FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATCACCCGTCTCTGTGGCGTGATGTTTATCGGCTTCGCCATTAACG36.8181818181818175No Hit
AGGTCACCGAGCCCGAGGCGCGCGCCGCGCTGGCCGAGGTCGACGGGATC36.8181818181818175No Hit
GACAACATGGGCGTGCCGGGCTGGTTCCTCAACATCGTGATCATCTCCCG24.545454545454546No Hit
CGCTGACGCCGTGCACGCCATGACCGGGCGGTCCGTGCGTGAGGGCTTCC24.545454545454546No Hit
GGCCAGGCTGATCTCAAACTCCTGACCTCAAGTGATCCGCCTGTCTCTTA24.545454545454546No Hit
GCCTCCAGTTTCTCCTTTGCAGCCATGTTGTTTTTTTTTTTCTTTTTTTT24.545454545454546No Hit
CGCCCTGGGGGCCGCCCGTGCCCACGCCCTGAACGGGCACAGCCGCTGCG24.545454545454546No Hit
GTCCTAGCTAATGAGAGTGTGTCAGAAATACAGATGGCAGAGAGGCAAGC24.545454545454546No Hit
AACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGT24.545454545454546No Hit
GTTCCGCACTTCGGTACGCGCGAATCTTGCTTATGGGCTCATCACGGGCG12.272727272727273No Hit
GTCGCCGATCATGGCCAAGACCATCTTTGGTCACATCGCGCGCCTGCCGT12.272727272727273No Hit
CTAGCCCACAGGGAGTACGACTCCCTAAGGAAAGGACGAAAGGCTGCAGC12.272727272727273No Hit
AACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCCGCCGGCGCG12.272727272727273No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG12.272727272727273No Hit
GTGCTCGACCTGACCCAGAGCGAGGGGGCGCCGCTCGAGAAGGCGCCGGT12.272727272727273No Hit
CAAAATCTCTTAACCTCTACACGCCACGCTGCCACCTGGTGCTCAGGCAC12.272727272727273No Hit
CATCCGCACGGTCCCGCCGCCAAAGGTCCAGAATGGATCGGCAATCCCGC12.272727272727273No Hit
GGCTGGCCGCGCGCTTGGCCGAAAGGATCTTCTCGTCGTTATCGAAGGCA12.272727272727273No Hit
CAGGTAATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCAAG12.272727272727273No Hit
GTCTTGGTACACGTGGTTCGGCCCAGCTCCACGTCCAAGAAGTAGTTCAC12.272727272727273No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT12.272727272727273No Hit
CTCCATTCCTGGAGATCTGGACACTCAATTTCCACTTTTCTTCTCACCTA12.272727272727273No Hit
CGATTAATCGCCCGATTTCATATCGGCTTTCTGGCGACTTTTAACGCCTG12.272727272727273No Hit
GGCGTTGCTGGTGATGCCGCGCCCGCCGAACTCCGCCGCCAGCGCCCGCA12.272727272727273No Hit
GTACAGCAGCACGAAGATGATCAGCAGCGTGAACGGCACCACCACCTTCA12.272727272727273No Hit
CAATCCATCTAGGAATCCATCATTAGAACCAAGCGTTAGGCCTTTTTCAG12.272727272727273No Hit
AGGCATTCACCGGCCCGCCCCTGGCGAGCTGGATGTGCGGCACCTCCAGC12.272727272727273No Hit
GAAGACCGGCCTGCCCGACCGGCAGGCCCTCGCCCGCTGGGACGGCCGGC12.272727272727273No Hit
GGGTCCGCCCCCGGGGCCGCGGTTCCGCGCGGCGCCTCGCCTCGGCCGGC12.272727272727273No Hit
GAGTCGTCGTTCCCGGGGCTCCTCGGCAACGTCGTCGCCGGGCGCGTCGC12.272727272727273No Hit
GCCTACGGCGTGACCACGCTCAAGCGGCTGGGCACGCCCGCGCTGAAGGA12.272727272727273No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA12.272727272727273No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGG12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)