FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length146
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTTTATCAGTACAATCCTGAAGACAAAATACAATCAAAGTGATGGCT45.263157894736842No Hit
GGTTTTTCTAGTGCTTGCACAGTGGCTCAACACACTTCTTTATACATCAT33.9473684210526314No Hit
ACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTA22.631578947368421No Hit
TGGTAAGACTGGTCTTTGGAACTTGCCCACTCCATTTGAGTGGAAGTGTG22.631578947368421No Hit
TCTCAAACCTGTGTTAGGCTACGGCAGCTGCTTGTTTCTCTACGTGAAAC22.631578947368421No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT22.631578947368421No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAA22.631578947368421No Hit
GTCTGAGATTCACACACTCCGTGAAAGTGTACTTGTCGTTCCTTTTCATG22.631578947368421No Hit
CAAATACAGTGAATTGTTACAGCTAAAGGACTCAGTTACTGAGTACAGAG22.631578947368421No Hit
GTACATGGGAGGTGCATGCCACCACGCCCACCTAATTTTTTTATCTTTTA22.631578947368421No Hit
TTGTATCTAAGTACAAAACCCTTGCTCAGAAGCTGTATCAGCATGAAATC11.3157894736842104No Hit
CTGTAAACAAAACAAAAACATAAGGAAACATGAAACTTCAACATTCCAAA11.3157894736842104No Hit
CCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTA11.3157894736842104No Hit
CCTTTTATAAGGAAGCATGCGTGTTCAGGACACACATCAAACACATCACA11.3157894736842104No Hit
GATGGTAAACCAAAGCAGTTTGCGTTTGTGAATTTCAAACATGAAGTGTC11.3157894736842104No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.3157894736842104Clontech Universal Primer Mix Long (100% over 23bp)
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTGCCGAGTT11.3157894736842104No Hit
GTAATAAGTGAAATATTAACATAAAGTACCCAAAGAGATATAGTGATTTT11.3157894736842104No Hit
GCCCTGATGCGGCCAGACCCGCGCCTCAAGTGGGCGGTGCTGGTGCTGGT11.3157894736842104No Hit
CTGTTCAGTGTATGCAACAGTGAAGCAGTTGGAATTTCATAGAAAGAATA11.3157894736842104No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT11.3157894736842104No Hit
CTCCTTACGTCGTATCCTCTTATCCTCACTCCCTGGTGCCGCTGTAACAT11.3157894736842104No Hit
GTTTTTGTCCTCTGCAGCAATGTTTTGCTATGCTGCAAATGGTTGTGACT11.3157894736842104No Hit
GCATGATCTAGATCGAGTAAAGAAACCTGAATGGGTTATCCTGATAGGTG11.3157894736842104No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG11.3157894736842104No Hit
ACCTAGAAGATAAAAACCGTCCTTTCTACTATGACTGGCACAGCCTCCAG11.3157894736842104No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.3157894736842104Clontech Universal Primer Mix Long (96% over 26bp)
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT11.3157894736842104No Hit
CACCGGTGGCCCGGCAGAGCCGGCTCCACGGTGCTTCCCGAAAAGACCGT11.3157894736842104No Hit
GCTGTGGCCGGACAGCTGCTGCCCAGCACATGGAGCCTGCACGCGCACGG11.3157894736842104No Hit
GGTCGGTTTTGGTTGCTGGCTTCCAGTTTTCGGCACTAATTACTGGCAGA11.3157894736842104No Hit
CTTCTTACCAAAACCTAAACTGTGAAGGCAGCACCCAAGCCAAAAGCCTC11.3157894736842104No Hit
GTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGA11.3157894736842104No Hit
CCTTCAGGTTTGGGACGGCATCCAGGCAGCTGGGCTCAAATACTTGGTTT11.3157894736842104No Hit
ATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGT11.3157894736842104No Hit
AAAGGAACTTGTACTTTGCTGACCTTGATGTTTGTGTTCAGATAATGGAA11.3157894736842104No Hit
GGTATCAACGCAGAGTACATGGGATCGTCGGTAAATACGGGACCCGCTAT11.3157894736842104No Hit
GCTTCCTGAGTTCCTCAGAAGGAGATGCTGGTGCCACACTTCTTGTATAG11.3157894736842104No Hit
ACACTAAGGGCGACTACCAGAAAGCGCTGCTGTACCTGTGTGGTGGAGAT11.3157894736842104No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.3157894736842104Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGAAGAAGGCTAACCTCGAAAGGGAAGAGAAGAAAAGAGATTACCA11.3157894736842104No Hit
ACTTGAAAAGGGTTCAGTCCATCAAACACATACTTAACCAAGCACCTGCA11.3157894736842104No Hit
ACCCAGTTGTTAAATAGAACATTTTGAAGGTGAACACACACCCTAACCCA11.3157894736842104No Hit
GAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAG11.3157894736842104No Hit
CCTTTGTAATTTGGTTCTCTTTAATGTACTTGATTCTCTTAAGTATTCTG11.3157894736842104No Hit
CAGTAGATCCATATTAAAACGAGATGACAGAAGCCAAAGTAATTATGGCA11.3157894736842104No Hit
CCGTCTACGCTACCGTAACGAGTACGCGATGGCGCGTGAGGCAGAAGAGC11.3157894736842104No Hit
ACTTAGATTCTCAGAAAATAATGTAATTTAAGTTTTGCTGTGAGGTCAGA11.3157894736842104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTC11.3157894736842104No Hit
CTTTAAGTGTGTGATTCAAGTTCCATTCCAAATTAGCTTTTAGATCTAAA11.3157894736842104No Hit
GAATAAACTCCCGCCCGCTTGCAGCGTGATTCGTGCTGCGCCGCCATAGG11.3157894736842104No Hit
ATGTAATGCAGTGCTATCCCAATATTTTCAATAAAGGACTTTATGCATTC11.3157894736842104No Hit
GGAGCATTCAAATGCTTTTTTTATTATAGTGGAATTTTGTTTCAGGAAAC11.3157894736842104No Hit
TTCTCACAGAGCAGTCAGGTGCACCTCCACCAACAATTCACTGAGGATAA11.3157894736842104No Hit
ATATTTTTCTTGTTCACGAAAGTTCCTGTGGTGAGCAGTGAATTCTCATA11.3157894736842104No Hit
GCAATATGGAGAGGCGTGTCTCCGTCCTCATCTGCACGGGTGGCCATGGC11.3157894736842104No Hit
GCCAGCCTCTGTTGTTTTTTTACTTTTTATTAAAAGCCATTTAATTAGGT11.3157894736842104No Hit
CCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAAC11.3157894736842104No Hit
TCGTTATTCGCAGCTTCTCAGTCTTGCAAGAAGCATCTTTGCATCTTCCA11.3157894736842104No Hit
TGTCATAACAGAATTTTCATTTTGGTTAGTATCAATTCCTAGAGAACTAG11.3157894736842104No Hit
GAATTTCCTTGGGAAATAGTTGAATTTTTTCCTAACCTTCTCAGGGTGGG11.3157894736842104No Hit
ATTGTATTCTGTGCTGCTTGATGCATTTTCTCTAGTTTTTCCAGAGTCAG11.3157894736842104No Hit
GCCCTGGCTCCCGAGGCAGGCAGGCAAGGTGACGGGACTGGAAGCCCTTT11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)