FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length140
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCGTGCCGGCGGATACCAGCCGCGACACGCTCGGCCTCATGATGGCCG32.6548672566371683No Hit
GTATGACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACT21.7699115044247788No Hit
CCATGCAACACACTGCATCCAACGCGCAGACCGGCGTCGACCACGGCGGC21.7699115044247788No Hit
GATCACCGGTGTAGGCGTCGACGAAACCCGGCTCCAGCCGGTCGAAGCGC21.7699115044247788No Hit
CCTCCTCGTCGATGTCGTCGTCCACCCTGGTGTGGAGCGTGCACCGTGGC21.7699115044247788No Hit
GTGCCGCCGCGCACGCGCTCACGGTGCGGTCGGGCATGACGCCGCTCGAG21.7699115044247788No Hit
GGTATTACATCAAAATTTCCCAAGTAATTTAGTGAATATGCACCCTGATA21.7699115044247788No Hit
CCACGAAACCGACGGCGCGGTCGAGGTCGGCCTGGGCCAGCACCCAGGGG21.7699115044247788No Hit
GCCTCCGACCGTGAACGCATCGCATGCACGCAGAAGGGCGGACCCTCGCA21.7699115044247788No Hit
ACCACAGCGAGGGCGGCGCCGACTTCGTCGTGACCGCGCCCTACGGGCGC21.7699115044247788No Hit
ATCTGGGGCCGGCCGACATCGGACCACGTCGAGGTCCGCTACGAGAACGT21.7699115044247788No Hit
ACATGAACTATCGGCGACGCCCGGACGCGATCCCCTGGGGCAGCGACCGG21.7699115044247788No Hit
CGCATCGCCTTCGTCGCCGATAATCCGGGGGTGTGGATGTTCCACTGCCA21.7699115044247788No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT21.7699115044247788No Hit
GACCGACGCCGTCTTCTCGGCACATGATCGCCAGGCGGATGCCGAGCCGC21.7699115044247788No Hit
AGCGGGCGCGCTCGATGGCGATCGACCAGTGCTCCATCGGCTGCTTGACG21.7699115044247788No Hit
ATCTCGATCGCGACCCCCTTCGTCTCGTTCCTCGCCGCGGAGTCGCTGCG21.7699115044247788No Hit
ACCAAGGACGACGTCCTCGTGGTGCTCCTGCCCGACGGCGGCCGCGGCTA10.8849557522123894No Hit
CTACCCGCACGCCGAGCGGCCCGCGGAGACGGCGGCAGCCGTGCTCGCGC10.8849557522123894No Hit
GCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACC10.8849557522123894No Hit
CTAACAAAACTAGGCTGTCCCGAACCCCTGGTAATAGAATTGTTTACCTT10.8849557522123894No Hit
ATCCCGCATCCTGCACTCCGACGGTATCGGGGTAGCGAACTTAGCTGACT10.8849557522123894No Hit
TTCTCGGAGTACATGGAGCTCGACCTCTCGACGGTCGTGCCGTCGATCGC10.8849557522123894No Hit
CGTCCAACCGGCGGACGACGCACCGGCTGGCCGCCCTCGACGTGCCCGTG10.8849557522123894No Hit
GCCTGGGCGGTGGTTTCCTCGTTTTCCATGAATCCGGCAGGCAATGTCCA10.8849557522123894No Hit
CCTCCTGCACGGTCGCGTGCAGGTGGGACGCGGCCTTCGCCACGTCGCGC10.8849557522123894No Hit
GCTCCCGCTTTCGCGGGGTAACCGAAACCCGTCGTCGGCACGACGACGTC10.8849557522123894No Hit
GCTCATGGTAGGCGGCTGCCTCTTGTGCTGCGGCGCCTGGGTCTGCACCG10.8849557522123894No Hit
TTCTCGTACACCGACCTCGAGGTCGACGCGACGGCGCCGACCGACGGCGC10.8849557522123894No Hit
ATCGGAGGTACTGCTGCTGCCGTTGAACCCGGTCGAAGTCGCCACCCTCG10.8849557522123894No Hit
GGAAGACCTCGAGCTCGGCGAGGTCGTCGAGGACCTCCGCGCGCTCCTCA10.8849557522123894No Hit
