FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003661849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003661849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGGGTAGAGGCCAGAGATCTGCTAAACGTCCTACGATGCACCGA42.898550724637681No Hit
ACTCTGTGAGTTGAATGCACACAACACAAGGAAGTTACTGGGAATTCCTC42.898550724637681No Hit
CTCAGTGAGATACAAGGGCCCAATAAAAAAAGAAAACTGCAGGTCAATGT32.1739130434782608No Hit
AGTGGCAAGAACTTCCCGGACGCACTAGCAGCGACAGGATTTTTTGTAAA32.1739130434782608No Hit
GTCTCGAGTGGAATGGAATAAACTCTAATGGAACAGACTGGAATGGACTC32.1739130434782608No Hit
GTGTATCAGATTTGACATCAAGTTTTCTCCCGTCAAAAGAATAAAAATGG32.1739130434782608No Hit
CCTGTCTCTTGGATATTCACTTGTGGAACTCAGTTTCCATGCTGTGAGAA32.1739130434782608No Hit
TTTGTAGACGAAAGAAATTTCAGAACTTGGCCAGAGGCTGCTGTCAGTAG21.4492753623188406No Hit
GTTTTATGAAAACCATCCAGCAGTACGAGGAGCCACCGTGTCAGAATCCC21.4492753623188406No Hit
TGCCGGTCATGCGCCGCTGCATCCTCAAGCCCTCCCAATCGCGCCGCCGG21.4492753623188406No Hit
CTTTTCTAAAGACATGAAATATTGAAACATTGCAAGTACTGATCCAGGTA21.4492753623188406No Hit
ACCCCGGCCTGAAGAACACGCTGGTGGTGGTCACGGCCGACCACGACCAT21.4492753623188406No Hit
ATATAGTACGGCACAAATATTAAGTGCTTTTTGTGATCATCTGTGTGTTA21.4492753623188406No Hit
CACCTTTCTTCTGCCTAACAGAGCCCTGAATTATTTAGTCACATGGTTTG21.4492753623188406No Hit
GCCTAGGCTGCTTCAATCACTGACGTCCTCGATGCCGGCTACCGATAGAG21.4492753623188406No Hit
CTAAATAGGTCAGTCACCTGGAAACCAATCCAGCACATGGGAAAAGAGAG21.4492753623188406No Hit
CAACGCGTGGTTTCCGGCCCAGGGGAAGGAACCATAAGGACCTCGCGCCA21.4492753623188406No Hit
TTTGTATGTTGATTAGGTATCCTAAAACTTTGCTTTTTATCAGCTAAAGG21.4492753623188406No Hit
GCGGAAGGTCATCGTCACCAGCACGGGGCCGAAGATCTCCTCGGTCGCGA21.4492753623188406No Hit
GGCCACAAGATCAGATGAGCCAGTTGATTGATCTGGTGGTGCCAGCTGAT21.4492753623188406No Hit
TCGCGGGTGACACCGGGTGTATCCTCGACGATGGCTTTTCTCTGCTCCAG21.4492753623188406No Hit
CGGGGTGTCCCTGTCGCTGGTGTTCGCTCTGCAGCTCGTCGACCTGTCTC21.4492753623188406No Hit
GTCTCACTTCCATATCGCCGAGCTCGCTTCGCCAGGGTTCTTGTACGACA21.4492753623188406No Hit
AGGATGGGGTGATGAGGAGGACAGTTCTGCTTACCATCAGTCTTGATGTC21.4492753623188406No Hit
GGACTCTTGTATACTAAATAGAAATTGTCATTAAATGGAGACTAAGCAAG21.4492753623188406No Hit
ATTCCACTCCATTCTATGCGATTTCATTCCAATCGATTCAATTCCATTCG21.4492753623188406No Hit
ATCCACCTGCGTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC10.7246376811594203No Hit
GGATGGATGACTCAACTGTTCCAGCCTGCAGGCTTTGGAGAGTCCAAGCT10.7246376811594203No Hit
GAATTTAAAGTCATGCCAATCTGACTAATTTCTTTCTGGCTCAAGTCTAG10.7246376811594203No Hit
GTATGTATTATCAGTTAATCACAAATTTTGTTCTTAGAGTTATATTTCTA10.7246376811594203No Hit
AAGGAGTCTCTCCTGAAGCTGTGAGCTGCATTGCCGAAGGTTGGGGGAGG10.7246376811594203No Hit
ACTCAGGACCAGGTTGTTGAGTTTCCATGTAGTTGATTGGTTTTGAGTGA10.7246376811594203No Hit
GAACACTTGAGGCCAGGAGTTCAAGACCAGCCTGGCCAACATGGGTTTAC10.7246376811594203No Hit
