FastQCFastQC Report
Tue 10 May 2022
EGAF00003662210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA700.8648381517173214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.7165801828514949No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA370.4571287373362985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.37064492216456635No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA300.37064492216456635No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA290.35829009142574747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.2841611069928342No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA210.25945144551519644No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA210.25945144551519644No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2470966147763776No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA200.2470966147763776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG200.2470966147763776No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA190.2347417840375587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG190.2347417840375587No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA180.2223869532987398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.21003212255992096No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC160.19767729182110205No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA160.19767729182110205No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC140.1729676303434643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT140.1729676303434643No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT140.1729676303434643No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA130.16061279960464542No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA130.16061279960464542No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT130.16061279960464542No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG120.14825796886582654No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA120.14825796886582654No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA120.14825796886582654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT120.14825796886582654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.14825796886582654No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT110.13590313812700766No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA110.13590313812700766No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT110.13590313812700766No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG100.1235483073881888Illumina PCR Primer Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT100.1235483073881888No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG100.1235483073881888No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT100.1235483073881888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT100.1235483073881888No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT100.1235483073881888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA100.1235483073881888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA90.1111934766493699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.1111934766493699No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA90.1111934766493699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.1111934766493699No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT90.1111934766493699No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG90.1111934766493699No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC90.1111934766493699No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCT90.1111934766493699Illumina Single End PCR Primer 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA90.1111934766493699No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT90.1111934766493699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)