TCCTGAAGATGTCATCAGAAATGCCTTTGCTTGCTTTGATGAAGAAGCAA10.8849557522123894No Hit
GTTCTCGACCGGCGAGATCCAGGAGCTGCGCGCGCTCGCCGAGCTGCTCG10.8849557522123894No Hit
CAGGTGCGCTACGAGGTGGAGGTGGCGGTCGACGGCGACGACGTCGACGT10.8849557522123894No Hit
GCTCGACGTGCCTGCGGTGGCGGCGCGCTTTGGGCTCGATGCCGACGACC10.8849557522123894No Hit
GTTCGGCACCGCGCTGGAACTGGCCCGGATGGCCGAACGGGCGTTCCGGT10.8849557522123894No Hit
CAGCAGGTCGGCGTGGATGGTCTCTTCGAGCGTGGCGCGGAATGCAGCGA10.8849557522123894No Hit
ATATAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA10.8849557522123894No Hit
CCGCCACGCCCGACTGCTTCTCCTCGAACTCCGGCTTGAGCATGCCCTTC10.8849557522123894No Hit
ACATCGCCACCACCGGCAACACCTCGGCGGCCTCGATCCCGCTCGCGATG10.8849557522123894No Hit
ACCTCACGAAGCAACCCATGCAAGTGCTCAAGGTCTTGTTCGCCCTGCTG10.8849557522123894No Hit
GCTTGTGGGAGTTCCTGCGTGACGCGGCCGACTCCAAGCAGCTGCACACC10.8849557522123894No Hit
GCATCCGCAAGTGGATCGAGCGCCGCGCCTACGCGTTGCCCTATCCGGTC10.8849557522123894No Hit
CTCCGAGATGGCCGAAGAGCACCGCGCCGATCGGCCGGACGACGAAGGCG10.8849557522123894No Hit
GCATACCACCTCTCAGACGCAGGACCAGGTGCAGGGTCGACTCCTTCTGG10.8849557522123894No Hit
GACCCGGCGTGGGCGGATGCCGCGTTCCCCGCCCGCGCGACCGAGCGCAT10.8849557522123894No Hit
GAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAA10.8849557522123894No Hit
GTCGTCGGTCGTGATCACCCAGCGGTTCTGCGACTCGTCGAACACCGTCG10.8849557522123894No Hit
AGAGGCTGCTGTGACCGAGCCCGTGCAACCCGCCGTCGTCGATGACCGCG10.8849557522123894No Hit
AACAACGTCCGCGGCGGCAACACCCAAACCGCGACGAATACCGCGACCCA10.8849557522123894No Hit
CGTCGGCAACGGCCGCGGACCGTCGTGCGACATCGCCGTCGACCCGATCG10.8849557522123894No Hit
GGCATTCTTTGTATCGGGCAGGTGCCCAAGCATGGTGAACGTCAGGTTGG10.8849557522123894No Hit
GTCCAAGCGACTGGAAATCACGGTCTGGCGCAAGGAGCCTGACGGCACCG10.8849557522123894No Hit
ACCGCGGCCTGCGGGCGGCCGGCTTCGGGCGCGAGTGGCAGCTGCCCTGG10.8849557522123894No Hit
ACGCTGGACGGCACGAACACCTACGTGCTGGGCGGTCAGGTCGTCATCGA10.8849557522123894No Hit
CTGCTGGACCCGGGCTCGTTCACCGAGTTCGACGAGTTCGCCCGCCACCG10.8849557522123894No Hit
GCGCCCGAAGTTGGCGCCGAACTTCTGCTGGTACTCGGCCATTTTCTCCA10.8849557522123894No Hit
AGGTGGGGCAGCCGGCCGGCCGAGGTGCGAGCCGATGGAGTCCGCCGACC10.8849557522123894No Hit
GGCTATGTGGCGGTCACCATCGCCGGCGTCGAGCTGCCCTCCTCGCGTCG10.8849557522123894No Hit
GGAGTAGACCGAGCCCTCCGGGTCGGCGCCGACCACCTGCACCCGCCCGC10.8849557522123894No Hit
GATCAACGGCCACCACCGTGGGGCCTCGCGGCTGTCGAGCGCGGCGATCG10.8849557522123894No Hit
ACTTCTTCCACCCCACTTCTTCCTTCACCAACATGCAAGTTCTTTCCTTC10.8849557522123894No Hit
CGTCCAGCTGAAGGATGACTACCTGCTGCTCAAGCAGTCATGGGGTGGCG10.8849557522123894No Hit
