FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003660477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003660477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGTCTCGAGCAGGATGAGGTGCCATACTGTTTTGGACATACTGGTA39.67741935483871No Hit
GTCTTTACGGCCGCCCTGCGCGAAGGGGCCATTTCCGGCGACGAATCTGC26.451612903225806No Hit
CCTCTCTGCTCCCTCAGAGGTGCCTCTTCATGTGCAGGGCCAGGTGGTCA26.451612903225806No Hit
GTTCAGTGCCTTCAGCGCAGAGCGTGGATGCTCTGGAATCACCCGGACCC13.225806451612903No Hit
GCCAAGCCTTTGAAGCCAATGGTCATATCTACACCTCAATCCTGTCTCTT13.225806451612903No Hit
CAAAAATTTTGTTTCTTTTGAGACGAGGTCTCACTCTGTCACCCAGGCTG13.225806451612903No Hit
GTGCTGGTCAGGCTGGTTTCGAACTCCTGACCTTGTGATCCGCCTGCCTC13.225806451612903No Hit
GGCATCGCCCGATAGAGCCGCTTGGTCGTCGCACGGGTCCGCACGTTCTG13.225806451612903No Hit
GCCCCGACGGTAAGTCGCACCCGGAGATCACTGACCGCCTGTCTCTTATA13.225806451612903No Hit
TTTCGAGGCAGTCGACGCCAACGAGGATGCTGTCTCTTATACACATCTCC13.225806451612903No Hit
AATTTGACTTTCGCAAGAAACTACATTAAGGTAGTGGTCCAGCTGTCTCT13.225806451612903No Hit
GCCAAACACTCTAGGGGGATGCACTGTAGGTCCCTTGGAGCTGTCTCTTA13.225806451612903No Hit
TTGGGAGGCCGAGTCAGGGGAATCGCTTGAACCCGGGAGGCGGAGGTTGC13.225806451612903No Hit
ATGCGATGGCGTTGACGGCGGCGTCGAGAAGCGTGAGGTCCGTGACGCCC13.225806451612903No Hit
ATCTTGTTGCCCAGGCTGGAGTGCAATCCCATGTACTCCTGTCTCTTATA13.225806451612903No Hit
ACCAAGAACTGAGGCAGGACAGAGAAGCTGTCTCTTATACACATCTCCGA13.225806451612903No Hit
TTTCTGGCCTGTGGCTTTCTGGGCAGGAACCTTCTGGGCTGGAGCCTTTT13.225806451612903No Hit
GGCAATGATGGGTGAGCAAAACAGTTGGCGCCTTCGAGACCAGTTTGGCG13.225806451612903No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC13.225806451612903No Hit
AAAGAGAACAGCATTGAGTGAGGTCTTTTTTCTACATCTCCTTCTGTCTC13.225806451612903No Hit
GGGTTCAACTATCAGATACCGGCTCAACCGCAGCGGAGACCGTCAACTCA13.225806451612903No Hit
CTGCTACAGCTCGCCAACCGATGAGGGTAAGAGCCAAGAAGCACGCCGCG13.225806451612903No Hit
TTTATGTCTAGCTAAGGGATTGTAAATACACCAATCAGCACCGTGTGTCC13.225806451612903No Hit
GCCCAAGACAACTACGCGTTCTCAAGCAGGTTTAATCCCGGAATCAATAG13.225806451612903No Hit
GGAGTAGGCTGCCGAGGATCTGCAAAGCCACATGTCTGTCTCTTATACAC13.225806451612903No Hit
GCACCTCACGGTTCGCCCCGGTGCGCTTCGCCTGCCGGCCGGAAGCCGTG13.225806451612903No Hit
CAGCACACCCAGCACGCTGAGAGGATCGGGGCGCTGACATCCGCACCGCC13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)