AGACCACTCATAGTTCAAAAGAATAGAGGGCCAATTAGATGGCTTTGAAA10.7246376811594203No Hit
GGCCTGAAGGGCGGCGAGCAGGTGGTGGTGAAGGGCGCTCTGCTGCTGGA10.7246376811594203No Hit
GTACTCTTCCGGTGTATCAGGGCGAAACAGAGGTGTGAAACCTTTATGCA10.7246376811594203No Hit
CTTTACAGATGCCATTCCATCATTACCTAGCTTTCATGAATTCTCTTAAA10.7246376811594203No Hit
GGATTAGCGTCGACAAAAAGTTCCGGTCTATTGTTTCAATTTCGTGAACA10.7246376811594203No Hit
CCAAAATCGTGCCACTGCACTCCAGCCTGGCGACAGAGTGAGACTGTCTC10.7246376811594203No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTAATTAAAAA10.7246376811594203No Hit
TGCCCGGACCCAGCCAACCAGCCCTATCGGGGAATTGCGCGGGGATAGCC10.7246376811594203No Hit
GCTAGACCTTGACCTTCAGTTTTCTGACTTATGCCCTGTTGGTCAGAAAA10.7246376811594203No Hit
GGACAGATGGAGAGAATAGCTCATCAGGGCCTTGGCTCTGAGGGCCCCTA10.7246376811594203No Hit
GTTGTTGTTGTTTTTTCTTTCTCCTAGGACCTTGTCTCTTTTTTTGAGCA10.7246376811594203No Hit
GATCTGCCCTGCCGCGCGGGAAGTCCAGTACGGCACGAGCCGCACGCGCT10.7246376811594203No Hit
TCATAGATTAGCCTAGCCTAGCTTAAATGTGCTGAGAACACTTACATTAG10.7246376811594203No Hit
AGTGCATTCCACTCCACTCCATTCAACTCCATTCCTTTCCGCTCCATTCC10.7246376811594203No Hit
CAGTGATTCCTCTGGACTCCATTTGATGATGATTCCATTCAATGATTCCA10.7246376811594203No Hit
GCGTGGTAGCACGTGCCTATAGTCCAAGCTATTTGGGAGGCTGAAGTGGG10.7246376811594203No Hit
AGACAGGGATGCCCTCTCTCACCACTCCTATTCCACATAGTGTTGGAAGT10.7246376811594203No Hit
GGGAGAGAGAGAGAGAGAGAGAGAAGCAGAAAGGGAGGGAGAGACAGAGA10.7246376811594203No Hit
CCAGAGCAGAAAAGCTGAAAATTCTAAAAATCAGAGTGACTCTTCTCCTC10.7246376811594203No Hit
TGCCAGTAAAGTGCAAGTTTCTTTTTCATTACAGTACCTTAAACAAATAA10.7246376811594203No Hit
TGATAGCGCAGCTTTGACACACTTTTTCTACAATGTGCAAGTGGCTATTT10.7246376811594203No Hit
GCAGAATACCTTCTAGGCATTGCCCTTTGAAGTAAACTTTTTGTTCCAGG10.7246376811594203No Hit
GATTCATTGAATGGAATCGGATGGAATCATCGAATGGACTGGAATGGAAT10.7246376811594203No Hit
GAGACTTTGCTGAAGTTGCTTATCAGCTTAAGGAGATGTTGGGCTGAGAC10.7246376811594203No Hit
CTATGAAACCAGTGTTTGAAAGATGATAACTTATCCTTTTGTGTTTTCTT10.7246376811594203No Hit
CAGCTGGGGCCGCAGCAGCGCATGCAGGTCATCACCGGCCCCAACATGGG10.7246376811594203No Hit
CCACGCCCGGCTAATTTTTGTATTTTTAGTAGATATGGGGTTTCGCCATG10.7246376811594203No Hit
GTGGTCTTCAGTGTGTTCCGATGCACACTTTTGCAGTCATGTGGGCATGC10.7246376811594203No Hit
GAATGCAGTCATCAAATGCTCTCGAATGGAATCCTCATCAAATGGAATCG10.7246376811594203No Hit
GGAGTGTAGTGGCATGATCTCGGCTCAAAAAAAAACAAAAAAACAAAAAA10.7246376811594203No Hit
ATATATGCCTATTTTTACACCATTACCATACTGTTTCAGTTACTGTGGCC10.7246376811594203No Hit
GACATGAGCACTAAAGTTTTGTTTTTAGGGAAGATCAATCTAGTGAGCAG10.7246376811594203No Hit
CACTTAAAGTGGGTAAAAGGAGGAGAGTTGGAAAGAAGATCTCTCCACAA10.7246376811594203No Hit
CCTTGTCGATGCGCACCGGTTCCAGGGCCCACGCCTGGCCCGGGTCAAGC10.7246376811594203No Hit
CCTTCAGAGTTTTATCTTTGTGCTTCAGTGCCCCACCATCACTGACAAGC10.7246376811594203No Hit
