FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA44.545454545454546No Hit
GAGTGGTTCACTTCATCGACCGGTCGGCCGGCGTGTGGGCACTGCTTGCG33.4090909090909087No Hit
ACTGTATTCCTTCTCAAAGTTGTATCAGCCTATGCCCAGGAATGAACAAG33.4090909090909087No Hit
GTATCACACTGATGAGTGCATATTTAGCTACCAATTAATTCTGGCCTTGA22.272727272727273No Hit
GTGCAGGACCACAGGACGTCCTGGTCGATGATGCCCTCATCGCCGTCGCC22.272727272727273No Hit
GTTAAGCGTGGAGGTGTCGTGGGTCAAGGGGCTCGCGCCCTTGTTGGATG22.272727272727273No Hit
GATCACGTCCACGCCCGTGGCCGCGATCGCCCCGATCGTCTCGAGGCTCA22.272727272727273No Hit
GCCTGGGGAACAGAGCGAGACTCTGTCTCAAAGAAAAAAAGAAAAAAAAG22.272727272727273No Hit
GGTTGGGAGTTCCAGACCAGCCTGGCCAATATGGTAAAACCCCGTCTCTA22.272727272727273No Hit
GAATGCAATGGAATGGAACGGAAAGGAATGGAATGGAATGGAATAGAATG22.272727272727273No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG22.272727272727273No Hit
GTGCCAGCACGTCGATCAGGGCCGGGCCATCATGCGCCAGCGCGGCCTTG22.272727272727273No Hit
CTTTAAATACTTTTGCAACTCTGGCAATACCTTTTCAAGCTGTCTCTTAT22.272727272727273No Hit
ACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCC11.1363636363636365No Hit
CTACGTGTCCGATGACGGCGTGACCGAGGAGCGTGAGCGGGACACGGAGA11.1363636363636365No Hit
GGTTTGGATGCCAGACAAATATCTAAATCTAACCCTAATCCACTGCTTAT11.1363636363636365No Hit
GTCATTTACAAACTGCCATGCTAACTTATGATTTCCCTTTCAACTGGTAT11.1363636363636365No Hit
GGGGGCGGGCCGTCCCCGGTGCGGACCTGCGCCGCGGGGGCGTCGCCCCG11.1363636363636365No Hit
CACGCTCACCGGCCGGTTGATCGCGCGGCTGAGCTCGGCCAGGAGCGGGT11.1363636363636365No Hit
GCCCAGAAGGACGCCGGCGTTGTTCGCCTCCGTGCCGGGGATGCAGTGCA11.1363636363636365No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT11.1363636363636365No Hit
GAATAGAATGGAATGTAACGAATTCTAATGGAATGGAATTGAATGGAATG11.1363636363636365No Hit
CCGCAGGAGAGCCAGTACCGTCAAACAGACGGCGAACCAACCTGTCTCTT11.1363636363636365No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA11.1363636363636365No Hit
GCGGGGACGTGCGCGTTGCGGCGCAGCCCGGACGGGACGAGCCCGCGCTC11.1363636363636365No Hit
TGTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGC11.1363636363636365No Hit
AATTAGCTGGGTGAGGTAGCATGCACCTGTAATCCAAGCTACCTGTCTCT11.1363636363636365No Hit
GAGAGGAAACTGACCTACTAGCGATTCCTCTCACGCACACAAAGGTTGGA11.1363636363636365No Hit
CACCTGGCCCGCCCCGCCCAGCGAGCCGATCGACGTGCCGGCGGCCGCGA11.1363636363636365No Hit
TATCAATATTTTTCACATTTGTTCTTCTGTCCAACCTGCTTGGCAACAGT11.1363636363636365No Hit
GGCAGAAAGATGTATCGGATACAGCTGCTGCCCCCAAAGAATCTCCAGTC11.1363636363636365No Hit
TTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGC11.1363636363636365No Hit
GCATTGATGTTCATCAGGGAGATTGGTCTAAAATTCTCTTTTCTTGTTGT11.1363636363636365No Hit
GAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAG11.1363636363636365No Hit
GTCTGCGGCGTCGAAGCGGGTATCGTCGTCGCGGTCATGGCTGTCGTTCC11.1363636363636365No Hit
GCCTGAGATCACCCGCACCCTCACCCGAATCGGAACATACCAGGCACACT11.1363636363636365No Hit
CCAATTTCTCGGTTATAAGATTGAGCATTGGAAATACTTACTAATACTCA11.1363636363636365No Hit
GGTCTCTGTGCTTGGAGTTTGGAGAAATAAGTAGCCTGCCACAAATAATT11.1363636363636365No Hit
GCTTGTGTCAATGCAAGAATGCAAATTTAGCAGTCTCCAAACTGTCTCTT11.1363636363636365No Hit
CAAACGGGTCTAACAAGCGAAAGATCGCGAGTGAGATCAGGAGGTTGGAA11.1363636363636365No Hit
CTGCTGTCGATGGCCCTTCCGTCTCCGCTCTGGTGGAGTGAAGCGCACTA11.1363636363636365No Hit
GTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATTCAGAGCACTGG11.1363636363636365No Hit
CCTCGGACACGGTCTCCGGCATCACCCGGGCGGGCACGGGCCGCGCCCAC11.1363636363636365No Hit
CCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC11.1363636363636365No Hit
TCTAATGGAATGGAATGGAATAATCCACGGACTCGAATGCAATCATCATC11.1363636363636365No Hit
GATCCTGCTCTTCACCGGCGCCTCCACCGCGGACGGCACCCAGGCCAGCC11.1363636363636365No Hit
ATTCAGGTTGTTCCATTCCATTCCATTCCATTCCACTCCATTCCATTGCA11.1363636363636365No Hit
GCATATGTTGAACCAGCCTTGCATCCCAGGGATGAAGCCCACTTGATCAT11.1363636363636365No Hit
GATCGGCGCCGACGAATATCGCCGCCAGGTCGAGGAGGAGTTCGTCGCGG11.1363636363636365No Hit
GCGCACAACCGCAGTCTCACTTTCTCCAAAATCATAACTTCCATCGGCAA11.1363636363636365No Hit
GGTATTGGGTATTCTGAATAAGCCGGCTTTGAACTTTCTTCCCACAAGAG11.1363636363636365No Hit
GCGCCAGCGAGGTGCAGACCGAACAGGCGCGCCAGGACCTGATGCTGCGT11.1363636363636365No Hit
CCGCTCAGCCGCCCACGCGAGCGTTGCGGTTGCCGATCAGCTTGAGGCGC11.1363636363636365No Hit
GTATCCTAGTATGTCTCATCAGGCTGTTTGAAACTGTTGATGTCAGAACA11.1363636363636365No Hit
GTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAA11.1363636363636365No Hit
CTAGAGATGCTCCCTGACTGTCCTGGTTTTATGTTCTAGGCAATCGTGTG11.1363636363636365No Hit
GGGCATCTCTGAAAGAAAGGCAGCAGCCCCGGTAAGGGGCTTATAGATAA11.1363636363636365No Hit
AGCTGCCGCAGCGCAGGCGGCGCGTGAGAAAGCCCAGCGCCTCGTCGTCC11.1363636363636365No Hit
GCCCCACTGCGGCACCATCACAATGCAGCGGATCTCCTTCACCTGGGGGT11.1363636363636365No Hit
GTATCAACGCAGAGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCAT11.1363636363636365No Hit
GGATGTGAGCTTGTAGGGCTTGGAAATGTCAATGAATAGAATTGTCTGAT11.1363636363636365No Hit
CGGGGAAACAGATGAAGCGATCGGTTGGGAATCCCACCCGAGAGGGATCC11.1363636363636365No Hit
TCTTGAGACTGTTGTGTTTCTCTTAATTGGAATTGAAGTTGTTCTTTATC11.1363636363636365No Hit
AGTGGGACCTCTACATCCAGGCGATCCCCTACGAGGAGGGCAAGACCTAC11.1363636363636365No Hit
GTCATTGCCTAGCTTCAAAGCTACAAAGGACAGGCTGACTCTTGTTAGGG11.1363636363636365No Hit
GATCCCCACCGCACCCCCAGCCCGGCCGTGCGCCCCGCGCTCGCGGTCGA11.1363636363636365No Hit
CCCGTGTGCCCCACGTGCAAGGAGATCTACGAGGGCCTGCGCGAGCCGCA11.1363636363636365No Hit
GCCAAGGTACTTGCCCCCAATGATACCAGAATTGCGAACAGGAGGCTCAA11.1363636363636365No Hit
AACATGGAATGTTCTTCCATTTGTTTGTGTCCTCTTTTATCTCATTGAGC11.1363636363636365No Hit
ATCTTGGCTCACTGCAACCTCTGCCTCCCGGATTCAAGCAATTCTCCTGC11.1363636363636365No Hit
CGCTGGAGCTGCACCACCGCGAGACGGCCTTCGACGCATCGCGCCACCGG11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)