FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length146
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCGCGGCAGAGACCTGCGTTCGGCGGTGCAGGACATGCAGCGCGTCGT410.526315789473683No Hit
GCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTC37.894736842105263No Hit
GCCCTCGCCGGACCGGTACACCGTCTTCTGGCCGGTGAGGCCGATCTCGC25.263157894736842No Hit
GATGAGCAAGGCGAGGCAGAGACAACCTCAGAAGGCGGACAGAAGCCCCG25.263157894736842No Hit
GGGCCCGGCCGCGCGGACCAGCTGCTGCACCTGGGGATGGCTGTCGAGGA25.263157894736842No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG12.631578947368421No Hit
GAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGA12.631578947368421No Hit
AGACATAGGCGTTCAATGGGTCGGGCTTGGTGCCCTTGCCTTGGGAAAAC12.631578947368421No Hit
GGTCAGTACAGGTCCCGGGCTTATGCGGTTTCTATGTCGACGTTGCGCCC12.631578947368421No Hit
ATCGGAGACTTTGCTCGTCATCGGCGTCGCGCTGTCATGCGCCGTTTCGG12.631578947368421No Hit
CGGCGCGAGCACGCTGGTGCCGTTGCAGATGCCCAGCATCTCTCGCTCCG12.631578947368421No Hit
CCTTGATGTTAGCCATGCATGACCCTCAAATCCCGGAATCTTTGAAAGTA12.631578947368421No Hit
ACACGCTGCTGGGCCGCATGCTGCCCCAGCTGGTCTGCCGCCTCGTCCTC12.631578947368421No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGG12.631578947368421No Hit
GTCCTTTGCAGAAACATAGATGGAGCTGGAGGCCATTATCCTTAGCAAAA12.631578947368421No Hit
CTCCAGATCCTGGCGCTTCTGCTCGTAGGTCTGCTCGATCTGGTTGAGCG12.631578947368421No Hit
GTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTT12.631578947368421No Hit
GAGACACCACGTGATGATCGGGTGTGATAAGCTCTACGTTCACATACAAC12.631578947368421No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA12.631578947368421No Hit
GGCTCCGAGCCCGCATGCAGCCGCGCGCCGTCGCGCCGCGCCAGCAGCGC12.631578947368421No Hit
CACTTGGGGGTCGGCTGCGGAAGACGGGGAGGGAACAGACTCCAGGAGCT12.631578947368421No Hit
ATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATC12.631578947368421No Hit
CATCCCCATGGCTCCCTTTTATCAGCCTCTAATTCTTCTCTGAACCATTT12.631578947368421No Hit
CTTCCTCGACCATGCCTTCGACGCGCCGGATGAAGCGCGAGCTGAGCGCC12.631578947368421No Hit
GCCAGTGCATCTGCAGGCACCGGCGCGCCCAGCAGCAGGGCGAGGGCGTT12.631578947368421No Hit
CGCCCTGCCCATTTTACATCTTAAAGTGTATCATCAGTGCTCTTTTTTTT12.631578947368421No Hit
GGGCCTCGCACCGCCTCTTCCATGCCGAGGTTGAGCACCTGGTACGAGAA12.631578947368421No Hit
AGCCGGGCCTTGGCGCCTGCGTCCGCGAACACGATCTGGCGCATGGCGGC12.631578947368421No Hit
GCTCGGTCTCGCCTTGCTCGCGCGCCTCGGCCGCGTGCATGTTAATGCAG12.631578947368421No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCCTGTCTCTTAT12.631578947368421Illumina Single End PCR Primer 1 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)