FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length140
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCATGACGAGGTTGCCGCCCATGATGCGGCGGAAGGCGCTGCCCCAGG32.6548672566371683No Hit
GCTATCATGTGCACTCCGTACCCCAGCCATCTTGCATGTGTATGCTCTTC21.7699115044247788No Hit
CCCCCTGTCGCCGGTGGAAGGTCAACGCCATGGGCGCGCCCGAACTGCTC21.7699115044247788No Hit
GTGTAGCCCAGGATGCCCTTGAGGGGGCCCTCCGACGCCTGCTTCACCAC21.7699115044247788No Hit
CACCGCGCCTGCCGCCTCGGCGCCCGGGACGTCGCCGATCCAGAGGTCCT21.7699115044247788No Hit
GTGTCCATGAAGGTGAGCGGACGAGCGCGGACCGCGAGCGGCACCGCCCG21.7699115044247788No Hit
GACCTTGATCGAGGAGATGTCGGTGCGGGCGCCGCGGTCACCCCGGGCGA21.7699115044247788No Hit
GTAGATGACGTCGCGGGCCGCGACGAGGTCGCCGGCGGCGCGCCAGGCGG21.7699115044247788No Hit
GCGTGACGCTGCGCCCGTCGGCGAGCAGCCCGTCGATGACCTCGCGGACG21.7699115044247788No Hit
TCGTGCAGCTGGCCGTCGCCGTCGTGATCGGCACCGCGTTCGCGGCCGTC21.7699115044247788No Hit
GTGGTCGTACAGCGTGGTGAACACCGCGCTCACGAAGGCGTAGGCGGCCT21.7699115044247788No Hit
CAGTGAACCAGATTTCTCCAGTGCCTGCCGAGGGTTTCGCCCCGGCCGAC21.7699115044247788No Hit
CTCCGGGCACCTGCGTCCTACCAGCTCCTGACCTGGCTGGAGAGCGGGCC21.7699115044247788No Hit
GTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGG21.7699115044247788No Hit
GCGCAGAGCAGCAGGCCGACCGCGACGACTCCGGCGAGGTGCCGCTCTCC21.7699115044247788No Hit
TTCGGCACCCTGGCGGGCCAGCTCCATAGCCTCGCGCATAAAGCGCTCGT21.7699115044247788No Hit
GCTGTGGAACCAGGGGTTGCGGACCGGACCCCCGGCCGCGCACGACCAGC21.7699115044247788No Hit
GGAGCACCGCCACGTTCGCCAACGTCGACCTGAGCGTGGCCCTGCACCGC21.7699115044247788No Hit
GACCGGTACTGATCCGGGTCAATCTCGGCGGGAACGCCAGGGGGGTATGA10.8849557522123894No Hit
CCGTAGTCGTGCGACCAGAAGCGCATGCCCCGTTCCGCACATGTGTCCGA10.8849557522123894No Hit
GGTGAGCACCGCCCGGCCCGGCACGACGATGCAGACCGAGTCGGCGGTGT10.8849557522123894No Hit
ATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAG10.8849557522123894No Hit
GCTCGGTATCGCGAAGGCGTGAGGCCGGTGCAATCACGGAACGCGGAGCT10.8849557522123894No Hit
CCTCGTCGGTGTCGGCCGAGAACGAGGCGGGGTCGGATGCCGGGAAGGTG10.8849557522123894No Hit
TCGCTGGAGCCCCGCCGCGGCCTGGTCCTCACCGCGGTGGCCGTCGTGCT10.8849557522123894No Hit
GTGGATGGGCGGCGGCACGAGCGCGAGCCGCGTGCGGTGCCGGATCGTGT10.8849557522123894No Hit
CACGCAGCGCTCCTTCCTCGCCTACGACGCCCGCACGCCGACGCCATAGC10.8849557522123894No Hit
CTCGTGGTCCGTCGGCAGCCGCGCACCGACCGCGGCGGCGAAGCGGCGGG10.8849557522123894No Hit
ATCCAACGCCTCGCAGGCGTGGCTGGCAGGAGGGGCCCGGCCGTGCCCAG10.8849557522123894No Hit
GTAGTGGCCGTGTTCATTCGCATGCCGATCATGGGCTACCGCGATAGCGT10.8849557522123894No Hit
