FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003659962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003659962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTTTTTCTGTTAGAAAAGAAATGGCTTGGGTTCCAAGAATAGAAGCAG35.172413793103448No Hit
GACGTCCACCTCGAATACACAAACGCTGAATTAGCGGTCACCATCGCCGA23.4482758620689653No Hit
CCTCCACGTCGGGCAGCAGCACGGCCTCGTTGATCTCGCAGAAGGAATGC23.4482758620689653No Hit
TTCCTTCCTTTATCAGCTTATCCCGCTGCTTCCTGATGGTACCTGCACGT23.4482758620689653No Hit
AAACCACACTAGCCGGTCTGTCAAGGGCTTGAGAATACCATAAACAAGAA23.4482758620689653No Hit
GAAGTCCCGTGGCTGGTAGATCATGACGGGCTCATGGTGCTCCCGCAGCT23.4482758620689653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT11.7241379310344827No Hit
GCCACGGTCAACTTCGTGCGCAAGCGCCCCGGCAAGGAGACGACCGCCTC11.7241379310344827No Hit
GATCGGTGATAACTACCACGAAGAACCGATTCTGGGTTTTTCTATTGTGA11.7241379310344827No Hit
CGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTC11.7241379310344827No Hit
TAGGAAGGAAGAGGTCATCTCGCTCGGAGCTTCGCTCGGAAGGGTCTTTG11.7241379310344827No Hit
GCCAGCAATTTGGCAGGCTGAAGGGGGGAGGATCACTTGAGCCCGGGAGT11.7241379310344827No Hit
GTTCTCACCAGCCTGCTCATAAAGGGCCGTCCCGCTAAAGTTTCCGGTCG11.7241379310344827No Hit
GTAATGCCCATCGCGTCCTCGATAGCATGGGCTGCGCCGGTCTCACCGAT11.7241379310344827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GTGGGTGGATCACACCTGTAATCCCAGCACTCTGGGAGGCCGAGGTGGGT11.7241379310344827No Hit
CACCCGGAGCTCGGAAAGATGCCGTACCTACGATTTCCGGCGGACTTCGG11.7241379310344827No Hit
CCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGGGATTCT11.7241379310344827No Hit
CCTCCACGTCGGGCAGCAGCACGGCCTCGTTGATCTAGCAGAAGGAATGC11.7241379310344827No Hit
TTCTGCCGTCTGAGGTTTTATACTTCAGGTTAGTTAGTTTTTGGAAGGAA11.7241379310344827No Hit
CATCACAATTCATTCTGCCCCTGGGCGCAGCCGCCAGGCGGTCATCTCCG11.7241379310344827No Hit
GAGGAGACTCCATGATCCCAGCCTCCATCCTGGAATCTGAGAGAAGGAAC11.7241379310344827No Hit
GGCTGTGGAGAAAAGGGAATACTTACACACCATTGATGGGAATGTAAAGT11.7241379310344827No Hit
CCCCCAGACCTCACCGCTTCCAGCGCCACGTGGCGAGCCCGTACCGGATC11.7241379310344827No Hit
GGTTTGTCGTAAACAACCTTTCCAGTGGAGAACAGAGAACAGGAAAATCA11.7241379310344827No Hit
GAGTGCATGTCTCTATGGAAATAGGGATAAGTTGCTTAATGGGTAAGGGG11.7241379310344827No Hit
GTTCACCGCCTTTCTCCAGACGACTTTGGGCCATGGTGCAGACGTTTGTA11.7241379310344827No Hit
CTTGAACATCATGCCGCACCTGCCTGCGCGGGCGCCTCGTGCGGCAGCAC11.7241379310344827No Hit
TGACTATCACCATGTGTTCTTCTTCTAGCTTGCCCAATGTCCTTAGTGCT11.7241379310344827No Hit
TTCGAAGACCATTCATTGCACCTGTCGTTGAGGGGGTCATCGATTCATCT11.7241379310344827No Hit
CCATTGTGTGAGCCGCTGCTGTCGCTATGGACCGACTTGTCAACGGTCGA11.7241379310344827No Hit
TCTCATCGTTTTCTGCCGAAGCAAACACTCTACGAAATCATCATATTCTA11.7241379310344827No Hit
TTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGA11.7241379310344827No Hit
TCATGTAGATTAAGCTGAAGATGTGCATGGGGGAAAGAAAAAGACAGACA11.7241379310344827No Hit
GGAAAGAGGAATGCCAGCTCCTGAGCAGGCCTCATTGGTGGAGGAGGGGC11.7241379310344827No Hit
GCACCAGCGCCAAGTCGGTGCGCAGCGACAGGAAGGCGTCGAACGCAAAG11.7241379310344827No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGC11.7241379310344827No Hit
TGACCGACCCCCGCCAGCTTATGGCACTGCGCCTTGGTCAGCGTCGAATC11.7241379310344827No Hit
GAGGAGACCCGGACACCGCGCCTCGCCCCCTGGCCTGCGGCCTCCGAAAA11.7241379310344827No Hit
CATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACC11.7241379310344827No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAA11.7241379310344827No Hit
TCGTGACGGTGCGTGACCACACCATCCTGGGCTTGCGTCTCATTCCTTGG11.7241379310344827No Hit
GGCCTGGTAGGTGCCAGGCCTACAGTTCATGCAACAAACATTAAGCCCCC11.7241379310344827No Hit
GGGGTTCACCGGCCACTTGCAGCGGACCCCGAAGGTGCCGACCGAGGTCT11.7241379310344827No Hit
ACCACACCCGGCTAGTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACCA11.7241379310344827No Hit
ACCTTGGCATAGCGCGGGATCTCCGCACGGATCAGCGCGCCGAACTGCTC11.7241379310344827No Hit
CTCTCTATCCGGGCGGTCCTTCACCATGAGGACGGTGAACGTCAAACTTC11.7241379310344827No Hit
GGTTTGTGATAGAAGCAGTGTTCCAATCCCCTGGCAGGGAAGAGAGAAAC11.7241379310344827No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCTGCGCTGCCGT11.7241379310344827No Hit
TCACAGGACCGCGAGGAATCGGATCAGTTGCCGCCGACGACGTCATCATC11.7241379310344827No Hit
CTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTG11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)