FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGCAGCACGAAGATGATCAGCAGCGTGAACGGCACCACCACCTTCA410.526315789473683No Hit
GTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTG37.894736842105263No Hit
AGGTCACCGAGCCCGAGGCGCGCGCCGCGCTGGCCGAGGTCGACGGGATC25.263157894736842No Hit
GACCGGACCCAGCAGTTCGAGCTGCAGCGCGAACATGGCGAGATGCAGCA25.263157894736842No Hit
CTTGAGACGTGCCTGGCGTCTCGACGCCGTCGAACTGGTCCTCAACCCCA25.263157894736842No Hit
GCCCCGACTACCTGGCGCGGCACGGCACGCCGATGCACCCGCGCGAACTG12.631578947368421No Hit
CACGCGGGGGGCTCGCTCGTCATATCGGCGGAACCCACACGAAAGGAGAA12.631578947368421No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA12.631578947368421No Hit
AGCTGAACGACGCCGAAGCTGAGCTGCGCAAGAGCCGCGCCGAGACCGCC12.631578947368421No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC12.631578947368421No Hit
CTTGAACCTGGGAGGCAGAGGTTGCGGTGAGCCGAGATCATGCCATTGCA12.631578947368421No Hit
GGGAGATAGAAGGACAAGACCTCAACACTAATTTTTTAATGTGAAAGGGC12.631578947368421No Hit
AGCTCTACTACGAAGGTAAGTGGGTGCCCGCCGATACCAAGGCCGCCGAG12.631578947368421No Hit
GTGCCTACTGTCTCCAAGGAGTGGGAGAGGACAAGCATATGGCAATTTTG12.631578947368421No Hit
CACATAAGCACCCACTTCTTTTTCTCTCTACTAAACCTTTCACCTTGTCT12.631578947368421No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA12.631578947368421No Hit
CGCTGACGCCGTGCACGCCATGACCGGGCGGTCCGTGCGTGAGGGCTTCC12.631578947368421No Hit
GCCCTCACCCTGCTCGACGAGGTGGGAATGGACAAGCTCAGCACGCGGCT12.631578947368421No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT12.631578947368421No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT12.631578947368421No Hit
GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTAT12.631578947368421Illumina Single End PCR Primer 1 (97% over 39bp)
CCTCTCCAGTGTATCCACAGCCCCGACCTTTGATGAGAACTCAGCTGTCC12.631578947368421No Hit
CATCCCGATCGCCTTCGGCCCCTTCCTGGCCGGCGCGGGCCTCGTCTGCC12.631578947368421No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG12.631578947368421No Hit
ATGATGGGCTCGTCAAGTTTAACCTGAGTATTCCGCACGTGCAAAACTTT12.631578947368421No Hit
ACCCTCTACCCTCTGGTAGGCCCCAGTGTCTGTTTTTCCCCTCTATGTGT12.631578947368421No Hit
ACTTCTTCGGCCGCATCCGCGCGCTGAAGGACCAGGCGCTGGCGCAGTAC12.631578947368421No Hit
GGCAAGAAGCCCTTCAAGAACGCCACGCCGAGCAACAGCCCCGACATCGT12.631578947368421No Hit
ACGTTTCCCATGCGCCTTATGGTTCCCTTGTCATCATTTGTAACGAGATG12.631578947368421No Hit
GATCTGGACCAACGCGCTCGGCACCAACATCATGACACGGATGCAGGCCG12.631578947368421No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)