GGTCGTAGTCGGTCGGTGTCGCGACCACGACGAGCCCGGCGCCCTCGAAG10.8849557522123894No Hit
GTTCCTCGGCATCGTGGCCGGGCTGCCGAGCCCGGCGCCGCAGCTGCACT10.8849557522123894No Hit
GAGTGATCACGACGCCCACCCTGGTGATCCACGCCGAGGAGGACCGGCGC10.8849557522123894No Hit
CTCGGCGTGCCGCGGGCGGTCGAGCACGGCGACGACGATCTCCTCGACGT10.8849557522123894No Hit
CCTGGAGCTCCGCGTCCCGCTGGTCGGTGACGTCGTAGGCCGCGCCCAGC10.8849557522123894No Hit
CCCCAGCCGTCCTCGGCGGCCGGGTGGGGCCACAGGCCGCCGCGGAAATT10.8849557522123894No Hit
CCCGGCGGGCGTCGGTGTAGTTCTCCAGCAGCGGCTTGAGCTGGACCCCC10.8849557522123894No Hit
GCTTCTGGGACCTGCCAGCACCGTTTTTGTGGTTAGCTCCTTCTTGCCAA10.8849557522123894No Hit
ATGGAATACTGTCGCGCTGGGCAACTGCGGCATCGGCAAATTCAGCGACA10.8849557522123894No Hit
GATCTCGCCGCGCGTCGTCGACAGGTCGATGAGCGCATCGCCGTCGACCG10.8849557522123894No Hit
CATCGAGATCGAGTCGATGTCGAGGTCGTCGGTGAACGACTTCTCCATCT10.8849557522123894No Hit
TGTATACCTCGCGTGCGCGGCCCAGCTCGTCGAGCACTTCAACCTCGTCG10.8849557522123894No Hit
ACCCACGGCCCATCCGCGCGCAGCGCCGCATCCGGCTCGAACGTGCACTC10.8849557522123894No Hit
GCCTCGGCCTGGGCCTCGGCGGAGAGCGGCACGTGGACCGCCATCTGATC10.8849557522123894No Hit
ATGCCGCACAGGTGCGACGCCGAACCGACGCCCGCGTTGGCATAGGTAAC10.8849557522123894No Hit
GCCAGGCGGCCCCGGCGAGGATTCGGTGTCGCCATCGTGGAAAGGACGAG10.8849557522123894No Hit
AGCCGGCACCGGCCCCCGAGCCCGAGATGGGCGAGCCCGTCATCGAGCGA10.8849557522123894No Hit
GTCGGGTACACCGACGAGGCGACGCCGGTGCCGACCAGCCAGGACCCCTC10.8849557522123894No Hit
ATCCTGCGCTACATGAACCATGTCGCCGACCGGTACGACCTGCGCCGGGA10.8849557522123894No Hit
TCACGGACATTATGTGGCCAATTATCGCAAAGCTGGCCCGAGAGGAGATG10.8849557522123894No Hit
TCGTCCCCCTGCGGCGGCCCGCCGGCCCGCGATCTACCTCGGTGTCGCCG10.8849557522123894No Hit
TGCGATAGCAGCAAGCAGGCCAGCCATCGCGCCCGATAGCGGTAACGGCA10.8849557522123894No Hit
GACGCGCACCATCCGCCGATCGGTGAACGAGAAGCGGCGCGCGGGCACGC10.8849557522123894No Hit
CCCGCATGTCGAACGCCGTCACCTTGCCGCCGATGAGGCGGTTGGTGCCG10.8849557522123894No Hit
CGCCGTCTGGCTCGACGAGCGTCGTGAGGTCGTCCTGCTGCTCGGCCGGG10.8849557522123894No Hit
ATTCCTGCGGCGTGTGCGATGCCGCCAGCGTGGCGCGCCACTCCAGCAGC10.8849557522123894No Hit
GCGTCGTGATGACGCGGGTCGCGGCCTGCGTGGGCGAGTCGATCGTGGCG10.8849557522123894No Hit
AGTAAAAGTAGAGCTGGAGGGGGTAGATGAGCACAGAGCGTGCAGAGTGC10.8849557522123894No Hit
TTATTACTCAAAAAAGTTTCATTTTTTTATTTAGCTTTCTGACTCTGTGC10.8849557522123894No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ACCTGATTCCGTTCAATCCTTTCCCCGAATCGGGCCTGCTGCGCTCGAAA10.8849557522123894No Hit
GGATCAACCTTTCCCGGCAAGGCGGAGGATTTGGGGCAAGAGGTCCCGGG10.8849557522123894No Hit
GTCCTGGACCTTGACGATCTTCTCCCCGTAGACCTCCCCGAGGCTGCCGC10.8849557522123894No Hit
