATCTGTAGTCCTAGCTACTTGGGAGGCTGAGATGGAAGG10.45662100456621No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGTTATCAAC10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCCGGGGTATAAAACAAGTCT10.45662100456621Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTCCCAAGGGGACTTAAGCGCGGTGACCTCCCCTATCCCCTACGAG10.45662100456621No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACT10.45662100456621Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAATGATACGGCGACCACCGAGATCCTGTCTCTTATACACAT10.45662100456621Illumina Single End PCR Primer 1 (95% over 24bp)
TTATAGCTCCCTGTAGACTCAAAATCATGGGCTCCAGGGATCCTCCCACT10.45662100456621No Hit
NGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTC10.45662100456621No Hit
GTATTAGGGAAATAGACCACGTTATCTTTCGCCAGCCGCGTGTTAGTTTC10.45662100456621No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG10.45662100456621Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph