FastQCFastQC Report
Tue 10 May 2022
EGAF00003661475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57551
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG2060.35794338934162745No Hit
TGTCTGAGCTGATCCAAAAAAGCGTTGGGTTGGGGGAGAAGGAAATGATG1610.279751872252437No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1270.22067383711838195No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT1130.19634758735730048No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA1130.19634758735730048No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1120.19460999808865181No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1080.1876596410140571No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1060.18418446247675974No Hit
GTTTTATACTGAAATATGTCTGAGCTGATCCAAAAAAGCGTTGGGTTGGG1050.18244687320811107No Hit
GTGTAGCAGAAGAGGGTGAGAAGAAAATACTGGCGGGCAAGCTGGAAATG1010.17549651613351636No Hit
GTACATGGGAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCT930.16159580198432694No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA930.16159580198432694No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT910.1581206234470296No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT830.14421990929784018No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC820.1424823200291915No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA800.13900714149189417No Hit
GTACATGGGACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC720.12510642734270475No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA710.12336883807405605No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG710.12336883807405605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA700.12163124880540738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC700.12163124880540738No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT700.12163124880540738No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGC680.11815607026811002No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT680.11815607026811002No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT680.11815607026811002No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC670.11641848099946134No Hit
GTACATGGGAACCGCATGCCCAAGCCAGCCAAGACAGCCAAGAAGTGCTC660.11468089173081267No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC660.11468089173081267No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT640.11120571319351531No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG630.10946812392486664No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC620.10773053465621796No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG620.10773053465621796No Hit
GAAATATGTCTGAGCTGATCCAAAAAAGCGTTGGGTTGGGGGAGAAGGAA620.10773053465621796No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG610.10599294538756929No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC610.10599294538756929No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT610.10599294538756929Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGG610.10599294538756929No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG600.10425535611892062No Hit
GTACATGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC580.10078017758162326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)