GCTTCAACGTGCGCCCCGACCCGAAGGCACGAGAGCGCGCCGCCCGCGAG10.8849557522123894No Hit
GTGCGGGTCAGGCGCGGCCGGGCCGGACGACGCGCTTCACGGCGCTCGCG10.8849557522123894No Hit
CACCGTGGGCCAGGCTCATGAAGAGCTCGCCGGCGATCTCGGCATTCCAG10.8849557522123894No Hit
AGCCCGGGACGAGCGCGAGCAGCAGCAGCGGCAGGAACACCGCGAGGTTC10.8849557522123894No Hit
CTGCTGCGCCAGCCGCATCGGCGCTGGCGTCGCCCATGCTGCGGTTTTGG10.8849557522123894No Hit
TGTGACAAGAACATGCCGGATTTCAGCACCCATGACGGTGCCATTTCCTT10.8849557522123894No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT10.8849557522123894No Hit
CTTGTAGAGCGACGGCGGCTTCACGCCCGCGCGCGCGGCGACCGCGCCGA10.8849557522123894No Hit
GTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATA10.8849557522123894No Hit
ACGTCGTGCTGATGCGCTCCGACCCGCTCGACGTCCCGATCGCGCTGACG10.8849557522123894No Hit
GGGTCGCCCCGACGCCGACGTGCGGTCCGGTCACGCAGGGGATCGACACC10.8849557522123894No Hit
GGCAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAAAATGT10.8849557522123894No Hit
GTCGAGAAGGGCTCGGAGATCCTGCGCGCGATCACCTCCGACGAGCGCGC10.8849557522123894No Hit
GGCCGGTGGCACACCAGCGACAGACGCCGCAGTTGGGGATGAAGGAGCAG10.8849557522123894No Hit
GGCGGTAGGTGATGCCGGCGGCGTCTGCGGCGATCGCGCCGCGGTAGCCG10.8849557522123894No Hit
CACGAGAGCTGCACCCGGCTGTCGGAGGTGTTGACGTGCTGCCCACCGGG10.8849557522123894No Hit
GGCCTTCGGCGGCCTGGTGGTCCTCGTGCTGCTCGGGGCCGCCGTCGCCC10.8849557522123894No Hit
GTCAACGTGCTGCGCGCCTACGGCGCCGAGGTCGTCGTCTGCCCCACCGC10.8849557522123894No Hit
GGTTGACGTACGATTCGCGCACCACCGCGCCTTCTTCGGTCCACGCGATC10.8849557522123894No Hit
AACCGCCCCAGCCCTGGCGGGCACGAGAGGAAACTGGTGACCAAGCTGCA10.8849557522123894No Hit
GGTCATCTCGCTGCCTCGCGATTCCTGGGTGTCCATCCCCGGCACAGGCA10.8849557522123894No Hit
GCACGAGACCCAGCGCCGTTCGCGCGGGCGCAGCGGCACCTTCTCGGTGT10.8849557522123894No Hit
TCACCGCGATCTGCGACCGCCGCGCGCTGGGCCTCGAGGTCGTCGAGACC10.8849557522123894No Hit
CTCCTCGGTTAAGTACGCTGCAAGCAGACCATCGGCCGCCGCCTTGGCGG10.8849557522123894No Hit
TTCGCCGGCAGCTCGGGCACGAAGTCGGGCTGCATGTGGGTCGCCACCCT10.8849557522123894No Hit
GCAGCCGGGAGTTGGCGCTGAAGTACTGCAGCGCTTCCTCCTGCTTCTCA10.8849557522123894No Hit
GAATAAATACAACAGGAAATGCAAAAGCTGTAAAGCTAAGGGCATGCAAG10.8849557522123894No Hit
CGCCGGACCCGACGATCGGCAGCGGCGGCGACATGTGCTTCTCGACGCGG10.8849557522123894No Hit
AGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATAT10.8849557522123894No Hit
GCACCACCACCGCGCCCGCGCGCACAGCCACGTCCCGCGTCGCGGCCGCC10.8849557522123894No Hit
TACTTATTATTATCATCCAGTCGAGCGACAATTGATCTTACTTTCATCTG10.8849557522123894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)