FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003660867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003660867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length146
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCTCACCATCCTCTCGCGGAGCGACGCCGGATGAGCGCGGGCACCGC416.0No Hit
AACACCGAGCTGTGCATCGCCGAATGCATGAAGCAGCCCGTGTGGCGGCT312.0No Hit
AACTTGGCCGGGTCGCCGCCGCGCTGGATGTCTTCCACCACCGCCTTCGG312.0No Hit
CAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAAG28.0No Hit
CGACATAAGAAAGCTGAAATAACACTGGTTGACCGTAATCAGACCCACTT28.0No Hit
GTTCGACCATGGTCTTGCAGGCCTCGCGTGGGGTACGCTGCTCACACAGG28.0No Hit
TCGTCGTCGTGCACCTCGTGATGGTGGCCCTGGTGCCCCGCACGCTCCTC14.0No Hit
AGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCATTGTTATGAT14.0No Hit
CGGCATCCCGGCATCGCTCAGCACGAGGACGTCCTGGTCGTGCGCGAGGG14.0No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14.0Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTGGACCGTCGGTATTCGCCCGATGTACAAGTGGACGCCAATCATGA14.0No Hit
ATTCAGTGCAGACCCATTTCCAGGAGTCCCCCGTACATGAGCAACATGAA14.0No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTAACACGGC14.0No Hit
GTACTTGGCTACATTCCCGGACCCGACCGACTGAGCGAGAGTGCCGTCTC14.0No Hit
TCAGAGGACGCCGCGATATCGTAGCAACGCCGTGGTAGCCCTTCTGACCG14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)