CTACCGCGGGATCGCCGCTTCGCTCCCATGCGGGAGGAAGCACGCCAGGC10.8849557522123894No Hit
GCTGGAACGCGCATTTGCCGGTCAGCCGCGCAACTGACCGGCCCCATTTT10.8849557522123894No Hit
GATGACACCCAGGGCAGCACCGGCACCACCGAGGGCGGCCGCTTCGAGGC10.8849557522123894No Hit
CCGGCTCGATCACGATCGACCTGGCTCGCCGGGGGGGGAGCACCGGCCGC10.8849557522123894No Hit
GGACCCGCGCCGCCGCGGTCAGCTTCTGGCCCGGGGTCTGCAGGCGCAGC10.8849557522123894No Hit
ATGTAACCCAGCCCACCGGCAATTCCGGCGGGCGCATCGCCTGCGGCGTG10.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGAAGCCCGAGCCGGCACCCGAGCCCGAGGCGGAGCGCGATGCCCCGG10.8849557522123894No Hit
GCCTGCCGGCGTGCACGGAGGCGAGCGGCGCGAGCTCGAGGTCGAGGCGC10.8849557522123894No Hit
CCGGCTCGATCACGATCGACCTGGCTCGCCGGGTGGGGAGCACCGGCCGC10.8849557522123894No Hit
TCCCTAGACTCCGCGCCATGCTGCTGAGCGACCGCGACATCACGGCCGCG10.8849557522123894No Hit
GCACCACGATGTGGTCGCCGTGGACATCGACGAGCGCAAGGTCGAGCAGG10.8849557522123894No Hit
GCCCCGGGTGGCCGAGGCGGAATGGATCGGCAATCGCCGGTGGAACCTGA10.8849557522123894No Hit
GTGCTGAGCACCACGTGGCTCTGTTCCACAAGCTGCTGCCCGCTCATCTG10.8849557522123894No Hit
GGTTGGAGGCGCTTTCCTGGTAGCCCCTCAGAGACTCAGTCAGCGGGAAT10.8849557522123894No Hit
CTCGAGAAGATGGCTCGCTTCGTGCGCCCCGACGAGATGCACCAGGCCTT10.8849557522123894No Hit
CTCTTCGACTGGCCGCCACAGGAGCAACCAACGCGGACATCGCGGCCAAC10.8849557522123894No Hit
AGGCGGTGCAGAACATCAAGGCCGCCAAGAAGATGGTCGAGTCGATGATC10.8849557522123894No Hit
TCGCGAAGACCCCCGCCGACCGGCCGACGTCGGCGGCGCACCTCTCGCGC10.8849557522123894No Hit
GCGTCGTGTCGCCGGTCTCGGTGTCGATGCTGGCGACGATGACCGTGTCG10.8849557522123894No Hit
TAGCCCTCGCGCTTCTCCTTGGCCAATTCGCGGAAGAAGTGGCTCACGCC10.8849557522123894No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT10.8849557522123894No Hit
TCGGGCGAGTGGCTGATGCCGTCGGCGCAGCGCAGGAAGAGCATGCCGAC10.8849557522123894No Hit
GCCCTGGGCCAGAACCCTACCAACGCCGAGGTGCTCAAGGTCCTGGGGAA10.8849557522123894No Hit
CTTCAGCAGCAGCGCGTTCACAGCCACGATCACACTCGACCCGGACATGG10.8849557522123894No Hit
GTCGCGCTACGCTCGGTGAGCAGTTCGGTCTCGGCCACGTCGATCATTTC10.8849557522123894No Hit
GGGCAAGGCGCTCGGCGCGCACGACGCCGCACAGGCGCGCGCGGTGACCC10.8849557522123894No Hit
GTCCAGGACAACCTGGTCAGCGGCCACCTGGGCGACCTGCGCGAGCTGCG10.8849557522123894No Hit
CAGCAATCTCCAACACTGCGTCACGCACCAAATATCACATACTTATAAAC10.8849557522123894No Hit
TTGCAGAATCCATGCGATCGCCAATCGGACACAGCCCAATTTTATGTTCG10.8849557522123894No Hit
GTCGCAATACTGTTCATAACAGGCGCGCAAAGACTGGTCCTGTGCGACGA10.8849557522123894No Hit
GTGCTCGAGCGGCCTGGGCCGCCGTGGGCGTCGCCCGACGACTGGGCCTG10.8849557522123894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)