FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003660999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003660999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGACGCGCAT46.896551724137931No Hit
ATGCGGTAGACATGGCACGCGCCGATCTCGCTCTCGGCCTTCAGGCCTTC35.172413793103448No Hit
ATGTAAAAAACAAAACAAATGAGAAAAAACACTTCAATTACTACAAAGAC35.172413793103448No Hit
GGGGGGTGACTGGATCTTGGGGGTGGACTTCCCCCTTACTGTTCTGACAG23.4482758620689653No Hit
GGTAAGGACCCGGGGACCTTTAGGAGTCAGTAGATCCAAGTGTGTGCTTT23.4482758620689653No Hit
ATTCCGTTCCATTCTATTCCATTTCATTCTGTTAGATTCCATTCCAATCC23.4482758620689653No Hit
GCGATCAGCAGCGCTTCGCCGATGGGCTGCACGATGCTGCGCGTCAGCAG23.4482758620689653No Hit
GTCTAGATCTCCTGACCTCGTGAACCACCCATCTCAGCCTCCCAAAACCC23.4482758620689653No Hit
GGCATGAGCCACTGCACCAGGCAGAAATTTTTTAATCTGATGAATGTGAA23.4482758620689653No Hit
GATGGGCAGCGTGCAGCCGCGCGAGAGCAGGTCCACGATCATCGAGCGCG23.4482758620689653No Hit
GATGGGCAGCGTGCAGCCTCGCGAGAGCAGGTACAAGATCATCGAGCGCG11.7241379310344827No Hit
CCATAAAACACCTGGGTAATCACCCAGCCAGGTGGGAGCTCCTGACTCAC11.7241379310344827No Hit
AGCTGCAGCTCAGTCAAAATATTTTCCATTTGGGTTTAGTTTCTGCTTGA11.7241379310344827No Hit
ATAGGCAGCAGAAAAGCAAAAGAAAAACAGCTCTCTCTCTCTAGAGAGAG11.7241379310344827No Hit
GGATGATGCTGGCCTCATAAAATGAGTTAGGGAGGATTCCCTCTTTTTCT11.7241379310344827No Hit
GGGCGGGCGTTTCGTGTTCACCGTGTGGGACCGCATCGAGGACAACGCCT11.7241379310344827No Hit
AGGCAATCCTAAGCAAAAAGAACAAAGCTAGAAGCATCATGTTACTTGAC11.7241379310344827No Hit
GGTTCCATAAACACTGTGAATCTCTCTGACTCAATATTACTCTCCTGTAA11.7241379310344827No Hit
CACTATTCCTAGAGACACCTCTATTGATGAGAATTTGTCCTAATACAAAA11.7241379310344827No Hit
CTACTCCAGCAAGACAGTTTTTTTAATTATTTGAAAAGTCACTGCCAAGC11.7241379310344827No Hit
ACCCAGAGGAACTCGGGTGAAGTTAAATAAATAAGGACCAGCCCTCTCGA11.7241379310344827No Hit
TCCTGGCACCATCGGCAGCCTCCCTATCTCTCTCCTTTGTGCTCCTGTTC11.7241379310344827No Hit
GTATTTCTGGCATCACAAACTGAGCCAGAAACTCAGTGCCTCCCATTTCT11.7241379310344827No Hit
GACCACAGCGCAATCAAATTAGAACTCAGGATTAAGAAACTCACTCAAAA11.7241379310344827No Hit
AATCATCTGAGGTCAGGGGTTTGAGACCAGTCTGGCCAACATGGCGAAAA11.7241379310344827No Hit
GGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTT11.7241379310344827No Hit
GTCCTCCTATCTCAGCCTCCCAAACTGCTGTGATCACAGGCATGAGCCAC11.7241379310344827No Hit
GTAATGCAATGGAATAGAATGGAACGAAATTTCACGGAATGGAATCAAAC11.7241379310344827No Hit
TTATTAGTCTGGCTAGTGTTCTATCTATTTTGTTAATCTTTTCAAAAAAG11.7241379310344827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GAAGTGGACATGGCCGTCGGCTACTTCCCGGCGGTGATCGCCAGCCTCGC11.7241379310344827No Hit
GACATGTAGGCCAATGGAACAGAATAGAGACCTCAGAAATAAGGCCACAC11.7241379310344827No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG11.7241379310344827No Hit
GTCTTAGGAGTCTTCCAATGGGGTCTTACCTTTTCCCTTTGATCGCCGTG11.7241379310344827No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACG11.7241379310344827No Hit
ATTTTCACGATATTGATTCTTCCTACCCATGAGCATGGAATGTTCTTCTA11.7241379310344827No Hit
GCCTGGGGCTGACCGACAAGGCCAACTCCAACATGCGCCAGCTGTCCGGC11.7241379310344827No Hit
CGTTGAGTCTGAGGGCAATGGCCGTATGTGCCTGACTCTGCCGGACACCC11.7241379310344827No Hit
ACCGTCGATCTAAGCAAAATAAGATGCATAAAGGATAAACATCACTTGCA11.7241379310344827No Hit
GAATATTCCATGCTCATGGGTAGGAAGAATCAATATCGTGAAAATGGCCA11.7241379310344827No Hit
GCTTCCACCCTCTGGAATTTATATGCATCCAACTCCCAAAGAATATGCCT11.7241379310344827No Hit
CCTTCTTCAGAGATGGGCTCAGTGGGGAGGAACAGCAGATATTATGAGCA11.7241379310344827No Hit
TCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCACGAGGTCAGGAGTTC11.7241379310344827No Hit
GGTAGCAGGTAGATCACCTTCCTGGGAATTGGGACCCAGGATCTCCATGA11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)