FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003660868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003660868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length146
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCGCATCCGCCAGCGCGGCGATCTCGGTCGGCGTCACGGCGCGGAGC416.0No Hit
GGTCAACGCCGAGGTGCTGGCGTGGATCGTTCGCCGCGCCAGCAACCAGT312.0No Hit
AGGTATGTCCGTGGATACGGCGGCTGCCTTCCACTTCGATCTCGCGCTTG312.0No Hit
GTCATAAGGCAGATCGCGCTCTTCCACCAACAGTTGCCCATTGTCATCAC28.0No Hit
GTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGC28.0No Hit
GTTCTACACCGTCCCATCGCGGCTGATCGGCCATCGCCTGCGCGTTCGGC28.0No Hit
GCTCGCACGCGGCGCGAGGGCTCGTCGAGCTTCTTCGAGATCAGCGTGCG14.0No Hit
GTCGCCAGCCTGCCATTGTTGTGCGAGGGTAATTTTGTACTGATTGTTCT14.0No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA14.0Clontech Universal Primer Mix Long (95% over 21bp)
TCAGAATGTGAAGCAGGAGCCGGACATGACCACGCTGGTCCTGGGCGACA14.0No Hit
CGGCCTAGGACACGCGCACCACGCAGCGGCTCCTCGCCGCGCTCGGGGTG14.0No Hit
GACCGCGTCTCCAGGAGATGCTCGAGTCTGGCGAACTCGGACCCGGGATC14.0No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA14.0Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGTCTCTTATACACATCTGACGCTGCCGACGATTTCAGTAATCAGTG14.0No Hit
GTGCTGCAATTGGGAGGTCAGCGGACCATCCGGCACCTTGATGCTGAAGA14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)