CATTTGATGATGATTCCATTCGATTCCATTTGATGATGATTCCATGCGAT10.7246376811594203No Hit
GGCAATCATGGTCGAATGGAATCAAATGGAAACAGCATTGAATAGAATTG10.7246376811594203No Hit
ATATATCATTGTTTAGGAAGTAGAGCATAAATAATTGTCATTAAATTCAG10.7246376811594203No Hit
GAAAAATGAACTATGTGTGTTACACTGAACCTAATTGTACCTGTCCAAAA10.7246376811594203No Hit
AGACGGGGTAGCGGCCCGCTTCAAGCGGGCGGAAGACGTCGGCACGCAAG10.7246376811594203No Hit
AGAATAATTTCTCACACTGTACAAATGCCAAAGATAGAAGTGAGTTTTTC10.7246376811594203No Hit
GTGCGGCAGATGCTGGCCTGGCTGGGCATGGCGTACCAGACGGTGCCGGT10.7246376811594203No Hit
GTGCGTGGTTGTCCCCTGGAAGTAGGTGGCCGCGAACTTGGCAAATTTAT10.7246376811594203No Hit
GACTTTTTGCTTTTATCTGTTCTGGTGGGTGTGACCTGCTATATTAGCAT10.7246376811594203No Hit
AGCTGGAGTGCAGTGGCGCAACTGGCTCACTGTAACCTCTGCCTCTCAGG10.7246376811594203No Hit
ATCATTGAAAGGAATTGAATGCAATCATTGAATGGAATAGAATGGAATCA10.7246376811594203No Hit
ATGTCCGGCACCGGTCTGCGCTGGAAGCCGCTGCGCGATCAGATCTTTTT10.7246376811594203No Hit
GCCTGGTATCTCTCAGCCTGAAACTTCTTTGGTATAATTTCTCTAGGAAA10.7246376811594203No Hit
CCGCAACCGGCGGGCGCCCTTCAAGCGCCATTCGCGCATCGTCGGCGGGC10.7246376811594203No Hit
ATCCTAGACTTTCCAGTTATGAGAAGGGAAGCATCATGCTGTAATTCCTG10.7246376811594203No Hit
ATGGAATACCATTAAGCAAACACATATTTATATTATGGAAATTTCAGAAG10.7246376811594203No Hit
AACCTGCTCTATGAACGGGAATGTTCAGCTCTGTGAGTTGAATGCAAACA10.7246376811594203No Hit
GTTTAATACTCAACTCAATCCTGAAGATATGCATCATGTTCCCATTTTAC10.7246376811594203No Hit
CTTCTATCCCGCGGGAGAGGAGAGTTTGAGTTCCGGGACCTGCGATTTTG10.7246376811594203No Hit
GAATTGAATGGAATCACCAATGAAGGGAATCAAATGGAATCCTCATTGAA10.7246376811594203No Hit
GGCATAAGGCACCGCACCCCGCCCTTTTTGTGCACTTTTTAAAGTTATAG10.7246376811594203No Hit
TGTAAGAGCTGTACGTGTTCATGAGGAAGATACTGCATTCAAAGAACGAA10.7246376811594203No Hit
AGATTCATCAGAGGAATCACTCTCTAAGTCAGCTATAGCCATATGAAATG10.7246376811594203No Hit
CTCAGAAACTTCTTTGTGATATCTGCATTCAAGTCACAGAGTTGAATATT10.7246376811594203No Hit
GTGTAAAAGTGTTCCTATTTCTCCACATCCTCTCCAGCACCTGTTGTTTC10.7246376811594203No Hit
CTGATGGGTTGCAGGAAGGGACCAAACAGAGGTATTTTCAATTTTTCATG10.7246376811594203No Hit
ATGATGTTCAGTGTTGACGACGAGCGCTCTCAGAGCGCAATCACATCCGC10.7246376811594203No Hit
CCCCAAGGGGAGAGGGAACAAAGCCCCCGGGCGGCCTCAGTCCGGCACGA10.7246376811594203No Hit
GGCTTATCTGGAACTCCTGACCTCAGGTGATCCACCCGCCTCGGCCTCCC10.7246376811594203No Hit
GAATGATTGGTGATGGCCTGGATACAGTTTTGTATGAATTGAAAAACTAA10.7246376811594203No Hit
CCAGAAACATTGGAGAAAGCTAAGGCTACCACCACCTACCCGGTCAGTCA10.7246376811594203No Hit
GCTCTGCTGCTTGCGCTTGGAACCTTGGGCTTTGCCGGCTACAGGATGGC10.7246376811594203No Hit
CAATTAAAACACATTCCCAGTGGGGGAGTCACATATTTTAGGATTCAAGC10.7246376811594203No Hit
TCCATGCTCTTCCCTGCACTTCCCAAAGATGGTGACTGGGTATAGAGAAG10.7246376811594203No Hit
GTCTTTAAGAGGTCTGGATGGGGAAATGGAGGGTGGGGCCAGGGAGACAG10.7246376811594203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)