FastQCFastQC Report
Tue 10 May 2022
EGAF00003660161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003660161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316586
Sequences flagged as poor quality0
Sequence length140
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT11630.3673567371898947No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG9500.3000764405248495No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC8820.2785972847820182No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG8540.26975292653496996No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA8450.26691009709841873No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT8310.2624879179748947No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC8200.2590133486635543No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA6990.2207930862388103No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT6760.213528077678735No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG6640.20973763843000004No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT5950.18794261274977414No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG5850.18478391337582836No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG5830.1841521735010392No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG5620.17751890481575305No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT5610.17720303487835848No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA5200.16425236744518076No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG5170.16330475763299704No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG5010.15825083863468378No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT4990.15761909875989463No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG4970.15698735888510548No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG4960.15667148894771088No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC4840.15288104969897595No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA4840.15288104969897595No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT4830.15256517976158138No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT4750.15003822026242475No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG4690.14814300063805727No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG4450.14056212214058741No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG4450.14056212214058741No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG4420.13961451232840366No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4320.13645581295445788No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC4200.13266537370572293No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC4150.13108602401875002No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC4010.12666384489522592No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA3990.12603210502043677No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT3980.1257162350830422No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC3940.1244527553334639No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC3920.12382101545867473No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC3870.12224166577170184No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA3800.12003057620993979No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG3690.11655600689859943No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA3690.11655600689859943No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC3650.1152925271490211No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3610.1140290473994428No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3580.11308143758725905No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC3500.11055447808810245No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT3490.11023860815070785No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3480.10992273821331328No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA3460.10929099833852413No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT3450.10897512840112955No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3430.1083433885263404No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3420.10802751858894583No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT3370.10644816890197292No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA3350.10581642902718377No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA3330.10518468915239462No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC3310.10455294927760544No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC3270.10328946952802714No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3260.10297359959063257Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT3240.10234185971584342No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3220.10171011984105424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)